특징
- Ecotropic lentivirus나 retrovirus을 이용하여 타겟 세포(Human cell을 포함)에 transduce시킬 때 사용
- 높은 형질도입 효율- 일시적으로 목적 cell의 viral receptor 의 농도를 증가 시켜는 원리
- 간단한 프로토콜- virus를 intectioin시키기 2시간 이전에 타겟 세포와 Ecotropic receptor booster(concentrated exosome-like vesicles)를 pre-incubation
Applications
그림. Receptor Boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus. Boosters result in increased
transduction efficiency because treatment increases the cell surface density of the receptor recognized by the lentivirus (ecotropic) or retrovirus
(ecotropic or amphotropic). Using this technology, for example, you can coat human cells with the ecotropic receptor (which is otherwise absent),
enabling them to be transduced with ecotropic retrovirus or ecotropic lentivirus.
그림. Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped
lentivirus. However, pre-treatment of these cell types with Ecotropic Receptor Booster allows for very efficient transduction.
그림. Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used. Panel A. HT1080 cells
were plated in 6-well plates 24 hr prior to transduction and incubated with 10 μl Ecotropic Receptor Booster for 2 hr. Cells were then transduced with
Lenti-X ZsGreen1 lentivirus (MOI=~15) produced using Clontech's Lenti-X HTX Ecotropic Packaging System and assayed 48 hr later for ZsGreen1 expression.
Panel B. A variety of human cell types that are normally resistant to transduction by ecotropic-pseudotyped lentiviral vectors were rendered susceptible
to transduction by pre-treatment with the Ecotropic Receptor Booster
Recommended for Producing Ecotropic Pseudotyped Virus:
- Ecotropic Lentivirus - Lenti-X™ Packaging single shots(Ecotropic)(Code 631278)
- Ecotropic Retrovirus - Retro-X Universal Packaging System (Code 631530)
