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His60 Ni Magnetic Beads

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Clontech
635693
His60 Ni Magnetic Beads
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
1 ml
159,200¿ø 
199,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.13
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
His60 Ni Magnetic Beads Protocol-At-A-Glance_031716.pdf
Clontech
635692
His60 Ni Magnetic Beads
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
10 mL
711,200¿ø 
889,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.13
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635665
His60 Ni Buffer Set
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
20 ȸ
378,400¿ø 
473,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.13
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
His60 Ni Superflow Resin and Gravity Columns User Manual (PT5017-1)_031816.pdf
Clontech
631964
Magnetic Stand
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
1 Set
73,600¿ø 
92,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.13
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

  • His-tagged proteinÀÇ ºü¸£°í °£ÆíÇÏ°Ô ºÐ¸®
  • His60 Ni°ú magnetic bead¸¦ ÀÌ¿ëÇÏ¿© °í¼øµµÀÇ ´Ü¹éÁú Á¤Á¦
  • ÀûÀº ºÎÇÇ¿¡¼­elution
    His60 Ni Magnetic Beads ´Â ClontechÀÇ His60 Ni Superflow Resin°ú magnetic beadÀÇ ºÐ¸®ÀåÁ¡À» °áÇÕÇÑ Á¦Ç°ÀÌ´Ù. BeadÀÇ ÀÚ¼º ÀÔÀÚ´Â magnetic separator¸¦ ÀÌ¿ëÇØ ¹Ì¼Ò±Ô¸ðÀÇ ´Ü¹éÁúÀ» ºü¸£°í ½±°Ô ºÐ¸®Çϵµ·Ï ÇÑ´Ù.
Highly Specific Binding & Elution
His60 Ni Magnetic Beads´Â ³ôÀº ƯÀ̼ºÀ¸·Î ÀÛÀº ºÐÀÚ·®¿¡¼­ Å« ºÐÀÚ·®±îÁö ´Ù¾çÇÑ Å©±âÀÇ his-tagged protein°ú °áÇÕÇÑ´Ù. Á¤Á¦µÈ ´Ü¹éÁúÀº ¼Ò·®ÀÇ ¿ë¾× (50-200 ¥ìl)À¸·Î ¿ëÃâ°¡´ÉÇÏ¿© ³óÃàµÈ ÇüÅ·Π°á°ú°¡ ³ªÅ¸³­´Ù (~3 mg/ml). His60 Ni Magnetic Beads´Â 5% Çöʾ×À¸·Î Á¦°øµÇ¸ç, ml´ç 1.65 mg ÀÇ ´Ü¹éÁú°ú °áÇÕÇÒ ¼ö ÀÖ´Ù.

Microscale Screening
His60 Ni Magnetic Beads´Â microscale Á¤Á¦´Â ¹ßÇö ¼öÁØÀ» ¼±º°Çϰųª protein-protein interaction ¿¬±¸¸¦ À§ÇØ »ç¿ëµÉ ¼ö ÀÖ´Ù. ¶ÇÇÑ His60 Ni ±â¼úÀÌ Á¢¸ñµÈ ClontechÀÇ Ç¥ÁØ His60 Ni Superflow ResinÀ» »ç¿ëÇÔÀ¸·Î½á ¸ñÀû ´Ü¹éÁú Á¤Á¦ÀÇ ±ÕÀÏÇÑ scale-upÀÌ °¡´ÉÇϵµ·Ï ÇÑ´Ù.
Applications
His60 Ni Magnetic Beads provide simple, effective separation of recombinant his-tagged proteins for a wide range of applications, including:
  • Microscale purification of his-tagged proteins
  • Studying protein structure and function
  • Preparing proteins for x-ray crystallography
  • Performing assays that detect protein-protein and protein-DNA interactions
  • Immunization to raise antibodies against a protein of interest
  • Screening for protein expression
  • Optimizing purification conditions for scale-up with His60 Ni Resin

His60 Ni Magnetic Beads can also be used to immobilize 6xHis-tagged proteins for affinity chromatography, in order to:
  • Analyze protein-protein and protein-nucleic acid interactions
  • Purify untagged subunits or nucleic acids that interact with the immobilized protein
  • Perform antibody purification
  • Study interactions between ligands and receptors



±×¸²1. Purifying his-tagged AcGFP1 from a cell lysate using His60 Ni Magnetic Beads under native conditions. 400 ¥ìl of clarified lysate from E. coli cells expressing AcGFP1-6xHis was added to His60 Ni Magnetic Beads and allowed to bind for 30 minutes. The beads were washed 3X with 500 ¥ìl of Wash Buffer, and eluted 4X with 100 ¥ìl of Elution Buffer (from the His60 Ni Buffer Set, Cat. No. 635655). A 10 ¥ìl aliquot of each sample was analyzed using a 12% SDS-PAGE gel. Lane M: Spectra Multicolor Broad Range Protein Ladder. Lane 1. Original sample. Lane 2. Flowthrough. Lane 3. Wash 1. Lane 4. Wash 2. Lane 5. Wash 3. Lane 6. Eluate 1. Lane 7. Eluate 2. Lane 8. Eluate 3. Lane 9. Eluate 4.



±×¸² 2. Purifying his-tagged AcGFP1 from a cell lysate using His60 Ni Magnetic Beads under denaturing conditions. 500 ¥ìl of clarified lysate from E. coli cells expressing AcGFP1-6xHis was added to His60 Ni Magnetic Beads and allowed to bind for 30 minutes. The beads were washed 3X with 500 ¥ìl of Equilibration Buffer and 1X with 500 ¥ìl of Wash Buffer-and eluted 3X with 50 ¥ìl pf Elution Buffer (from the His60 Ni Buffer Set, Cat. No. 635655). 8M urea was added to all the buffers. A 10 ¥ìl aliquot of each sample was analyzed using a 4-20% SDS-PAGE gel. Lane M: Spectra Multicolor Broad Range Protein Ladder. Lane 1. Original sample. Lane 2. Flowthrough. Lane 3. Wash 1. Lane 4. Wash 2. Lane 5. Wash 3. Lane 6. Wash 4. Lane 7. Eluate 1. Lane 8. Eluate 2. Lane 9. Eluate 3.






Keyword :
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His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
> [¼±Åð¡À̵å] His-tag Á¤Á¦Á¦Ç°
> [°³¿ä] His-tag purification
> [FAQ] TALON¢ç Resin
Â÷¼¼´ë His-tag purification system
> [°³¿ä] Â÷¼¼´ë ´Ü¹éÁú Á¤Á¦, Capturem¢â
Spin columnŸÀÔÀ¸·Î ºü¸£°í °£ÆíÇÑ His-tag ´Ü¹éÁú..
> Capturem¢â His-Tagged Purification
Histidine tag À¶ÇÕ ´Ü¹éÁúÀÇ °í¼øµµ Á¤Á¦¿¡
> TALON Resin
> TALON Resin£¨Magnetic Bead£©
TALON Resin ÃæÁøÇÑ Pre-packed type
> HisTALON Cartridges & Gravity Columns
´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xTract..
> xTractor Buffer Kit
Histidine tag À¶ÇÕ ´Ü¹éÁúÀÇ °í¼öÀ²·Î Á¤Á¦
> His60 Ni Superflow Resin & Gravity Columns
His60 Ni resin ÃæÁøÁ¦ Pre-packed typ
> His60 Ni Superflow Cartridges & Gravity Columns
> His60 Ni Magnetic Beads
4¡É¿¡¼­ Ȱ¼ºÀ» ¶ç´Â HN-tag¸¦ °®°í ÀÖ´Â ´Ü¹éÁú ºÐ..
> HRV 3C Protease
> Poly Histidine tag Ç×ü
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