´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > Overview > Real Time PCR accessories

Real Time PCR accessories

-
Real Time PCR normalization & primer set
Real Time PCR normalization
´ÙÄ«¶ó¿¡¼­´Â ´Ù¾çÇÑ Á¶Á÷¿¡¼­ À¯·¡ÇÑ reference RNA¿Í cDNA¸¦ ÆǸÅÇÏ°í ÀÖ¾î ´Ù¾çÇÑ Real Time PCR ½ÇÇè¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.

Code

Á¦Ç°¸í

¿ë·®

Reference RNA

636690

qPCR Human Reference Total RNA

25 §¶

636657

Mouse Universal Reference Total RNA

200 §¶ ¡¿2

636658

Rat Universal Reference Total RNA

200 §¶ ¡¿2

Reference cDNA

636692

qPCR Human Reference cDNA, oligo(dT)-primed

25 ȸ

636693

qPCR Human Reference cDNA, oligo(dT)-primed

100 ȸ

639653

qPCR Human Reference cDNA, random-primed

25 ȸ

639654

qPCR Human Reference cDNA, random-primed

100 ȸ


Real Time PCR Áß ¸ñÀûÀ¯ÀüÀÚÀÇ ¹ßÇö·®À» reference geneÀÇ ¹ßÇö·®À¸·Î º¸Á¤ÇÏ¿© »ó´ëÀûÀÎ Á¤·®°ªÀ» »êÃâÇÏ´Â ¹æ¹ýÀÌ ³Î¸® »ç¿ëµÈ´Ù (»ó´ë Á¤·®¹ý). ÀÌ °æ¿ì house keeping geneÀ» reference geneÀ¸·Î ÁÖ·Î »ç¿ëÇÏÁö¸¸, house keeping geneÀÇ ¼±Á¤ÀÌ ¾î·Á¿î °æ¿ì, ´ë½Å external RNA¸¦ ½ÇÇè¿¡ ÷°¡ÇÏ¿© reference geneÀ¸·Î ´ëüÇÒ ¼ö ÀÖ´Ù.
´ÙÄ«¶ó¿¡¼­´Â Real Time RT-PCRÀ» À§ÇÑ external RNA¿Í dilution solution, °ËÃâ¿ë primer set·Î ±¸¼ºµÈ External Standard Kit (Lambda PolyA) for qPCR (Code 3789)¸¦ ÆǸÅÇÏ°í ÀÖ´Ù. ¥ë DNA¸¦ ÁÖÇüÀ¸·Î in vitro transcription¿¡¼­ ÇÕ¼ºÇÑ ¾à 1.0 kbÀÇ RNA (poly A°¡ Æ÷ÇÔ)¸¦ Æ÷ÇÔÇÏ´Â º» Á¦Ç°Àº eukaryotic gene ¹ßÇö ¿¬±¸¿¡ external reference·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù.

Code

Á¦Ç°¸í

¿ë·®

3789

External Standard Kit (¥ëpoly A) for qPCR

20 ȸ

9160

EASY Dilution (for Real Time PCR)

8 x 1 §¢


ÇÔ²² Æ÷ÇԵǾî ÀÖ´Â EASY Dilution (for Real Time PCR) (º°µµ ±¸¸Å½Ã Code 9160)Àº total RNA³ª cDNA¸¦ ´Ü°è Èñ¼® (serial dilution)ÇÒ ¶§ Èñ¼®¿ë¾×À¸·Î »ç¿ëÇÑ´Ù. H2O³ª TE buffer¸¦ »ç¿ëÇßÀ» ¶§º¸´Ù Àú³óµµ±îÁö Á¤È®ÇÏ°Ô Èñ¼®ÇÒ ¼ö ÀÖ°í, ¿ªÀü»ç ¹ÝÀÀÀ̳ª PCRÀÇ ¹ÝÀÀ¼º¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾ÊÀ¸¹Ç·Î Èñ¼®ÇÑ ÁÖÇü ¿ë¾×À» ±×´ë·Î ¿ªÀü»ç ¹ÝÀÀÀ̳ª PCR ¹ÝÀÀ¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù.

Real Time PCR primer sets
PrimerArray Disease½Ã¸®Áî´Â ¾Ï, ´ç´¢º´, õ½Ä µî Áúȯ¿¡ °ü·ÃµÈ human ¶Ç´Â mouse À¯ÀüÀÚ ¹ßÇö Çؼ®À» À§ÇÑ Real Time RT-PCR¿ë primer setÀÌ´Ù. °¢°¢ 88Á¾ÀÇ °ü·Ã À¯ÀüÀÚ¿Í 8Á¾ÀÇ house keeping geneÀÇ °ËÃâÀ» À§ÇÑ primer°¡ Æ÷ÇԵǾî ÀÖ¾î, »ó´ëÁ¤·®¹ýÀ» ÀÌ¿ëÇÑ »ùÇà °£ À¯ÀüÀÚ ¹ßÇö º¯È­¸¦ ½ºÅ©¸®´×ÇÒ ¼ö ÀÖ´Ù.
°¢ Primer set´Â Real Time RT-PCR¿¡ ¾çÈ£ÇÏ°Ô ÁõÆøµÊÀ» È®ÀÎÇÏ¿´°í, PrimerArray Analysis Tool Ver.2.2*¶Ç´Â Multiplate RQ*·Î Çؼ®ÇÒ ¼ö ÀÖÀ¸¸ç, °¢°¢ÀÇ primer¸¦ º°µµ ±¸ÀÔÇÏ¿© ¹ßÇöº¯È­°¡ È®ÀÎµÈ À¯ÀüÀÚ¿¡ ´ëÇÑ Ãß°¡ ½ÇÇèÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
*Çؼ®À» À§ÇÑ ÇÁ·Î±×·¥Àº ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ·Î ¹®ÀÇ ¹Ù¶ø´Ï´Ù. T: 02-2081-2510, support@takara.co.kr

PrimerArray Á¦Ç°¶óÀÎ ¹× Primer ¸®½ºÆ®

Ä«Å×°í¸®

Human Á¦Ç°ÄÚµå*

Mouse Á¦Ç°ÄÚµå*

Cytokine-cytokine receptor interaction

PH001

PN001

Cell cycle

PH002

PN002

Cell adhesion molecules

PH003

PN003

Jak-STAT signaling pathway

PH004

PN004

Natural killer cell mediated cytotoxicity

PH005

PN005

Axon guidance

PH006

PN006

Focal adhesion

PH007

PN007

T cell receptor signaling pathway

 

PN008

TGF-beta signaling pathway

PH009

PN009

Wnt signaling pathway

PH010

PN010


*¸µÅ©¸¦ Ŭ¸¯ÇÏ¸é ¿¢¼¿ÆÄÀÏÀ» ÅëÇØ °¢ Á¦Ç°º° Primer ¸®½ºÆ®¸¦ È®ÀÎÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.