RiboGone-Mammalian kit는 human, mouse, rat의 total RNA 샘플로부터 5S, 5.8S, 18S, 28S nuclear rRNA (Ribosomal RNA) 뿐만
아니라 12S mtRNA (mitochondrial RNA)를 제거하는 시약이다. 특히, RiboGone은 FFPE 샘플과 같이 degradation된 샘플뿐만 아니라
낮은 quality의 RNA 샘플에도 적용 가능하다. RiboGone-Mammalian Kit는 random-primed cDNA synthesis와 RNA-seq 또는 transcriptom
분석 실험 전, sample preparation 과정에 적합하다 (including the SMARTer Stranded RNA-Seq Kit
(634836),
the SMARTer Universal Low Input RNA Kit for Sequencing
(634938), and the SMARTer Universal Low Input RNA Library Prep Kit (634947)) (그림 1).
그림 1. RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10-100 ng
of full-length or degraded total RNA. Samples processed using the RiboGone kit are ready for cDNA synthesis with any
random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing,
and the SMARTer Universal Low Input RNA Library Prep Kit).
특징
- Effective rRNA removal - rRNA removal method for human, mouse and rat total RNA samples - depletes ≥95% of ribosomal RNA sequences
- Suitable for any quality RNA - designed to work with any quality starting RNA, including degraded material like FFPE samples
- Low input RNA working range - 10 ng -100 ng starting material
- Works seamlessly with downstream random-primed cDNA synthesis kit like the Stranded RNA-Seq Kit or SMARTer Universal Low Input RNA Library Prep Kit
그림 2. Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment metrics
for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.
그림 3. Sequencing data of RiboGone - Mammalian treated RNA samples highly correlate with MAQC qPCR
data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows
that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.
적용
Removal of rRNA from small total RNA samples prior to random-primed cDNA synthesis
구성품 (자세한 구성품은 CoA 또는 매뉴얼 참조)
5X RiboGone Hyb Buffer
RNaseH (5 U/ul)
DNaseI (5 U/ul)
RNase Inhibitor (40 U/ul)
Nuclease-Free Water
RiboGone Purification Buffer
