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Home > ÀüÁ¦Ç°º¸±â > ±â±â ¡¤ ¼Ò¸ðÇ° > NGS Library prep ÀåÄ¡ > Apollo¢â Library Prep System Àü¿ë ½Ã¾à

Apollo¢â Library Prep System Àü¿ë ½Ã¾à

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
640101
PrepX¢â DNA Library Kit (400075)
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 samples
°¡°Ý¹®ÀÇ  
Takara
640102
PrepX¢â DNA Library Kit (400076)
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 samples
(Package)
°¡°Ý¹®ÀÇ  
Takara
640103
PrepX¢â ILMN Barcodes 1-24 (400077-1)
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 barcodes
°¡°Ý¹®ÀÇ  
Takara
640107
PrepX¢â ILMN Barcodes 1-96 (400077-5)
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 barcodes
°¡°Ý¹®ÀÇ  

Á¦Ç°¼³¸í
PrepX ½Ã¸®Áî´Â Apollo¢â library Prep System Àü¿ë kit·Î¼­, Apollo¢â ½Ã½ºÅÛ¿¡ ÃÖÀûÈ­µÈ DNA/RNA-Seq¿ë Library Preparation kitÀÌ´Ù. ¸ðµç PrepX ½Ã¸®Áî´Â Apollo¢â ½Ã½ºÅÛ¿¡ ÃÖÀûÈ­µÈ protocolÀÌ ³»ÀåµÇ¾î ÀÖ¾î, º°µµÀÇ Script Á¦ÀÛ ¾øÀÌ »ç¿ëÀÌ °¡´ÉÇÏ´Ù.
Apollo¢â ½Ã½ºÅÛ GUI¿¡¼­ ÇØ´çÇÏ´Â Kit¿Í »ùÇà ¼ö¸¦ ¼±ÅÃÇϸé ÇØ´çÇÏ´Â °¢°¢ÀÇ component À§Ä¡¿Í ¼ö·®À» ÀÚµ¿À¸·Î ¼³Á¤, ¾Æ·¡ÀÇ ±×¸²1.°ú °°ÀÌ Apollo¢â ½Ã½ºÅÛ GUI»ó¿¡ Ç¥½ÃÇÑ´Ù. Kit component¿Í plastic ware¸¦ ±×¸²¿¡ µû¶ó ÀåÂøÇÑ ÈÄ RunÀ» ½ÇÇàÇÏ´Â °Í¸¸À¸·Î ½Ã½ºÅÛ Á¶ÀÛÀÌ ¿Ï·áµÃ´Ù.Gui¿¡ µû¶ó ÁøÇàÇϹǷΠ¼³Ä¡ ½Ã ¹ß»ýÇÒ ¼ö ÀÖ´Â »ç¿ëÀÚÀÇ ¿À·ù¸¦ Â÷´ÜÇÏ¿© Ç×»ó ÃÖ»óÀÇ °á°ú¸¦ È®º¸ÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ ¸ðµç PrepX¢â ½Ã¸®Áî´Â Library Á¦ÀÛ¿¡ ÇÊ¿äÇÑ reagent¿Ü¿¡ bead, adaptor, ligase, polymerase, primer, index µîÀÌ ¸ðµÎ Æ÷ÇԵǾî ÀÖ´Â, all-in-one kitÀÌ´Ù. ¸ðµç PrepX kit¢â´Â ±×¸²2.¿Í °°ÀÌ °¢ componenetº° ´Ù¸¥ »öÀÇ Strip ÇüÅ·ΠÁ¦°øµÇ°í ÀÖ´Ù.


<±×¸²1. PrepX¢â Library Prep SystemÀÇ GUI>                     <±×¸²2. PrepX reagent>
Á¦Ç°Æ¯Â¡
Code
Á¦Ç°¸í
ÇØ´ç protocol
»ùÇüö
»ùÇÃ Input (ÃÖ¼Ò)
ouptut size
Run Time on Apollo System
RNA-Seq
640096
PrepX RNA-Seq for Illumina Library Kit, 24 Samples
PrepX mRNA 8
1-8
100pg polyA-enriched RNA
2ng rRNA-depleted mRNA
~200-800 bp
5 hr
640097
PrepX RNA-Seq for Illumina Library Kit, 48 Samples
PrepX mRNA 48
6-48
100pg polyA-enriched RNA
2ng rRNA-depleted mRNA
~200-800 bp
6 hr 15 min
DNA-Seq
640101
PrepX Complete ILMN DNA Library Kit, 24 Samples
PrepX ILM 8
1-8
1ng DNA
220 bp
320 bp
520 bp
870 bp
1 hr 20 min
640102
PrepX Complete ILMN 32i DNA Library Kit, 96 Samples
PrepX ILM 32i
4-32
1ng DNA
220 bp
320 bp
520 bp
870 bp
3 hr 20 min

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