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Home > ÀüÁ¦Ç°º¸±â > ±â±â ¡¤ ¼Ò¸ðÇ° > Single Cell ºÐ¼®ÀåÄ¡ > Single-cell Full-Length transcriptome analysis

Single-cell Full-Length transcriptome analysis

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Takara
640202
SMART-Seq¢ç ICELL8¢ç Reagent Kit
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640203
SMART-Seq¢ç ICELL8¢ç Reagent Kit
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640204
SMART-Seq¢ç ICELL8¢ç Reagent Kit
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640222
SMART-Seq¢ç ICELL8¢ç cx Application Kit - 1 Chip
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SMART-Seq¢ç ICELL8¢ç cx Application Kit - 3 Chip
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640224
SMART-Seq¢ç ICELL8¢ç cx Application Kit - 5 Chip
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640205
SMART-Seq¢ç ICELL8¢ç Indexing Primer Set - A
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1 chip
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640218
SMART-Seq¢ç ICELL8¢ç Indexing Primer Set - B
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1 chip
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640019
ICELL8¢ç 350v Chip
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640212
ICELL8¢ç Collection Kit - L
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640206
ICELL8¢ç Loading Kit - B
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640018
MSND 384-Well Source Plate and Seals (430-000025)
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  • High-throughput single-cell analysis-generate sequencing data from >1,000 single cells in parallel
  • Multiplex for higher throughput-use SMART-Seq ICELL8 indexing primer sets A and B on two chips, and then pool the samples to allow sequencing of >2,000 cells simultaneously
  • Sample flexibility-analyze cells from up to eight different samples in a single experiment
  • Reduced sample handling-minimize variability with automated cell isolation and selection, and sample pooling prior to sequencing-ready library construction
  • Illumina sequencing libraries-generate libraries suitable for sequencing on all Illumina platforms

ICELL8 cx Single Cell SystemÀ¸·Î ºÐ¸®µÈ single cellÀº ºó 5,184 nano wellÀÇ 350V chip(Code 640019)À¸·Î ºÐÁֵǰí, CellSelect Software¸¦ »ç¿ëÇÏ¿© single cellÀÌ ºÐÁֵǾî ÀÖ´Â wellÀ» ½Äº°, ¼±ÅÃÇÑ ÈÄ SMART RT-PCR ½Ã¾àÀ» ¼±ÅÃÇÑ well¿¡ Ãß°¡·Î ºÐÁÖÇÑ´Ù. single cell°ú RT-PCR ½Ã¾àÀÌ ºÐÁÖµÈ chipÀº ICELL8 cx Thermal cycler·Î ¿Å°Ü¼­ 1st strand cDNAÀÇ ÇÕ¼º°ú ÁõÆøÀ» À§ÇØ one-step RT-PCRÀÌ ÀÌ·ïÁö°Ô µÈ´Ù. ÀÌ·¸°Ô ÁغñµÈ full-length cDNA´Â TDE1(Illumina Tagment DNA Enzyme)¿¡ ÀÇÇØ tag°¡ ÁöÁ¤µÇ°í, ÀÌÈÄ forward ¹× reverse indexing primer(Code 640205 ¹× 640218)¸¦ »ç¿ëÇÏ¿© ÁõÆøµÈ´Ù. ÀÌ¿Í °°ÀÌ Á¦ÀÛµÈ library¸¦ ȸ¼ö, Á¤Á¦ ¹× ÁõÆøÇÏ¿© ÃÖÁ¾ sequencingÀÌ °¡´ÉÇÑ library¸¦ ÁغñÇÒ ¼ö ÀÖ´Ù.

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