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Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > In-Fusion Cloning > [Plus] In-Fusion¢ç EcoDry Cloning

[Plus] In-Fusion¢ç EcoDry Cloning

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
638912
In-Fusion¢ç HD EcoDry Cloning Plus
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
8 ȸ
509,600¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32738, x32739, x33613
Clontech
638913
In-Fusion¢ç HD EcoDry Cloning Plus
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
24 ȸ
1,268,800¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
638914
In-Fusion¢ç HD EcoDry Cloning Plus
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
48 ȸ
2,288,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
638915
In-Fusion¢ç HD EcoDry Cloning Plus
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
96 ȸ
2,906,800¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â


¡Ø In-Fusion Cloning »ý»ý Èı⺸±â

Product Name

Cat. No.

Size

Purification Kit Included

Stellar Competent Cells Included

CloneAmp HiFi PCR Premix Included

In-Fusion HD EcoDry Cloning Plus

638912

8 rxns

Spin Columns

Yes

Yes

638913

24 rxns

Spin Columns

Yes

Yes

638914

48 rxns

Spin Columns

Yes

Yes

638915

96 rxns

Spin Columns

Yes

Yes


Cloning Kits (Lyophilized)-In-Fusion HD Plus EcoDry Cloning System
In-Fusion Cloning SystemÀº ¾î¶² PCR ´ÜÆíÀ̳ª º¹¼öÀÇ DNA ´ÜÆíµµ ¼±ÇüÈ­µÈ vector(linearized vector)¿¡ 15ºÐ(In-Fusion HD EcoDry´Â 30ºÐ)ÀÇ ¹ÝÀÀÀ¸·Î directional cloningÀÌ °¡´ÉÇÏ´Ù (Figure 1). ¶ÇÇÑ PCR ´ÜÆíÀº Á¦ÇÑÈ¿¼Ò󸮳ª phosphorylation°ú °°Àº Ãß°¡ÀûÀº ó¸® ´Ü°è°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù.
In-Fusion È¿¼Ò´Â ¼±ÇüÈ­µÈ vector ¸»´Ü°ú PCR·Î ÁõÆøµÈ PCR ´ÜÆí(insert)ÀÇ ¸»´ÜÀÇ 15bp homologue sequence¸¦ ÀνÄÇÏ¿© À¶ÇÕ½ÃŲ´Ù. ÀÌ 15 bpÀÇ À¶ÇÕ¼­¿­Àº ¿øÇÏ´Â sequence¸¦ ÁõÆøÇϰíÀÚ ÇÏ´Â PCR primer¿¡ µðÀÚÀÎÇÏ¿© ²¸ ³ÖÀ¸¸é ½±°Ô ¸¸µé ¼ö ÀÖ´Ù.

ÀÌ ½ÇÇè¹æ¹ýÀ» »ç¿ëÇÏ¸é ¾Æ·¡¿Í °°Àº ´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÏ´Ù.
  • Subcloning ¾øÀ̵µ º¹¼öÀÇ DNA ´ÜÆíÀ» ¿øÇÏ´Â vector¿¡ Cloning
  • ³»ºÎÀÇ Æ¯Á¤ ºÎºÐ¸¸ ±³Ã¼ÇÏ´Â Modular expression vector Á¦ÀÛ
  • ºÒÇÊ¿äÇÑ ¿°±â°¡ ¾ø´Â Fusion protein ±¸Ãà
  • DNA ´ÜÆíÀÇ Á¦°Å³ª ±³Ã¼
  • InsertÀÇ point mutationÀ̳ª Á¦ÇÑÈ¿¼Ò »çÀÌÆ® ÷°¡
  • À¯ÀüÀÚ³» Çü±¤ ´Ü¹éÁúÀÇ tag ±³Ã¼
  • cDNA·Î UTRs÷°¡



Figure 1. The In-Fusion cloning protocol.



Figure 2. The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).



Figure 3. The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

In-Fusion HD Plus EcoDry Cloning Kit Components
All In-Fusion HD Plus EcoDry Cloning kits contain the In-Fusion HD enzyme premix and reagents for control experiments, in a lyophilized format that provides ease of use and consistency. The In-Fusion premix comes in ready-to-use microtubes that can be stored right on the lab bench until you're ready to perform the In-Fusion reaction. In-Fusion HD Plus EcoDry Cloning kits also include:
  • CloneAmp HiFi High Fidelity PCR Premix, a convenient 2X master mix that provides exceptionally accurate, efficient and fast DNA amplification. The premix contains dNTPs and an optimized buffer, allows rapid set-up of PCR reactions, and facilitates successful cloning.
  • Stellar Competent Cells, an HST08 E. coli strain that provides high transformation efficiency (greater than 5 x 108 cfu/¥ìg) and compliments the efficiency of the In-Fusion HD Cloning Plus kit.
  • NucleoSpin Gel and PCR Clean-Up, for PCR purification and gel extraction.


Keyword : infusion, In-fusion, ÀÎÇ»Á¯, ÀÎÇ»Àü, in fusion, Ŭ·Î´×, cloning, mutagenesis, mutation,soeasy,so easy