´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Ű¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø¼¾ÅÍ 
  • ¾÷¹«¾È³»¦¢ÇмúÁö¿ø
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæÃ»Áö¿ª ÁÖ¹®, ÇмúÁö¿ø
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > In-Fusion Cloning > In-Fusion¢ç Ready Vectors

In-Fusion¢ç Ready Vectors

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
632500
pAcGFP1-C In-Fusion¢ç Ready Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 ¥ìg
616,000¿ø 
770,000¿ø
°¡°ÝÇÒÀÎ
05.02 ~ 06.30
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33232, x32742
Clontech
632501
pAcGFP1-N In-Fusion¢ç Ready Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 ¥ìg
616,000¿ø 
770,000¿ø
°¡°ÝÇÒÀÎ
05.02 ~ 06.30
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33247, x32742
Clontech
632498
pDsRed-Monomer-N In-Fusion¢ç Ready Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 ¥ìg
616,000¿ø 
770,000¿ø
°¡°ÝÇÒÀÎ
05.02 ~ 06.30
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33341, x32742
Clontech
632499
pDsRed-Monomer-C In-Fusion¢ç Ready Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 ¥ìg
616,000¿ø 
770,000¿ø
°¡°ÝÇÒÀÎ
05.02 ~ 06.30
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33332, x32742


pAcGFP1 Vector Information

Restriction map and multiple cloning site (MCS) of pAcGFP1.
Unique restriction sites are in bold. NOTE: The Xba I and Bcl I sites are methylated in the DNA provided by Clontech Laboratories, Inc. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam - host and make fresh DNA.

Location of features
  • lac promoter: 95 - 178
    CAP binding site: 111 - 124
    - 35 region: 143 - 148; - 10 region: 167 - 172
    Transcription start point: 179
    lac operator: 179 - 199
  • lacZ - ECFP fusion protein expressed in E. coli
    Ribosome binding site: 206 - 209
    Start codon (ATG): 217 - 219; stop codon: 1006 - 1008
  • 5' multiple cloning site: 234 - 281
  • Aequorea coerulescens Green fluorescent protein (AcGFP1) gene
    Kozak consensus translation initiation site: 282 - 292
    Start codon (ATG): 289 - 291; stop codon: 1006 - 1008
    Insertion of Val at position 2: 292 - 294
    Last amino acid: 1003 - 1005
  • 3' Multiple Cloning Site: 1009 - 1108 Ampicillin resistance gene:
    Promoter: - 35 region: 1484 - 1450, - 10 region: 1507 - 1512
    Ribosome binding site: 1542 - 1546
    ¥â-lactamase coding sequences:
    start codon (ATG): 1554 - 1556; stop codon: 2412 - 2414
    ¥â-lactamase signal peptide: 1554 - 1622
    ¥â-lactamase mature protein: 1623 - 2411

Keyword :