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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > [Legacy] NGS Products > (Legacy) Human TCR a/b Profiling Kit
TCR ¥á/¥â V(D)J °¡º¯¿µ¿ªÀÇ Full-length sequence °¡´É

(Legacy) Human TCR a/b Profiling Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
µ¥ÀÌÅÍ°¡ ¾ø½À´Ï´Ù

* º» Á¦Ç°Àº ÃֽŹöÀüÀÎ SMART-Seq¢ç Human TCR (with UMIs) (Code 634779-634781)·Î ¾÷±×·¹ÀÌµå µÇ¾ú½À´Ï´Ù.
* ÇϳªÀÇ ¿¬±¸ ÇÁ·ÎÁ§Æ®¿¡´Â µ¿ÀÏÇÑ ¹öÀüÀÇ Á¦Ç°À» »ç¿ëÇϽô °ÍÀ» ±ÇÀåµå¸³´Ï´Ù.


  • TCR-alpha, TCR-beta chainÀ» µ¿½Ã¿¡ ¶Ç´Â °³º°ÀûÀ¸·Î NGS Library Á¦ÀÛ °¡´É
  • Complete V(D)J sequence information: T-cell receptor (TCR)ÀÇ V(D)J °¡º¯¿µ¿ª (V(D)J variable regionÀÇ Full-length sequencing
  • Flexible sample inputs: ¼Ò·®ÀÇ Human blood RNA sample (10ng - 3ug) ¶Ç´Â intact lymphocytes (50 - 10,000 purified T cells)¿¡¼­ NGS Library¸¦ Á¦ÀÛ °¡´É
  • Illumina-ready sequencing libraries: ligation-independent ¹æ½ÄÀ¸·Î illumine adaptor ¹× index sequence ºÎÂø °¡´É
  • Exceptional sensitivity, and excellent reproducibility
Á¦Ç°¼³¸í
SMARTer Human TCR a/b Profiling KitÀº T-cell receptor (TCR) repertoireÀÇ NGS ºÐ¼®À» À§ÇÑ Illumina platform NGS Library Á¦ÀÛ Å°Æ®ÀÌ´Ù. º» Á¦Ç°Àº ¹Ì·®ÀÇ Human blood RNA sample (10ng - 3ug), ¶Ç´Â intact lymphocytes (50 - 10,000 purified T cells)·ÎºÎÅÍ TCR Àü»çüÀÇ V(D)J °¡º¯¿µ¿ª (variable region) ºÐ¼®À» À§ÇÏ¿© 5¡¯ RACE À¯»ç ±â¼úÀ» Á¢¸ñÇÏ¿´À¸¸ç, TCR mRNAÀÇ Full length sequence¸¦ ºÐ¼®ÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇϸé TCR-¥á, TCR-¥â chainÀ» µ¿½Ã¿¡ ¶Ç´Â chain¿¡ ´ëÇÑ ¶óÀ̺귯¸®¸¦ Á¦ÀÛÇÏ¿© ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.
Multiplexing PCRÀ» ÀÌ¿ëÇÏ´Â ±âÁ¸ÀÇ TCR profiling ¹æ¹ý°ú ´Þ¸®, º» Á¦Ç°Àº °¢°¢ÀÇ TCR-¥á¿Í TCR-¥â chain¿¡ ´ëÇÑ single primer¸¦ »ç¿ëÇÏ´Â 2´Ü°èÀÇ PCR ¹æ¹ýÀ¸·Î Illumina¿ë ¶óÀ̺귯¸®¸¦ ÁõÆøÇÑ´Ù.
º» Á¦Ç°Àº LNA ±â¼úÀÌ °áÇÕµÈ SMART (Switching Mechanism at 5¡¯ End of RNA Template)¹ýÀ» ÀÌ¿ëÇϹǷΠ¹Î°¨µµ°¡ ¸Å¿ì ³ô°í ÆíÇ⼺ÀÌ ÀûÀº TCR mRNA sequence ºÐ¼®À» ÇÒ ¼ö ÀÖ´Ù.


±×¸² 1. Protocol overview and timeline




±×¸² 2. SMARTer TCR profiling ±â¼úÀÇ Library Á¦ÀÛ °úÁ¤ ¹× ¿ø¸®
Panel A. TCR subunit mRNAÀÇ ¿ªÀü»ç¹ÝÀÀ°ú PCR ÁõÆø °úÁ¤
Panel B. TCR-¥á, TCR-¥â chainÀÇ ÁõÆøÀ» À§ÇÑ semi-nested PCR


±×¸² 3. TCR sequencing libraryÀÇ Electropherogram profiles
Panel A. Typical Fragment Analyzer profile of sequencing library for TCR-¥á and TCR-¥â, obtained from peripheral blood leukocyte RNA Panel B. Typical Bioanalyzer profile of sequencing library for TCR-¥á and TCR-¥â, obtained from peripheral blood leukocyte RNA (same library as Panel A) Panel C. Typical Fragment Analyzer profile of sequencing library for TCR-¥á and TCR-¥â, obtained from monoclonal Jurkat T-cell RNA. Panel D. Typical Bioanalyzer profile of sequencing library for TCR-¥á and TCR-¥â, obtained from Jurkat T-cell RNA (same library as Panel C)
Application
Human TCR repertoire analysis (TCR-alpha and TCR-beta subunits)
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
SMARTer Human TCR a/b Profiling Kit (Code 635014)
  • SMARTer Human TCR a/b Profiling Kit Components (Cat. No. 635017; Not sold separately)
  • TCR a/b Human Indexing Primer Set HT for Illumina - 12 (Cat. No. 635020; Not sold separately)
  • SeqAmp DNA Polymerase (Cat. No. 638504)

Keyword : TCR,CDR3,T cell receptor,repertoire,variable region,Immune cell,Immune-seq,Immunology,,SMART,smart-seq,NGS,Next generation sequencing,cancer,Cancer research,¾Ï¿¬±¸,vaccine,¹é½Å,¹é½Å¿¬±¸

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