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ÀüÁ¦Ç°º¸±â
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Cloning °ü·Ã
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In-Fusion Cloning
> In-Fusion¢ç Ready Vectors
In-Fusion¢ç Ready Vectors
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Clontech
632500
pAcGFP1-C In-Fusion¢ç Ready Vector
1 ¥ìg
616,000¿ø
770,000¿ø
05.02 ~ 06.30
,
Clontech
632501
pAcGFP1-N In-Fusion¢ç Ready Vector
1 ¥ìg
616,000¿ø
770,000¿ø
05.02 ~ 06.30
,
Clontech
632498
pDsRed-Monomer-N In-Fusion¢ç Ready Vector
1 ¥ìg
616,000¿ø
770,000¿ø
05.02 ~ 06.30
,
Clontech
632499
pDsRed-Monomer-C In-Fusion¢ç Ready Vector
1 ¥ìg
616,000¿ø
770,000¿ø
05.02 ~ 06.30
,
Product index
½Ä¹°¿ë Vectors
Takara Plant ½ÇÇè °ü·Ã..
Agrobacterium tumefaci..
pRI 101 DNA ½Ã¸®Áî
pRI 201 DNA ½Ã¸®Áî
pRI 909/910 DNA
È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
pAUR101 DNA
pAUR112 DNA
pAUR123 DNA
pAUR135 DNA
pAUR224 DNA
pAUR316 DNA
pPTR I DNA
pPTR II DNA
Genomic DNA
Genomic DNA
Thermus thermophilus H..
¥ëDNA
¥õX174 RF I DNA
DNA Ligation
Cloning ½ÇÇè ÀÔ¹®ÀÚ¸¦ ..
Takara Cloning FAQ
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½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Fragmentation Kit
Kilo-Sequence ¿ë Delet..
Mighty Cloning Reagent..
Mighty TA-cloning Kit
Mighty TA-cloning Reag..
TaKaRa LA PCR¢â in vit..
TaKaRa T-Vector pMD20
In-Fusion Cloning
[Overview] In-Fusion¢ç..
[°³¿ä] So Easy Product
Online Tools for In-Fu..
[Àû¿ë] In-Fusion¢ç Clo..
[FAQ] In-Fusion¢ç Clon..
[Tip] In-Fusion¢ç Clon..
½ÇÁ¦ °í°´ÀÇ »ç¿ëÈıâ
2ź ½ÇÁ¦ »ç¿ëÀÚ Èıâ
[NEW] In-Fusion¢ç Snap..
[NEW] µ¿°á°ÇÁ¶ In-Fusi..
[Kit] In-Fusion¢ç Clon..
[Kit] In-Fusion¢ç EcoD..
[Plus] In-Fusion¢ç Clo..
[Plus] In-Fusion¢ç Eco..
In-Fusion¢ç Ready Vect..
Genomic DNA ÁõÆø¿ë Kit
GenomeWalkerKit
Vector (Cloning ¿ë)
M13 mp18 Single Strand..
pBR322 DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pUC118/119 type vector
pUC18/19 type vector
TaKaRa T-Vector pMD20
Competent Cells & Electro-..
Takara Competent Cell ..
Agrobacterium tumefaci..
Chaperone Competent Ce..
E. coli Competent Cell..
E. coli Competent Cell..
E. coli Electro-Cells ..
¼ö½ÄÈ¿¼Ò¿¡ ´ëÇÏ¿©
¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
¼ö½ÄÈ¿¼Òº° ¿ëµµ
¼ö½ÄÈ¿¼Ò£¯Ligase
E. coli DNA Ligase
T4 DNA Ligase
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Alkaline Phosphatase (..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
T4 Polynucleotide Kina..
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DNA Polymerase I (E. ..
Klenow Fragment (Large..
Poly(A) Polymerase
Pyrophosphatase (inorg..
SP6 RNA Polymerase
T4 DNA Polymerase
T7 RNA Polymerase
T7 RNA Polymerase ver...
Takara IVTpro¢â mRNA S..
Terminal Deoxynucleoti..
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BAL 31 Nuclease
DNA Topoisomerase I
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S1 Nuclease
Sequencing Primer
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Deoxyribonucleoside 8..
dNTP Mixture
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¹èÁö ¡¤ Buffer (ºÐ¸»)
AccuGENE 20¡¿SSPE Buff..
Bovine Serum Albumin S..
IPTG£¨dioxane free£©£¨..
X-Gal£¨5-Bromo-4-Chlor..
±× ¿Ü (À¯Àü°øÇÐ °ü·Ã)
DNA-OFF¢ç
Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase (°è»ó±Õ ¼¼Æ÷..
pAcGFP1 Vector Information
Restriction map and multiple cloning site (MCS) of pAcGFP1.
Unique restriction sites are in bold. NOTE: The Xba I and Bcl I sites are methylated in the DNA provided by Clontech Laboratories, Inc. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam - host and make fresh DNA.
Location of features
lac promoter: 95 - 178
CAP binding site: 111 - 124
- 35 region: 143 - 148; - 10 region: 167 - 172
Transcription start point: 179
lac operator: 179 - 199
lacZ - ECFP fusion protein expressed in E. coli
Ribosome binding site: 206 - 209
Start codon (ATG): 217 - 219; stop codon: 1006 - 1008
5' multiple cloning site: 234 - 281
Aequorea coerulescens Green fluorescent protein (AcGFP1) gene
Kozak consensus translation initiation site: 282 - 292
Start codon (ATG): 289 - 291; stop codon: 1006 - 1008
Insertion of Val at position 2: 292 - 294
Last amino acid: 1003 - 1005
3' Multiple Cloning Site: 1009 - 1108 Ampicillin resistance gene:
Promoter: - 35 region: 1484 - 1450, - 10 region: 1507 - 1512
Ribosome binding site: 1542 - 1546
¥â-lactamase coding sequences:
start codon (ATG): 1554 - 1556; stop codon: 2412 - 2414
¥â-lactamase signal peptide: 1554 - 1622
¥â-lactamase mature protein: 1623 - 2411
Keyword :
In-Fusion Cloning
[Overview] In-Fusion¢ç Cloning
[°³¿ä] So Easy Product
Online Tools for In-Fusion
[Àû¿ë] In-Fusion¢ç Cloning Àû¿ë»ç·Ê
[FAQ] In-Fusion¢ç Cloning
[Tip] In-Fusion¢ç Cloning
½ÇÁ¦ °í°´ÀÇ »ç¿ëÈıâ
2ź ½ÇÁ¦ »ç¿ëÀÚ Èıâ
ÃÖ°íÀÇ Colony Çü¼º´É°ú Á¤È®ÇÑ PCR cloning
[NEW] In-Fusion¢ç Snap Assembly
½Ç¿Â º¸°ü Master mix
[NEW] µ¿°á°ÇÁ¶ In-Fusion¢ç Snap Assembly
PremixÈ! ½Ã°£´ÜÃà! È¿À²¼º Çâ»ó!
[Kit] In-Fusion¢ç Cloning
[Kit] In-Fusion¢ç EcoDry Cloning
[Plus] In-Fusion¢ç Cloning
[Plus] In-Fusion¢ç EcoDry Cloning
In-Fusion¢ç Ready Vectors