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Universal Genomic DNA Extraction Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
9765A
TaKaRa MiniBEST Universal Genomic DNA Extraction Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
(Package)
150,400¿ø 
188,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
9765_e.v1306Da.pdf
Takara
9765B
TaKaRa MiniBEST Universal Genomic DNA Extraction Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (9765A x 4)
672,000¿ø 


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TaKaRa MiniBEST Universal Genomic DNA Extraction KitÀº Ç÷¾×, ±×¶÷À½¼º¹ÚÅ׸®¾Æ, Æ÷À¯·ù ¹è¾ç¼¼Æ÷, ½Ä¹° Á¶Á÷À̳ª µ¿¹° Á¶Á÷°ú °°Àº ´Ù¾çÇÑ ½Ã·á·ÎºÎÅÍ genomic DNA¸¦ Á¤Á¦Çϱâ À§ÇÑ Á¦Ç°ÀÌ´Ù.
º» kitÀº »ý¹°½Ã·á·ÎºÎÅÍ È¿°úÀûÀ¸·Î genomic DNA¸¦ Á¤Á¦ÇÒ ¼ö ÀÖµµ·Ï Spin Column membrane°ú Àü¸Å lysis buffer¸¦ ä¿ëÇÏ°í ÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇÏ¸é ¿ëÇØµÈ Á¶Á÷ ¼¼Æ÷·ÎºÎÅÍ 20ºÐÀ̳» Àü °úÁ¤À» ¼öÇàÇÏ¿© °í¼øµµÀÇ genomic DNA¸¦ ½Å¼ÓÇÏ°Ô ºÐ¸®ÇÒ ¼ö ÀÖ´Ù.
º» kitÀ» ÀÌ¿ëÇϸé 50~200 ¥ìlÀÇ Æ÷À¯·ù ÀüÇ÷(Ç÷¾×ÀÀ°í ¹æÁöÁ¦ ÇÔÀ¯), 1~10 ¥ìlÀÇ ÀüÇ÷³»(Ç÷¾×ÀÀ°í ¹æÁöÁ¦ÇÔÀ¯) À¯ÇÙÀûÇ÷±¸, 1.0~5.0 x 109 ±×¶÷À½¼º ¹ÚÅ׸®¾Æ, 1.0 x 105~1.0 x 107 ¹è¾ç¼¼Æ÷, 2~30 mgÀÇ Æ÷À¯·ù Á¶Á÷À̳ª 25~100 mgÀÇ ½Ä¹°Á¶Á÷À¸·ÎºÎÅÍ °í¼øµµÀÇ genomicDNA¸¦ 10 ¥ìgÁ¤µµ ÃßÃâÇÒ ¼ö ÀÖ´Ù. º» kitÀ¸·Î ÃßÃâÇÑ genomic DNA´Â PCR, Southern blotting, RAPD, AFLP, RFLP³ª ±× ¿Ü ´Ù¾çÇÑ ºÐÀÚ»ý¹°ÇÐ ½ÇÇè¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.

³»¿ë
º» KitÀº Reagent Set¿Í Column SetÀ¸·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
Reagent Set

Proteinase K (20 mg/ml)

1ml

RNase A (10 mg/ml)

0.5 ml

Buffer GL *1

12 ml

Buffer GB *1

12 ml

Buffer WA *1

28 ml

Buffer WB *2

24 ml

Elution Buffer

14 ml


£ª1: Contain strong denaturant. Be careful to avoid contacting with eyes and skin.
      In the case of such contact, wash immediately with plenty water and seek medical advice.
£ª2: Before the first use of the kit, add 56 ml of 100% ethanol to Buffer. Mix well.
Column Set

Spin Columns

50

Collection tubes

50


¡Ø ÀÌÇÏ ½Ã¾àÀº º°µµ Áغñ°¡ ÇÊ¿äÇÕ´Ï´Ù.
1. 100% Ethanol
2. Sterilized water or Tris-HCl (pH8.0)
3. PBS
º¸°ü ¹× ¿î¼Û
1. The kit can be stored at room temperature (15 - 25¡É).
2. Proteinase K can be stored at room temperature (15 - 25¡É) for 6 months, and should be stored at -20¡É for long-term.
3. RNase A (10 mg/ml) can be stored at room temperature (15 - 25¡É) for 6 months, and should be stored at -20¡É for long-term.
4. The kit is shipped at room temperature (15 - 25¡É).

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