·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
Products
°øÁö»çÇ×
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
樨毢
ȸ»ç¼Ò°³
That's Good Science!
Á¦Ç°¼±Åð¡À̵å
ÀüÁ¦Ç°º¸±â
Custom Service
À̺¥Æ®/Ä·ÆäÀÎ
´ÙÄ«¶ó¼Ò½Ä
´ÙÄ«¶ó°ø½Ã
½ÅÁ¦Ç°Á¤º¸
´ÙÄ«¶ó ¶óÀ̼±½º
±â¼úÁö¿ø¹®ÀÇ
Á¤º¸ÀÚ·á½Åû
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
Catalog
LS&B
ºê·Î¼Å
eNews
CoA & MSDS
¿Â¶óÀÎÅø
樨毢
¿Â¶óÀμ¼¹Ì³ª
ȸ»ç¼Ò°³
´ë¸®Á¡¾È³»
ã¾Æ¿À½Ã´Â±æ
Welcome
About Thats Good Science
Watch the Videos - S3
Watch the Videos - S2
Watch the Videos - S1
Home
>
ÀüÁ¦Ç°º¸±â
>
´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦
>
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
> His60 Ni Magnetic Beads
His60 Ni Magnetic Beads
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
635693
His60 Ni Magnetic Beads
1 ml
159,200¿ø
199,000¿ø
11.01 ~ 12.13
Clontech
635692
His60 Ni Magnetic Beads
10 mL
711,200¿ø
889,000¿ø
11.01 ~ 12.13
Clontech
635665
His60 Ni Buffer Set
20 ȸ
378,400¿ø
473,000¿ø
11.01 ~ 12.13
Clontech
631964
Magnetic Stand
1 Set
73,600¿ø
92,000¿ø
11.01 ~ 12.13
His-tagged proteinÀÇ ºü¸£°í °£ÆíÇÏ°Ô ºÐ¸®
His60 Ni°ú magnetic bead¸¦ ÀÌ¿ëÇÏ¿© °í¼øµµÀÇ ´Ü¹éÁú Á¤Á¦
ÀûÀº ºÎÇÇ¿¡¼elution
His60 Ni Magnetic Beads ´Â ClontechÀÇ His60 Ni Superflow Resin°ú magnetic beadÀÇ ºÐ¸®ÀåÁ¡À» °áÇÕÇÑ Á¦Ç°ÀÌ´Ù. BeadÀÇ ÀÚ¼º ÀÔÀÚ´Â magnetic separator¸¦ ÀÌ¿ëÇØ ¹Ì¼Ò±Ô¸ðÀÇ ´Ü¹éÁúÀ» ºü¸£°í ½±°Ô ºÐ¸®Çϵµ·Ï ÇÑ´Ù.
Highly Specific Binding & Elution
His60 Ni Magnetic Beads´Â ³ôÀº ƯÀ̼ºÀ¸·Î ÀÛÀº ºÐÀÚ·®¿¡¼ Å« ºÐÀÚ·®±îÁö ´Ù¾çÇÑ Å©±âÀÇ his-tagged protein°ú °áÇÕÇÑ´Ù. Á¤Á¦µÈ ´Ü¹éÁúÀº ¼Ò·®ÀÇ ¿ë¾× (50-200 ¥ìl)À¸·Î ¿ëÃâ°¡´ÉÇÏ¿© ³óÃàµÈ ÇüÅ·Π°á°ú°¡ ³ªÅ¸³´Ù (~3 mg/ml). His60 Ni Magnetic Beads´Â 5% Çöʾ×À¸·Î Á¦°øµÇ¸ç, ml´ç 1.65 mg ÀÇ ´Ü¹éÁú°ú °áÇÕÇÒ ¼ö ÀÖ´Ù.
Microscale Screening
His60 Ni Magnetic Beads´Â microscale Á¤Á¦´Â ¹ßÇö ¼öÁØÀ» ¼±º°Çϰųª protein-protein interaction ¿¬±¸¸¦ À§ÇØ »ç¿ëµÉ ¼ö ÀÖ´Ù. ¶ÇÇÑ His60 Ni ±â¼úÀÌ Á¢¸ñµÈ ClontechÀÇ Ç¥ÁØ His60 Ni Superflow ResinÀ» »ç¿ëÇÔÀ¸·Î½á ¸ñÀû ´Ü¹éÁú Á¤Á¦ÀÇ ±ÕÀÏÇÑ scale-upÀÌ °¡´ÉÇϵµ·Ï ÇÑ´Ù.
Applications
His60 Ni Magnetic Beads provide simple, effective separation of recombinant his-tagged proteins for a wide range of applications, including:
Microscale purification of his-tagged proteins
Studying protein structure and function
Preparing proteins for x-ray crystallography
Performing assays that detect protein-protein and protein-DNA interactions
Immunization to raise antibodies against a protein of interest
Screening for protein expression
Optimizing purification conditions for scale-up with His60 Ni Resin
His60 Ni Magnetic Beads can also be used to immobilize 6xHis-tagged proteins for affinity chromatography, in order to:
Analyze protein-protein and protein-nucleic acid interactions
Purify untagged subunits or nucleic acids that interact with the immobilized protein
Perform antibody purification
Study interactions between ligands and receptors
±×¸²1. Purifying his-tagged AcGFP1 from a cell lysate using His60 Ni Magnetic Beads under native conditions. 400 ¥ìl of clarified lysate from
E. coli
cells expressing AcGFP1-6xHis was added to His60 Ni Magnetic Beads and allowed to bind for 30 minutes. The beads were washed 3X with 500 ¥ìl of Wash Buffer, and eluted 4X with 100 ¥ìl of Elution Buffer (from the His60 Ni Buffer Set, Cat. No. 635655). A 10 ¥ìl aliquot of each sample was analyzed using a 12% SDS-PAGE gel. Lane M: Spectra Multicolor Broad Range Protein Ladder. Lane 1. Original sample. Lane 2. Flowthrough. Lane 3. Wash 1. Lane 4. Wash 2. Lane 5. Wash 3. Lane 6. Eluate 1. Lane 7. Eluate 2. Lane 8. Eluate 3. Lane 9. Eluate 4.
±×¸² 2. Purifying his-tagged AcGFP1 from a cell lysate using His60 Ni Magnetic Beads under denaturing conditions. 500 ¥ìl of clarified lysate from
E. coli
cells expressing AcGFP1-6xHis was added to His60 Ni Magnetic Beads and allowed to bind for 30 minutes. The beads were washed 3X with 500 ¥ìl of Equilibration Buffer and 1X with 500 ¥ìl of Wash Buffer-and eluted 3X with 50 ¥ìl pf Elution Buffer (from the His60 Ni Buffer Set, Cat. No. 635655). 8M urea was added to all the buffers. A 10 ¥ìl aliquot of each sample was analyzed using a 4-20% SDS-PAGE gel. Lane M: Spectra Multicolor Broad Range Protein Ladder. Lane 1. Original sample. Lane 2. Flowthrough. Lane 3. Wash 1. Lane 4. Wash 2. Lane 5. Wash 3. Lane 6. Wash 4. Lane 7. Eluate 1. Lane 8. Eluate 2. Lane 9. Eluate 3.
Product index
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
Brevibacillus ±Õü ³» ..
Brevibacillus ºÐºñ ¹ß..
Brevibacillus In vivo ..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
[°³¿ä] His-tag purific..
[FAQ] TALON¢ç Resin
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
Capturem¢â His-Tagged ..
TALON Resin
TALON Resin£¨Magnetic ..
HisTALON Cartridges & ..
xTractor Buffer Kit
His60 Ni Superflow Res..
His60 Ni Superflow Car..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
Chaperonin GroE
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
Protein Disulfide-isom..
Refolding CA Kit
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
´Ü¹éÁú ÃßÃâ ¹× ¶óº§¸µ ..
Calpastatin
Cryonase¢â Cold-active..
Exosome Isolation Kit ..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
Protein A Excellose (a..
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
Capturem¢â Protein A P..
Capturem¢â Protein G P..
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
Excellose Spin Kit His..
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
FLAG tag ´Ü¹éÁú Á¤Á¦
Anti-DYKDDDDK Antibody
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphopeptide ³óÃà Ki..
Phosphoprotein enrichm..
´Ü¹éÁú ¿°»ö ½Ã¾à
TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
´Ü¹éÁú °ËÃâ, Á¤·®, ÇØ¼®
HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
ProSieve¢â ProSieve EX..
Sodium Dodecyl Sulfate..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Asparaginylendopeptida..
Capturem¢â Trypsin and..
Capturem¢â trypsin dig..
Endoproteinase Asp-N
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
Pfu Pyroglutamate Amin..
Keyword :
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag Á¤Á¦Á¦Ç°
[°³¿ä] His-tag purification
[FAQ] TALON¢ç Resin
Â÷¼¼´ë His-tag purification system
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú Á¤Á¦, Capturem¢â
Spin columnŸÀÔÀ¸·Î ºü¸£°í °£ÆíÇÑ His-tag ´Ü¹éÁú..
Capturem¢â His-Tagged Purification
Histidine tag À¶ÇÕ ´Ü¹éÁúÀÇ °í¼øµµ Á¤Á¦¿¡
TALON Resin
TALON Resin£¨Magnetic Bead£©
TALON Resin ÃæÁøÇÑ Pre-packed type
HisTALON Cartridges & Gravity Columns
´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xTract..
xTractor Buffer Kit
Histidine tag À¶ÇÕ ´Ü¹éÁúÀÇ °í¼öÀ²·Î Á¤Á¦
His60 Ni Superflow Resin & Gravity Columns
His60 Ni resin ÃæÁøÁ¦ Pre-packed typ
His60 Ni Superflow Cartridges & Gravity Columns
His60 Ni Magnetic Beads
4¡É¿¡¼ Ȱ¼ºÀ» ¶ç´Â HN-tag¸¦ °®°í ÀÖ´Â ´Ü¹éÁú ºÐ..
HRV 3C Protease
Poly Histidine tag Ç×ü