º» Á¦Ç°Àº formalin-fixed, paraffin-embedded (FFPE) tissue·ÎºÎÅÍ qPCR, NGS, microarray µîÀÇ ½ÇÇè¿¡ ÀûÇÕÇÑ °íǰÁúÀÇ total RNA¸¦ ÃßÃâÇÏ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº À¯ÇØÇÑ phenol/chloroform ¶Ç´Â paraffin removal ¾øÀ̵µ ºü¸£°Ô °íǰÁúÀÇ total RNA¸¦ ÃßÃâÇÒ ¼ö ÀÖ´Ù.
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Features
miRCURY RNA Isolation Kit for FFPE´Â formalin-fixed, paraffin-embedded (FFPE) sample·ÎºÎÅÍ °íǰÁúÀÇ total RNA¸¦ ÃßÃâÇϱâ À§ÇÑ ÃÖÀûÀÇ Á¦Ç°ÀÌ´Ù. Unique spin column ±â¼ú ±â¹ÝÀÇ º» Á¦Ç°À» ÀÌ¿ëÇϸé FFPE »ùÇ÷κÎÅÍ 1½Ã°£ À̳»¿¡ ºü¸£°í ½ÉÇÃÇÏ°Ô ÃÖ°í È¿À²ÀÇ total RNA¸¦ ÃßÃâÇÒ ¼ö ÀÖ´Ù (±×¸² 1). De-paraffinization°ú lysis´Â ÀÎü¿¡ ¹«ÇØÇÑ blue-colored paraffin dissolver¸¦ »ç¿ëÇÏ¿© one stepÀ¸·Î ÁøÇàÇϱ⠶§¹®¿¡ RNA degradationÀ» ¹æÁöÇÒ »Ó¸¸ ¾Æ´Ï¶ó formalin cross-linkingÀ¸·ÎºÎÅÍ RNA¸¦ ºÐ¸®ÇÒ ½Ã¿¡ »ç¿ëµÇ´Â xylene, d-limonene ¶Ç´Â ´Ù¸¥ toxic reagent¸¦ »ç¿ëÇÒ Çʿ䰡 ¾ø´Ù. Spin columnÀº ¸Å¿ì ÀûÀº ¾çÀÇ »ùÇà (< 5 mg of tissue)µµ Àû¿ë°¡´ÉÇϸç, micro-dissection¿¡ ÃÖÀûÀÌ´Ù. º» Á¦Ç°Àº ¶ÇÇÑ vacuum systemÀ» Ȱ¿ëÇÑ automated processes ¿¡µµ Àû¿ë °¡´ÉÇÏ´Ù.


±×¸² 1. Workflow for RNA purification using the miRCURY RNA Isolation Kit - FFPE. Use the kit for rapid purification of total RNA from FFPE samples.

Product coverage
miRCURY RNA Isolation Kit for FFPE´Â formalin-fixed, paraffin-embedded (FFPE) »ùÇ÷κÎÅÍ °íÈ¿À²·Î RNA¸¦ ÃßÃâÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À» »ç¿ëÇÏ¿© ÃßÃâÇÑ RNA´Â RNA¿Í microRNA profiling¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.

Applications
Many labs and hospitals have access to vast numbers of formalin-fixed, paraffin-embedded (FFPE) archived samples. These samples contain well-preserved RNA and microRNA that are of clinical interest and which can be easily analyzed by gene expression profiling. The miRCURY¢â Isolation kit for FFPE makes exploration of the valuable developmental or clinical genetic information easily accessible as RNA fragments purified from the archived FFPE material are highly suitable for use in sensitive downstream gene expression analysis applications including qPCR, NGS and microarrays.

Exiqon offers RNA isolation from various sample types with superior yield and RNA quality for downstream RNA and microRNA profiling. Please see Figure 2 for advice on which kit to select for various sample sources.

±×¸² 2. Exosome and RNA isolation kits selection guide.
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Code

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300115

miRCURY RNA Isolation Kit - FFPE (50)

50 spin columns, reagents and buffers for total RNA isolation from FFPE samples. Including non-toxic Paraffin Dissolver.