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NGS °ü·Ã
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NGS ºÐ¼®¿ë PCR È¿¼Ò
> PreAmp Master Mix
100pg DNA·ÎºÎÅÍ ÆíÇ⼺ ¾ø´Â Preamplification (for qPCR, NGS, genotyping)
PreAmp Master Mix
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Clontech
638541
Prelude¢â PreAmp Master Mix
40ȸ (638542 x 4)
769,600¿ø
962,000¿ø
11.01 ~ 12.27
Clontech
638542
Prelude¢â PreAmp Master Mix
10ȸ
Á¾¸ÅÀÏ :2024.10.28
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
mRNA-Seq (Single cell)
[New]Single cell mRNA-..
[New]Ultra low input m..
[New]High throughput, ..
Ultra low input mRNA-S..
High throughput, Autom..
Ultra low input 3¡¯end..
Stranded RNA-seq
[New](+UMI) Pico i..
[New]Pico input strand..
[New]Single cell stran..
[New]Mid Input Strande..
[New]High Input Strand..
Total RNA-Seq Kit (FFP..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
Single cell Stranded R..
Mid Input Stranded RNA..
High Input Stranded RN..
Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[New]UMI Àû¿ë Human TC..
[New]UMI Àû¿ë Human BC..
[New]UMI Àû¿ë Mouse TC..
[New]UMI Àû¿ë Mouse BC..
UMI Àû¿ë Human TCR pro..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) PicoPLEX¢ç WG..
Least-biased preamplification
- Ÿ»çÀÇ preamplification Á¦Ç°¿¡ ºñÇÏ¿© ÀÌ»óÁ¡ (outlier)ÀÌ 2 ~ 3¹è ´õ ÀûÀº ÆíÇ⼺ÀÌ ¸Å¿ì ³·Àº ÁõÆø °¡´É
Greatest sensitivity
- ÃÖ¼Ò 100pg cDNA ¶Ç´Â gDNA·ÎºÎÅÍ ÁõÆø
Universal compatibility
- ÁõÆøµÈ »ùÇà (preamplified sample)Àº Real Time PCR (100°³ÀÇ intercalating dye¹ý, ¶Ç´Â dual-labeled probe¹ý), SNP genotyping ¶Ç´Â target enriched NGS¿¡ Àû¿ë
Convenient, ready-to-use master mix -
Master mix ŸÀÔÀ¸·Î cDNA¿Í gDNA »ùÇÃ, Primer set Ãß°¡ ÈÄ PCR ¹ÝÀÀ (10 - 14 Cycles)
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Prelude¢â PreAmp Master Mix´Â 2X PCR mix ÇüÅÂÀÇ Á¦Ç°À¸·Î, ¾ÆÁÖ ÀûÀº ¾çÀÇ cDNA ¶Ç´Â gDNA (100pg - 10ng)·ÎºÎÅÍ 100°³ ÀÌ»óÀÇ Å¸°Ù¿¡ ´ëÇØ ÆíÇ⼺ ¾øÀÌ preamplificationÀÌ °¡´ÉÇÑ PCR È¿¼ÒÀÌ´Ù. º» Á¦Ç°À¸·Î ÁõÆøµÈ »ê¹°Àº Real Time PCR (qPCR), Targeted enrichment, ¶Ç´Â genotyping ¸ñÀûÀ¸·Î Àû¿ëÇÒ ¼ö ÀÖ´Ù.
Preamplification ½ÇÇèµ¥ÀÌÅÍ
PrimerArray Cell Cycle (Human) panel¿¡ Æ÷ÇÔµÈ 96°³ÀÇ Å¸°Ù À¯ÀüÀÚ¿¡ ´ëÇØ Takara PreAmp Master Mix·Î preamplificationÇÑ °á°ú¸¦ ³ªÅ¸³»¾ú´Ù. ƯÁ¤ À¯ÀüÀÚ¿¡ ´ëÇؼ¸¸ xÃà¿¡ ¸í¸íÇÏ¿´À¸¸ç, Àüü Ÿ°Ù À¯ÀüÀÚ ¸®½ºÆ®´Â
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¿¡¼ È®ÀÎ °¡´ÉÇÏ´Ù. Ct°ªÀÌ 32¸¦ ÃÊ°úÇÏ´Â nompreamplified gene¿¡ ´ëÇؼ´Â Ç¥±âµÇÁö ¾Ê¾Ò´Ù (±×¸² 1 - ±×¸² 6).
±×¸² 1. The benefits of preamplification.
±×¸² 2. Uniform representation following preamplification.
±×¸² 3. Uniformity following either 10 or 14 cycles of preamplification.
±×¸² 4. Delta-Delta Ct analysis demonstrates unbiased preamplification between 10 and 14 cycles.
±×¸² 5. Sensitive, unbiased preamplification using as little as 12.5 pg of starting material
±×¸² 6. Prelude¢â PreAmp Master Mix provides less biased preamplification than other master mixes
FFPE »ùÇ÷κÎÅÍ 162°³ÀÇ À¯¹æ¾Ï À¯ÀüÀÚ¿¡ ´ëÇØ Prelude¢â PreAmp Master Mix¿Í TaqMan¢â PreAmp Master Mix·Î preamplification ÈÄ ºÐ¼®ÇÑ °á°ú, Prelude¢â PreAmp Master Mix°¡ º¸´Ù ÆíÇ⼺ÀÌ ÀûÀº ºÐ¼® °á°ú¸¦ º¸¿´´Ù (±×¸² 7).
±×¸² 7. Prelude¢â PreAmp Master Mix provides highly unbiased preamplification when assaying 162 breast cancer targets in FFPE tissue.
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶ ¹Ù¶ø´Ï´Ù)
Prelude¢â PreAmp Master Mix (Code 638541) 4 x 250 §¡
Prelude¢â PreAmp Master Mix (Code 638542) 1 x 250 §¡
º¸Á¸ - 20¡ÆC
Keyword : Preamplification,Preamp,gene expression,profiling,real time pcr,qpcr,Genotyping
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymerase
PrimeSTAR¢ç GXL DNA Polymerase
100pg DNA·ÎºÎÅÍ ÆíÇ⼺ ¾ø´Â Preamplification..
PreAmp Master Mix