2017³â ´ÙÄ«¶óÄÚ¸®¾Æ¿¡¼ »õ·Ó°Ô °ø±ÞÇÏ´Â ºê·£µå Rubicon, WaferGen, Ajinomoto¸¦ ¼Ò°³ÇÕ´Ï´Ù.
ºê·£µå¸í : Rubicon (Rubicon Genomics)
Á¦Ç°Á¤º¸
1) ÁÖ¿ä Á¦Ç° : DNA¸¦ ÀÌ¿ëÇÏ¿© NGS¿ë library¸¦ Á¦ÀÛÇϱâ À§ÇÑ Á¦Ç°
2) Ư¡ : ´Ù¾çÇÑ Á¾·ùÀÇ DNA(genomic DNA, cDNA, cell free DNA, FFPE¿¡¼ ÃßÃâÇÑ DNA µî)¸¦ ÀÌ¿ëÇÏ¿© °£ÆíÇÏ°Ô(single tube reaction) library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖÀ½.
3) Á¦Ç° ¶óÀÎ : Å©°Ô 3°³ÀÇ Á¦Ç° ¶óÀÎÀ¸·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç, Á¦Ç°¸¶´Ù ´Ù¾çÇÑ ¿ë·® ¹× index º¸À¯
ÁÖ¿äÁ¦Ç°
1) ThruPLEX¢ç DNA-Seq ½Ã¸®Áî : ´Ù¾çÇÑ DNA(gDNA, cDNA, ChIP-DNA, cell free DNA µî)¸¦ ÀÌ¿ëÇÏ¿© NGS ºÐ¼®À» À§ÇÑ library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â Á¦Ç°À¸·Î,
single tube reactionÀ̱⠶§¹®¿¡ ¿À¿° ¹× »ùÇà ¼Õ½Ç(loss)À» ÃÖ¼ÒÈ ÇÒ ¼ö ÀÖ´Ù.
2) PicoPLEX¢ç DNA-Seq : single cell ȤÀº ±Ø¼Ò·®ÀÇ DNA¿¡¼ NGS¿ë library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖÀ¸¸ç, ThruPLEX kit¿Í µ¿ÀÏÇÏ°Ô single tube reactionÀÌ´Ù.
3) PicoPLEX¢ç WGA Kit : whole genome amplificationÀ» À§ÇÏ¿© Àü¼¼°èÀûÀ¸·Î ¸¹ÀÌ »ç¿ëµÇ°í ÀÖ´Â Á¦Ç°À¸·Î, IVF½Ã pre-implantation genetic screening,
diagnosis(PGS, PGD)¿¡ ³Î¸® »ç¿ëµÇ°í ÀÖ´Ù.
ºê·£µå¸í : WaferGen (WaferGen Biosystems)
Á¦Ç°Á¤º¸
1) ÁÖ¿äÁ¦Ç° : Single cell analysis, High-throughput Real Time PCR ½Ã½ºÅÛ, automation NGS preparation system
2) Ư¡ : 5,184 °³ÀÇ nano-wellÀ» °¡Áö°í ÀÖ´Â SmartChip Àû¿ë
ÁÖ¿äÁ¦Ç°
1) iCELL8 Single cell system : SmartChipÀ» ÀÌ¿ëÇÏ¿© single cell ºÐÁÖ – È®ÀÎ – ¹ÝÀÀ±îÁö °¡´ÉÇÏ°Ô ÇÏ´Â complete system
2) SmartChip¢â MyDesign Real-Time System : SmartChipÀ» ÀÌ¿ëÇÏ¿© Ãʹ̷® »ùÇðú ¹ÝÀÀ¾×À¸·Î high-throughput Real time PCR ºÐ¼® °¡´É
3) Apollo324 : autiomation NGS Library preparation system
ºê·£µå ¹× Á¦Ç°¸í : Ajinomoto StemFit¢ç Basic02
StemFit¢ç Basic02´Â ±³Åä´ë iPS¼¼Æ÷¿¬±¸¼Ò CiRA¿Í °øµ¿¿¬±¸·Î °³¹ßµÈ hiPSC¿ë ¹èÁö·Î, Â÷¼¼´ë hiPSC ¿¬±¸¸¦ À§ÇØ ±âÁ¸ stem cell Àü¿ë ¹èÁö¿¡¼ ºÒ°¡´É ÇÏ¿´´ø single cell expansion ´É·ÂÀ» ±¸ÇöÇÏ¿´°í Xeno-free, Feeder-free mediaÀÌ´Ù.
º» Á¦Ç°°ú CRISPR/Cas9À» »ç¿ëÇÏ¿© ±ÕÀÏÇÏ°Ô À¯Àüü ÆíÁýµÈ hiPSCÀ» Á¦ÀÛ, ¹è¾çÇÒ ¼ö ÀÖÀ¸¸ç reprogramming, expansion, differentiationÀÇ hiPSC ¿¬±¸ÀÇ
¸ðµç ´Ü°è¿¡ »ç¿ë °¡´ÉÇÏ´Ù.