ÃÖÀûÀÇ Lentivirus »ý»êÀ» À§ÇÑ workflow



Lentivirus production workflow

  1. Choose a virus packaging system type

  2. Select the right product

  3. Clone target gene

  • Any vector, Any insert, Anyone
  • 15ºÐ ³» cloning ÀÀ ¿Ï·á
  • 90% ÀÌ»óÀÇ ½ÇÇè Á¤È®µµ
    : ºÒÇÊ¿äÇÑ ¿°±â Ãß°¡°¡ ¾ø´Â Seamless cloning
  • Subcloning ¾øÀÌ ´Ù¾çÇÑ application Àû¿ë
  • Çâ»óµÈ colony Çü¼º ´É·ÂÀ¸·Î, ¼º°ø È¿À² Áõ°¡

  4. Package Lentivirus

  5. Titrate Lentivirus

Lentivirus´Â »ý¼º ÈÄ »óÃþ¾×À¸·Î ºÐºñµÇ±â ¶§¹®¿¡, ¼¼Æ÷ ÆÄ¼â¾øÀÌ °£ÆíÇÏ°Ô ¼¼Æ÷ »óÃþ¾×¿¡¼­ ¿ª°¡¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù.
ÀçÇö¼º ÀÖ´Â ½ÇÇè °á°ú¸¦ ¾ò±â À§Çؼ­´Â Á¤È®ÇÑ ¿ª°¡¸¦ ÃøÁ¤ÇÏ´Â °ÍÀÌ ÇʼöÀûÀÌ´Ù.

  6. Concentrate Lentivirus

  7. Transduce target cells with enhencer

  8. Analyze integration