제품설명
Guide-it™ Cas9 Polyclonal Antibody
본 제품은 Streptococcus pyogenes 유래의 Cas9 단백질 검출을 할 수 있는 rabbit polyclonal antibody이며, 이는 Cas9 variants를 인식 가능하다 (Wild-type Cas9, Cas9 nickase, Cas9 nuclease-deficient mutants). 본 항체를 이용하면 Western blot 방법으로 대부분의 mammalian cell line에서 고감도, 낮은 background로 최소 0.5 ng의 재조합 Cas9을 검출 가능하다.

Guide-it™ Cas9 Monoclonal Antibody (Clone TG8C1)
본 제품은 Streptococcus pyogenes 유래의 full-length Cas9 재조합 단백질을 검출할 수 있는 mouse antibody이며 hybridoma cells로부터 생 성되었다. 본 항체는 western blot으로 mammalian cell lysate 또는 bacterial cell lysate로부터 최소 1ng의 야생형 (wilde-type)의 Cas9 단백질을 인식할 수 있다.



그림 1. The sensitivity of Cas9 polyclonal antibody assessed by Western blot. Various amounts of recombinant Cas9 (rCas9) were loaded (Lane 3: 0.15 ng, Lane 4: 0.30 ng, Lane 5: 0.625 ng, Lane 6: 1.25 ng, Lane 7: 2.5 ng, Lane 8: 5.0 ng, Lane 9: 10.0 ng, Lane 10: 20.0 ng). Cell lysates prepared from 3.5 x 104 cultured cells (Lane 1: HT1080 cells, Lane 2: HEK 293 cells) were used as a negative control. The blot was probed with Cas9 polyclonal antibody (1:1,000 dilution). For detection, an HRP-conjugated goat anti-rabbit secondary antibody was used (1:5,000 dilution), and signal was detected using ECL reagent.



그림 2. The specificity of Cas9 polyclonal antibody assessed by Western blot. Lysates prepared from various untransfected cell lines (3.5 x 104 cultured cells; Lane 1: HepG2, Lane 2: CHO-K1, Lane 3: HeLa, Lane 4: MCF7, Lane 5: HEK 293, Lane 6: HT1080, Lane 7: NIH 3T3, Lane 8: no cells) and HEK 293 cells expressing Cas9 from the CMV promoter (stable cell line; Lane 10: 2.0 x 103 cells, Lane 11: 5.0 x 103 cells, Lane 12: 1.0 x 104 cells, Lane 13: 1.5 x 104 cells). One nanogram of recombinant Cas9 (rCas) was used as a positive control (Lane 9). The blot was probed with Cas9 polyclonal antibody (1:1,000 dilution). For detection, an HRP-conjugated goat anti-rabbit secondary antibody was used (1:5,000 dilution), and signal was detected using ECL reagent. 



그림 3. Western blot analysis of the Guide-it™ Cas9 Monoclonal Antibody (Clone TG8C1). The Guide-it Cas9 Monoclonal Antibody (Clone TG8C1), which was raised in mouse against recombinant full-length Cas9 (rCas9), was tested via Western blot analysis using either increasing concentrations of recombinant Cas9 (Panel A) or lysates made from cells transiently expressing Cas9 after plasmid transfection (Panel B). Panel A: Increasing concentrations of rCas9 (1 ng, 2 ng, 3 ng, and 5 ng) were analyzed via Western blotting. Panel B: HEK 293 cells were transiently transfected with a plasmid encoding Cas9 protein.
적용
Western blot detection of Cas9 protein
Immunocytochemistry detection of Cas9 protein
보존
-20 ℃
구성품

Code

구성품

632607

100 μl Guide-it Cas9 Polyclonal Antibody (1 μg/μl)

632606

3 x 100 μl Guide-it Cas9 Polyclonal Antibody (1 μg/μl)

632628

100 μg Guide-it Cas9 Monoclonal Antibody (Clone TG8C1) (1 μg/μl)

632627

3 x 100 μg Guide-it Cas9 Monoclonal Antibody (Clone TG8C1) (1 μg/μl)