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TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0Àº plasma, whole blood, cell-free body fluids, virus stock solution, infected tissue¸¦ Æ÷ÇÔÇÑ ´Ù¾çÇÑ sample source·ÎºÎÅÍ virus RNA/DNA ¸¦ Á¤Á¦ÇÏ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº biological sample·ÎºÎÅÍ viral RNA/DNA¸¦ È¿À²ÀûÀ¸·Î Á¤Á¦ÇÒ ¼ö ÀÖ´Â nucleic acid preparation membrane°ú Ưº°ÇÑ lysis buffer·Î ±¸¼ºµÇ¾îÀÖ´Ù.
º» Á¦Ç°À» ÀÌ¿ëÇϸé virus lysis ÀÌÈÄ, 20ºÐ¸¸¿¡ °í¼øµµÀÇ RNA/DNA¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. Flury strain rabies virus·ÎºÎÅÍ 1.6 x 105 copies, Hepatitis A virus·ÎºÎÅÍ 1.0 x 104 copiesÀÇ RNA¸¦ ÃßÃâ °¡´ÉÇÏ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇÏ¿© ÃßÃâÇÑ RNA´Â RT-PCR, Northern blotting°ú °°Àº ¿©·¯ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÏ´Ù.
Flowchart
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¢º Part I
Proteinase K (20 mg/ml)

1 ml

Carrier RNA

50 ul



¢º Part II
Buffer VGB

12 ml

Buffer RWA*1

28 ml

Buffer RWB*2

24 ml

RNase free dH2O

2 ml x 2

Spin Columns

50

Collection tubes

50

RNase free collection tube (1.5 ml)

50


£ª1: Contains Strong denaturant. Be careful to avoid contacting with skin and eyes. In the case of such contact, wash immediately with plenty
      water and seek medical advice.
£ª2: Before using the kit, add 56 ml of 100% ethanol. Mix well.

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1. 100% ethanol
2. PBS solution
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1. Part I can be stored at -20¡É, and part II of the kit can be stored at room temperature (15-25¡É).
2. Part I can be shipped at -20¡É, and part II of the kit can be shipped at room temperature (15-25¡É).