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Overview - All about AAV

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´ÙÄ«¶ó¿¡¼­´Â AAV¸¦ helper virus¾øÀÌ ¾ÈÀüÇÏ°Ô Á¦ÀÛÇϱâ À§ÇÑ AAVpro¢ç Helper Free System°ú ÇÔ²² AAVpro¢ç CRISPR/Cas9, Tet-One ½Ã½ºÅÛ µî ´Ù¾çÇÑ AAV ½Ã½ºÅÛÀ» Á¦°øÇÏ°í ÀÖ´Ù. ¶ÇÇÑ, AAVÀÇ ¿ª°¡ Çâ»óÀ» À§ÇÑ Àü¿ë transfection ½Ã¾à°ú packaging cell line, packaging cell lineÀ¸·ÎºÎÅÍ AAV¸¦ È¿À²ÀûÀ¸·Î ÃßÃâÇϱâ À§ÇÑ AAVpro¢ç Extraction Solution, °í¼øµµ AAV Á¤Á¦¸¦ À§ÇÑ AAVpro¢ç Purification Kit (All serotype, AAV2), ºü¸£°í °£ÆíÇÏ°Ô AAVÀÇ ¿ª°¡ ÃøÁ¤À» À§ÇÑ Real Time PCR ¹æ½ÄÀÇ AAVpro¢ç Titration Kit µî AAV ½ÇÇè¿¡ ÇÊ¿äÇÑ ´Ù¾çÇÑ Á¦Ç°À» °ø±ÞÇÏ°í ÀÖ´Ù.
ÀÌ·¯ÇÑ ´ÙÄ«¶óÀÇ AAVpro¢ç Á¦Ç°À» ÀÌ¿ëÇϸé, È¿À²ÀûÀÎ transductionÀ» À§ÇÑ °í¿ª°¡ÀÇ AAV¸¦ »ý»ê, ÃßÃâ, Á¤Á¦ ¹× titration ÇÏ¿©, µ¿¹° ¼¼Æ÷³ª °¢°¢ÀÇ µ¿¹° °³Ã¼¿¡ À¯ÀüÀÚ µµÀÔ (in vivo transduction)ÇÒ ¼ö ÀÖ´Ù.

[±×¸² 1] AAV workflow



  0. Overview

AAV (Adeno-associated virus)¶õ?


AAVÀÇ ±¸Á¶Àû Ư¡


Serotype¿¡ µû¸¥ AAVÀÇ Á¶Á÷ °¨¿° ƯÀ̼º

  1. Virus packaging

AAV packaging system


AAV packaging cell line


Transfection reagent for AAV packaging


  2. Virus extraction and purification

Virus extraction and purification


Virus concentration


  3. Virus titration

AAVpro¢ç Titration Kit (for Real Time PCR) Ver2. (Code 6233)
  • AAV2 À¯·¡ÀÇ ITR (Inverted Terminal Repeat)¸¦ ÀÌ¿ëÇØ, AAV ¿ª°¡ ÃøÁ¤
    (** ´ëºÎºÐÀÇ AAV ½Ã½ºÅÛÀº serotype¿¡ °ü°è¾øÀÌ AAV2 À¯·¡ ITR ¼­¿­À» È°¿ëÇÏ´Â °æ¿ì°¡ ¸¹À½)
  • ±âÁ¸ÀÇ DNA blot ¶Ç´Â ELISA ¹æ¹ýº¸´Ù Á¤È®ÇÑ Á¤·®