Low Input Library Prep Kit v2 (Code 634899)는 Low Input Library Prep Kit (Code 634947)의 업그레이드 버전 제품입니다.

Double-stranded DNA를 Illumina platform 분석용 sequencing-ready library로 제작
Low input sample 적용: 약 50 pg의 dsDNA
Single-tube, three-step protocol eliminates intermediate purification steps and takes just 2 hours

Low Input Library Prep Kits는 미량의 DNA로부터 매우 민감하고 빠르며 단순하게 Illumina NGS 기기분석용 Multiplex library를 제작할 수 있는 제품이다. 본 제품은 Clontech의 NGS 분석제품인 SMARTer Universal Low Input RNA Kit for Sequencing (Code 634938), SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Code 634888) 외, 다양한 SMARTer 시리즈에 최적인 제품이다.

The Low Input Library Prep Kit (Cat. # 634947) produces libraries from dsDNA with up to 12 Illumina-compatible indexes. (This kit will be unavailable after 2015.)
The Low Input Library Prep Kit v2 (Cat. # 634899) produces Illumina-compatible libraries from a low amount of dsDNA. The kit comes with 12 indexing reagents pre-dispensed into tubes containing 8-nucleotide Sanger indexes that share the same sequences in the first 6 bases as the Illumina TruSeq LT indexes AS001 through AD012.
The Low Input Library Prep Kit HT (Cat. # 634900) has higher multiplexing capability and an optimized protocol for converting up to 96 low-input samples of fragmented, double-stranded DNA (dsDNA) into a sequencing-ready library for Illumina platforms. It comes with 96 dual-read, Illumina-compatible indexes pre-dispensed and sealed in microplates. The indexing reagent is a unique combination of Illumina's 8-nucleotide TruSeq HT i5 and i7 index sequences

그림 1. The Low Input Library Prep Kit workflow takes approximately 2 hours.