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다양한 형광단백질 리포터를 포함한 Lentiviral expression vectors

형광단백질 리포터 벡터

제조사	제품코드	제품명	용량	가격 (부가세별도)	사용자매뉴얼
Clontech	632152	pLVX-DsRed-Monomer-N1 Vector	10 μg	1,358,000원	,
Clontech	632153	pLVX-DsRed-Monomer-C1 Vector	10 μg	1,358,000원	,
Clontech	632154	pLVX-AcGFP1-N1 Vector	10 μg	1,358,000원	,
Clontech	632155	pLVX-AcGFP1-C1 Vector	10 μg	1,358,000원	,
Clontech	632187	pLVX-IRES-ZsGreen1 Vector	10 μg	1,333,000원	,
Clontech	631237	pLVX-IRES-mCherry Vector	20 ug	1,358,000원	,
Clontech	631238	pLVX-IRES-tdTomato Vector	20 ug	1,358,000원	,
Clontech	632557	pLVX-AmCyan1-C1 Vector	10 ug	1,333,000원	,
Clontech	632558	pLVX-AmCyan1-N1 Vector	10 ug	1,333,000원	,
Clontech	632559	pLVX-DsRed-Express2-C1 Vector	10 ug	1,358,000원	,
Clontech	632560	pLVX-DsRed-Express2-N1 Vector	10 ug	1,358,000원	,
Clontech	632561	pLVX-mCherry-C1 Vector	10 ug	1,358,000원	,
Clontech	632562	pLVX-mCherry-N1 Vector	10 ug	1,358,000원	,
Clontech	632563	pLVX-tdTomato-N1 Vector	10 ug	1,358,000원	,
Clontech	632564	pLVX-tdTomato-C1 Vector	10 ug	1,358,000원	,
Clontech	632565	pLVX-ZsGreen1-N1 Vector	10 ug	1,358,000원	,
Clontech	632566	pLVX-ZsGreen1-C1 Vector	10 ug	1,358,000원	,

Product index

These lentiviral vectors allow you to deliver and stably express your gene of interest as a fluorescent fusion protein in virtually any cell type, including dividing and nondividing cells, stem cells, terminally differentiated cells, and neuronal cells. Three bicistronic lentiviral vectors (pLVX-IRES-mCherry, pLVX-IRES-tdTomato, and pLVX-IRES-ZsGreen1) are also available to allow your gene of interest and a bright fluorescent protein to be coexpressed from a single mRNA transcript (Figure 1).

Clontech’s Living Colors^® DsRed-Monomer, AcGFP1, and mCherry proteins are truly monomeric fluorescent proteins that are ideal for use as fusion tags (Figure 2). ZsGreen1 and tdTomato are supremely bright fluorescent proteins that are excellent reporters for gene expression, or for measuring transduction/transfection efficiency. mCherry is a very popular mutant derived by directed mutagenesis of mRFP1, a monomeric mutant of DsRed. DsRed-Express2 is a rapidly maturing variant of Discosoma sp. red fluorescent protein (DsRed). It is highly soluble and was designed to be better suited for cell and stem cell applications.

All Living Colors proteins are detectable without added cofactors or substrates, making them exceptional, noninvasive tools for live cell studies. For more information on the spectral properties of these proteins click here (pdf).

DsRed-Monomer & AcGFP1

Truly monomeric proteins (see DsRed-Monomer for more information)
Ideal for tagging proteins for trafficking and localization studies
Distinct spectra make them excellent choices for double labeling experiments

ZsGreen1 & tdTomato

The brightest green and red fluorescent proteins-(2.5x and 3.5x brighter than EGFP, respectively)
IRES bicistronic expression for monitoring transduction efficiency
Separate expression of your cDNA and a fluorescent protein from a single transcript

mCherry

A bright monomeric protein
Matures very rapidly-its t_1/2 for maturation at 37°C is approximately 15 minutes

DsRed-Express2

Rapidly maturing red fluorescent protein with enhanced solubility and reduced green emission
Well-suited for cell and stem cell applications

The Lenti-X HT Packaging System & Lenti-X 293T Cell Line

We highly recommended these two products for the production of high-titer lentivirus from all our lentiviral vectors. The Lenti-X HT Packaging System, together with the Lenti-X 293T Cell Line, generates titers that are much higher than those produced by other commercially available systems. Frequently, an entire dish of target cells can be effectively transduced using just 10 μl of unconcentrated viral supernatant. For more details, visit the Lenti-X HT Packaging System product pages.

Applications

Monitor the expression of your lentiviral-delivered genes with fluorescent tags and markers

Storage Conditions

Store all vectors at -20°C

Keyword :

Lentivirus

[개요] Packaging Single Shots

[FAQ] Lentiviral Gene Delivery

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CMV promoter 벡터 시스템

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EF1 α promoter 벡터 시스템

SARS-CoV-2-pseudotyped lentivirus 생산

Lenti-X™ SARS-CoV-2 Packaging Mix

임상 치료제 개발을 위한

3세대 Lentivirus packaging system

다양한 형광단백질 리포터를 포함한 Lentiviral..

형광단백질 리포터 벡터

Tetracycline 존재 하에서만 목적 단백질을 발..

Tet-on inducible Expression (Two vectors)

Tetracycline 존재 하에서만 목적 단백질을 발..

Tet One Inducible Expression (One vector)

Lentivirus를 이용한 sgRNA, Cas9 gene deliver..

Lenti-X™ CRISPR/Cas9 System

Tetracycline 존재 하에서만 목적 단백질을 발..

(Legacy) Tet-Inducible expression 시스템

Pormoterless vector로 pomorter 활성 연구

Reporter assay 시스템 (Promoterless vector)

Transfection 시약을 포함하여 더 간단한 packa..

Packaging single shots

239T, HEK-293 Cell보다 더 높은 packaging 효..

Packaging Cell Lines

1 step qRT-PCR로 역가를 빠르고 정확하게 측정

Lenti-X™ qRT-PCR Titration Kit

p24 ELISA로 빠르고 정확하게 역가 측정

Lenti-X™ p24 Rapid Titer Kit (Single Wash)

스마트폰으로 10분만에 Lentivirus 역가(Titer)..

GoStix™ Plus

Lentivirus 농도가 낮을 때, Mix & Spin으로 간..

Lentivirus 농축 시약

In vivo Lentiviral delivery 적용 시, 필수적..

Lentivirus 정제

Ecotropic lentivirus를 human cell에 적용

Ecotropic Receptor Booster

Lentivirus나 MMLV retrovirus의 더 빠른 trans..

Transduction 시약

목적 세포의 유전자에 삽입된 Provirus copy nu..

Provirus Quantitation Kit

Lentivirus가 목적세포 유전자에 삽입된 위치를..

Integration Site Analysis Kit

최적화된 Transfection Reagent & Packaging Mi..

TransIT® Lentivirus System