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Next generation sequencing has led to diverse RNA-Seq methods, but one drawback is sensitivity. Conventional RNA-Seq requires >10 ng of RNA from 50-100 cells. But increasingly researchers are interested in profiling gene expression in single cells. Ramskold and colleagues used the SMARTer® Ultra™ Low Input RNA Kit for Illumina® sequencing to investigate gene expression in a single circulating tumor cell, starting with a mere 10 pg of RNA. - That's Good Science!™

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