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Home > 전제품보기 > Transfection 시약 > siRNA Transfection 시약 > TransIT-TKO Transfection Reagent
siRNA 도입 용 시약

TransIT-TKO Transfection Reagent

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제조사 제품코드 제품명 용량 가격
(부가세별도)		 비고 사용자매뉴얼
Mirus
 MIR 2154
 TransIT-TKO Transfection Reagent
관련학술 구매하기 		0.4 ml
 가격문의   ml015_transit_tko_transfection_reagent.pdf
Mirus
 MIR 2150
 TransIT-TKO Transfection Reagent
관련학술 구매하기 샘플신청  		1.5 ml
 가격문의  
Mirus
 MIR 2155
 TransIT-TKO Transfection Reagent
관련학술 구매하기 		1.5 ml×5
 가격문의  
Mirus
 MIR 2156
 TransIT-TKO Transfection Reagent
관련학술 구매하기 		1.5 ml×10
 가격문의  

  • 광범위한 세포에 siRNA를 고효율로 도입 가능
  • 낮은 세포 독성으로 배지교환 불필요
  • 높은 Knock-down 효율로, 재현성 높은 실험 결과

Technical Report: High Efficiency siRNA Delivery In Vitro (pdf) (클릭)
Publication: Delivery of small interfering RNA to mammalian cells in culture (클릭)
제품설명
TransIT-TKO는 짧은 dsRNA (siRNA)을 진핵세포에 transfection하기 위해서 최적화된 시약이다. 세포에 대한 독성이 낮고, 높은 효율로 siRNA를 도입할 수 있으므로 표적 유전자의 발현을 Knock-down 하는 실험에 사용할 수 있다. 또한 혈청을 포함하는 배지에도 transfection이 가능하며, 배지교환이 불필요하므로 시간을 절약할 수 있다.
24 well plate의 경우 본 시약 1 ml으로 약 500회분의 transfection을 실시할 수 있다. 그리고 TransIT-LT1 (Code MIR 2304/MIR 2300)과 함께 이용하면 siRNA 와 plasmid DNA의 co-transfection이 가능하다.


Figure 1. High Efficiency Knockdown and Low Toxicity Using TransIT-TKO® Reagent in HeLa Cells.  Firefly and sea pansy luciferase reporter vectors were co-transfected into HeLa cells using TransIT®-LT1 Reagent. Cells were incubated for at least 4 hours, split into 24-well plates, allowed to adhere and transfected with 25 nM of either a non-targeting siRNA or an anti-firefly luciferase siRNA using the indicated reagents with the volumes noted beneath each well. Luciferase expression, normalized to non-targeting siRNA control (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph.


Figure 2. Delivery of Fluorescently-Labeled siRNA Using TransIT-TKO® Transfection Reagent.  HeLa (70% confluence) cells in 12-well plates were transfected with TransIT-TKO® Transfection Reagent (3 μl/well) and Label IT® siRNA Tracker Fluorescein-labeled siRNA duplexes (GREEN, 50 nM final concentration in the well). The cells were incubated 24 hours post-transfection then fixed and counterstained with TO-PRO®-3 (nuclei, BLUE) (Life Technologies) and Alexa Fluor® 546 Phalloidin (actin, RED) (Life Technologies). Confocal images were acquired on a Zeiss LSM 510 Confocal Microscope.

표 1. TransIT-TKO를 이용한 목적 유전자 Knock-down 효율
세포

Target Gene

Knock-Down efficiency
(qRT-PCR)

BNL CL.2 (mouse liver)

MAPK1

80 %

MAPK3

83 %

HeLa (human cervix)

Lamin A / C

80 %

GAPDH

80 %

Hepa1c1c7 (mouse liver)

MAPK1

80 %

MAPK3

75 %

MEK1

75 %

PTEN

80 %

HepG2 (human liver)

MAPK1

80 %

NIH 3T3-L1

MAPK1

70 %

MAPK3

70 %

Secondary Human Astrocytes

Lamin A / C

80 %

Primary Mouse Hepatocytes

ABC A1

70 %

Lamin A / C

81 %
보존 4℃

Keyword : V2154,MIR2154,siRNA,V2150,MIR2150,V2155,MIR2155,V2156,MIR2156
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