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Single cell seq °¡À̵å

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Single cell-seq

´ÜÀϼ¼Æ÷ (Single cell) ºÐ¼®Àº ¼¼Æ÷ÀÇ ±ºÁý (cell population)ÀÌ ¾Æ´Ñ ¼¼Æ÷ Çϳª Çϳª¸¦ ¿¬±¸ÇÏ´Â ¹æ¹ýÀ¸·Î½á, ¼¼Æ÷ °¢°¢ÀÇ °íÀ¯ÇÑ »ý¹°ÇÐÀû Ư¼ºÀ» ±Ô¸íÇÒ ¼ö ÀÖ´Ù. ÀÌ´Â Áٱ⼼Æ÷, ¼øȯÁ¾¾ç¼¼Æ÷ (Circulating Tumor Cells; CTC), Á¶Á÷»ý°Ë (tissue biopsy)°ú °°Àº ¾çÀÌ ¾ÆÁÖ Àû°í ±ÍÁßÇÑ »ùÇÃÀ» ºÐ¼®ÇÏ´Â µ¥ ¸Å¿ì Áß¿äÇÏ´Ù.
Single cellÀ» º¸´Ù ¹Î°¨ÇÏ°í ÀçÇö¼º ³ô°Ô ºÐ¼®Çϱâ À§ÇÑ ¡®SMART¡¯ÇÑ ¡®SMARTer Á¦Ç° ½Ã¸®Á¸¦ ¼Ò°³ÇÑ´Ù.

Single cell ºÐ¼® ºÐ¾ß

Single cellÀº À¯ÀüüÇÐ (Genomics), Àü»çüÇÐ (Transcriptomics), ´Ü¹éÁúüÇÐ (Proteomics)°ú °°ÀÌ ´Ù¾çÇÑ Çй®¿¡¼­ ºÐ¼® ¡¤ Àû¿ëµÉ ¼ö ÀÖ´Ù. ƯÈ÷, DNA ¼öÁØÀ» ¿¬±¸ÇÏ´Â À¯ÀüüÇаú RNA ¼öÁØÀ» ¿¬±¸ÇÏ´Â Àü»çüÇÐÀº ÃÖ½ÅÀÇ ¼­¿­ºÐ¼®±â¹ýÀÎ NGS (Next Generation Sequencing) ¹æ¹ýÀ» ÁÖ·Î ÀÌ¿ëÇÏ°í ÀÖÀ¸¸ç, ÀÌ´Â ¡®Single cell-seq¡¯À¸·Î Åë¿ëµÈ´Ù.



Single cell-seq ½ÇÇè °úÁ¤

Single cell À¯Àüü¸¦ ºÐ¼®Çϱâ À§ÇÑ ½ÇÇèÀº ÀϹÝÀûÀ¸·Î ¡®Single cell ºÐ¸® -> À¯Àü¹°ÁúÀÇ ÁõÆø -> NGS Library Preparation -> Sequencing¡¯ ¼ø¼­·Î ÁøÇàµÈ´Ù.
¼¼Æ÷ Çϳª¿¡¼­ NGS ºÐ¼®¿¡ ÃæºÐÇÑ ¾çÀ» ¾ò±â À§ÇÑ Amplification °úÁ¤Àº RNAÀÏ °æ¿ì Whole Transcriptome Amplification (WTA), DNAÀÏ °æ¿ì Whole Genome Amplification (WGA)À̶ó´Â Ư¼öÇÑ ÁõÆø ±â¼úÀ» »ç¿ëÇÑ´Ù.



Single cell-seq Portfolio

´ÙÄ«¶óÄÚ¸®¾Æ´Â Single cell ºÐ¸®ºÎÅÍ DNA ¡¤ RNA Amplification, NGS Library Preparation (for Illumina¢ç platform)±îÁö °¡´ÉÇÑ Á¦Ç°À» Á¦°øÇÏ°í ÀÖ´Ù. That¡¯s Good Science!
* Á¦Ç°¸íÀ» Ŭ¸¯ÇÏ½Ã¸é º¸´Ù »ó¼¼ÇÑ Á¦Ç°Á¤º¸¸¦ È®ÀÎÇÒ ¼ö ÀÖ½À´Ï´Ù.

¡¤ ´ÜÀϼ¼Æ÷ ºÐ¸® (Single cell sorting / isolation)

High-throughput single cell isolation, selection, and processing
ICELL8¢ç cx Single-Cell System (Code 640189)

  • Çѹø¿¡ ÃÖ´ë 1,200 ~ 1,500°³ live single cell ºÐ¸®
  • ¼¼Æ÷ Å©±â¿¡ »ó°ü¾øÀÌ ºÐ¸® °¡´É (5 - 100 §­)
  • Imaging stationÀ» ÀÌ¿ëÇÏ¿© ¡®»ì¾ÆÀÖ´Â single cell¡¯¸¸ ¼±ÅÃÇÏ¿© ºÐ¼®
  • Single cell (´ÜÀϼ¼Æ÷) ºÐ¸®¿¡¼­ ¡®»ì¾ÆÀÖ´Â single cell¡¯ ÆÇÁ¤±îÁö ´Ü 30ºÐ ¼Ò¿ä!
  • Àû¿ë½ÇÇè: Differential expression (DE), TCR profiling, RNA-seq, ATAC-seq



¡¤ DNA ºÐ¼® (Single cell WGA & DNA-Seq)

Gold standard WGA, PicoPLEX ±â¼úÀ» Á¢¸ñÇÑ Single Cell NGS Library Prep
PicoPLEX¢ç Gold Single Cell DNA-seq Kit

  • Whole Genome Amplification (WGA)À» ÀÌ¿ëÇÏ¿© Single cell DNA ºÐ¼® (6 pg of DNA ºÐ¼®°¡´É)
  • Single cell CNV, SNV, Aneuploidy °ËÃâ¿¡ Ź¿ù
  • ü¿Ü¼öÁ¤¶õÀÇ Âø»óÀüÀ¯ÀüÀÚ°Ë»ç (PGS/PGD)¿¡¼­ °¡Àå Àß ¾Ë·ÁÁø ºÐ¼® ±â¼ú



¡¤ RNA ºÐ¼® (Single cell WTA & RNA-Seq)

Single cell Àü¿ë SMART-Seq¢ç



Ultra low inputÀ» À§ÇÑ SMART-Seq¢ç v4 ½Ã¸®Áî

  • Ultra low input (10 pg - 10 ng) À¸·ÎºÎÅÍ Full-length cDNA ÇÕ¼º ¹× ÁõÆø; Whole Transcriptome ºÐ¼®
  • Single cell, Ultra-low input (10 pg) RNA ºÐ¼®ÀÇ ±âÁØ
  • ¼ö¸¹Àº ³í¹®°ú ¿¬±¸ÀÚ°¡ ÀÎÁ¤ÇÏ´Â °­·ÂÇÑ Single cell ºÐ¼® Åø



Single cell-seq ÃֽŠÁ¤º¸ ÀÚ·á