• His-tagged protein의 빠르고 간편하게 분리
• His60 Ni과 magnetic bead를 이용하여 고순도의 단백질 정제
• 적은 부피에서elution
  His60 Ni Magnetic Beads 는 Clontech의 His60 Ni Superflow Resin과 magnetic bead의 분리장점을 결합한 제품이다. Bead의 자성 입자는 magnetic separator를 이용해 미소규모의 단백질을 빠르고 쉽게 분리하도록 한다.

Highly Specific Binding & Elution

Microscale Screening

Applications

His60 Ni Magnetic Beads provide simple, effective separation of recombinant his-tagged proteins for a wide range of applications, including:

• Microscale purification of his-tagged proteins
• Studying protein structure and function
• Preparing proteins for x-ray crystallography
• Performing assays that detect protein-protein and protein-DNA interactions
• Immunization to raise antibodies against a protein of interest
• Screening for protein expression
• Optimizing purification conditions for scale-up with His60 Ni Resin

His60 Ni Magnetic Beads can also be used to immobilize 6xHis-tagged proteins for affinity chromatography, in order to:

• Analyze protein-protein and protein-nucleic acid interactions
• Purify untagged subunits or nucleic acids that interact with the immobilized protein
• Perform antibody purification
• Study interactions between ligands and receptors

그림1. Purifying his-tagged AcGFP1 from a cell lysate using His60 Ni Magnetic Beads under native conditions. 400 μl of clarified lysate from E. coli cells expressing AcGFP1-6xHis was added to His60 Ni Magnetic Beads and allowed to bind for 30 minutes. The beads were washed 3X with 500 μl of Wash Buffer, and eluted 4X with 100 μl of Elution Buffer (from the His60 Ni Buffer Set, Cat. No. 635655). A 10 μl aliquot of each sample was analyzed using a 12% SDS-PAGE gel. Lane M: Spectra Multicolor Broad Range Protein Ladder. Lane 1. Original sample. Lane 2. Flowthrough. Lane 3. Wash 1. Lane 4. Wash 2. Lane 5. Wash 3. Lane 6. Eluate 1. Lane 7. Eluate 2. Lane 8. Eluate 3. Lane 9. Eluate 4.



그림 2. Purifying his-tagged AcGFP1 from a cell lysate using His60 Ni Magnetic Beads under denaturing conditions. 500 μl of clarified lysate from E. coli cells expressing AcGFP1-6xHis was added to His60 Ni Magnetic Beads and allowed to bind for 30 minutes. The beads were washed 3X with 500 μl of Equilibration Buffer and 1X with 500 μl of Wash Buffer-and eluted 3X with 50 μl pf Elution Buffer (from the His60 Ni Buffer Set, Cat. No. 635655). 8M urea was added to all the buffers. A 10 μl aliquot of each sample was analyzed using a 4-20% SDS-PAGE gel. Lane M: Spectra Multicolor Broad Range Protein Ladder. Lane 1. Original sample. Lane 2. Flowthrough. Lane 3. Wash 1. Lane 4. Wash 2. Lane 5. Wash 3. Lane 6. Wash 4. Lane 7. Eluate 1. Lane 8. Eluate 2. Lane 9. Eluate 3.