Lentiviral Packaging Systems

Lenti-X high-titer lentiviral packaging systems generate lentiviral titers that are superior to most other commercially available lentiviral packaging systems. At titers of 10⁷-10⁸ infectious units (IFU) per ml, you can often transduce an entire dish of target cells using only 10-100 ul of unconcentrated lentiviral packaging cell supernatant.

• No need to concentrate virus
• No need to transfect multiple plates
• No need to infect your target cells multiple times
• Easily control the expression level of your gene of interest by manipulating the multiplicity of infection (MOI)

Lentiviral Packaging Systems-Two Options

The Lenti-X HTX Packaging System is available in two pseudotyping options:

1. VSV-G lentiviral packaging-The standard and most popular Lenti-X HTX Packaging System produces VSV-G pseudotyped lentivirus,
    which readily infects virtually all types of cells.
2. Ecotropic lentiviral packaging-The Lenti-X HTX Ecotropic Packaging System produces lentivirus pseudotyped with the MLV
    ecotropic envelope glycoprotein, which allows you to limit transduction to mouse and rat cells.

How Are High Lentiviral Titers Achieved?

• Optimized composition-Our novel 4^th Generation Lentiviral Packaging Mixes provide multiple lentiviral packaging and nonviral components in a proprietary suite of 5 vectors that are premixed in specific, ideal ratios in order to maximize virus production. Multiple vectors also effectively prevent the emergence of replication competent viruses.
  
  
• Tetracycline transactivation-Very strong expression of several key lentiviral packaging components is produced by Tet-Off transactivation of tetracycline-responsive promoter elements (TREs). In many cell lines, including HEK 293 cells, the tetracycline transactivator generates absolute expression levels much higher than those produced by the CMV promoter.
  
  
• Optimized transfections-Our exceptional, nanoparticle-based transfection reagent, Xfect, is included with every lentiviral packaging system. This reagent consistently transfects Lenti-X 293T cells with very high efficiencies (> 95%) and allows high expression of viral components.
  
  
• Simple monitoring of lentivirus production-Lenti-X GoStix (included) let you instantly determine the optimal time to harvest your lentiviral supernatant.

The concerted effects of these advanced components allow Lenti-X 293T Cells (sold separately) to produce the highest amounts of safe, replication-incompetent lentivirus from any of our lentiviral vectors, or any other lentiviral vector.

The viruses are perfect for transducing delicate, hard-to-transfect cell types, such as primary neuroprogenitor cells. The standard Lenti-X HTX Packaging System is supplied with many of our lentiviral expression systems. Visit our lentiviral vectors page to see the variety of vectors and systems available.