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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > High input DNA-seq > ThruPLEX¢ç Tag-Seq HV
UMI°¡ µµÀÔµÈ FFPE, cell-free DNA-seq ºÐ¼® Á¦Ç°

ThruPLEX¢ç Tag-Seq HV

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Clontech
R400784
ThruPLEX¢ç Tag-Seq HV PLUS Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24 ȸ
2,149,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX Tag-Seq HV PLUS User Manual-020821.pdf
Clontech
R400785
ThruPLEX¢ç Tag-Seq HV PLUS Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ
7,459,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R400742
ThruPLEX¢ç Tag-Seq HV
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24 ȸ
1,923,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX%20Tag-Seq%20HV%20User%20Manual_022720.pdf
Clontech
R400743
ThruPLEX¢ç Tag-Seq HV
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ
6,641,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R400739
ThruPLEX¢ç HV UDI 1-24
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 ȸ
518,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX HV UDI Protocol-at-a-Glance_022720.pdf
Clontech
R400738
ThruPLEX¢ç HV UDI Set A
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 ȸ
1,855,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX HV UDI Protocol-at-a-Glance_022720.pdf

* ThruPLEX¢ç Tag-Seq HV PLUS Kit ³»¿¡´Â enzymatic fragmentation moduleÀ» Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù.
* Á¦Ç° ³»¿¡´Â HV Àü¿ë UDI°¡ Æ÷ÇԵǾî ÀÖÀ¸¸ç, Ãß°¡·Î ÇÊ¿ä½Ã¿¡¸¸ ±¸¸Å ¹Ù¶ø´Ï´Ù. HV Àü¿ëÀÌ ¾Æ´Ñ index´Â ȣȯµÇÁö ¾ÊÀ¸´Ï, Âü°í ¹Ù¶ø´Ï´Ù.


  • »ùÇÃÀ» ³óÃàÇÒ ÇÊ¿ä ¾øÀÌ single-tube workflow·Î Illumina¢ç NGS library Á¦ÀÛ
  • Enzymatic fragmentation module Æ÷ÇÔ (300 bp, 450 bp ¼±Åà °¡´É)
  • FFPE, cfDNA 5 ~200 ng ·ÎºÎÅÍ °íÇ°ÁúÀÇ µ¥ÀÌÅÍ È¹µæ
  • High-throughput sequencer (e.g. NovaSeq¢â)À» Æ÷ÇÔÇÑ ¸ðµç ±â±â¿¡¼­ ºÐ¼® °¡´ÉÇÑ ³ôÀº ¼öÀ²ÀÇ library ȹµæ
  • ³ôÀº GC ºÎÀ§¿¡¼­µµ bias ¾øÀÌ Çâ»óµÈ ¼º´É
  • Incorporation of discrete, balanced molecular tags (UMIs) in ThruPLEX Tag-Seq HV
  • Low-frequency º¯À̸¦ ÀçÇö¼º°ú ¹Î°¨µµ ³ô°Ô NGS·Î ºÐ¼®
  • Amplification°ú sequencing ¿À·ù ÃÖ¼ÒÈ­

Á¦Ç° ¼³¸í
ThruPLEX¢ç Tag-Seq HV´Â 96 indexed library¸¦ ÀÌ¿ëÇØ Illumina¢ç NGS library¸¦ ÀÌ¿ëÇÑ multiplex ºÐ¼®À» À§ÇØ Á¦À۵Ǿú´Ù. ThruPLEX¢ç Tag-Seq HV´Â ÃÖ´ë 30 §¡ÀÇ »ùÇÃÀ» ÀÌ¿ëÇØ ³ôÀº ´Ù¾ç¼º°ú GC representationÀ» º¸À¯ÇÑ DNA library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖµµ·Ï ÃÖÀûÈ­ µÇ¾ú´Ù. Library Á¦ÀÛÀ» À§ÇØ, 5 - 200 ngÀÇ fragmentated double-stranded DNA°¡ Àû¿ëµÉ ¼ö ÀÖ´Ù. ½ÇÇè Àü °úÁ¤Àº single tube ȤÀº single well ³»¿¡¼­ 3-stepÀ¸·Î ÁøÇàµÇ¸ç, 2½Ã°£ ³» ¿Ï·áµÈ´Ù. ½ÇÇè Áß°£¿¡ Á¤Á¦ °úÁ¤ÀÌ ÁøÇàµÇ°Å³ª, »ùÇÃÀ» ¿Å±â´Â °úÁ¤ÀÌ ºÒÇÊ¿äÇϱ⿡, ±×·Î ÀÎÇÑ »ùÇà ¼Õ½ÇÀ̳ª handling error¸¦ ¹æÁöÇÒ ¼ö ÀÖ´Ù
ThruPLEX¢ç Tag-Seq HV´Â ThruPLEX¢ç HV unique dual indexes (UDIs)¸¦ ÇÔ²² »ç¿ëÇÔÀ¸·Î½á 96-NGS-ready libraries¿¡ ´ëÇØ multiplex ºÐ¼®¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù. Á¤Á¦, Á¤·®ÀÌ ¿Ï·áµÈ library´Â ±âº»ÀûÀ¸·Î »ç¿ëµÇ´Â Illumina sequencing ½Ã¾à°ú ÇÁ·ÎÅäÄÝÀ» ÀÌ¿ëÇØ Illumina NGS ±â±â¿¡¼­ ¹Ù·Î ºÐ¼® °¡´ÉÇÏ´Ù. ÀÌ Á¦Ç°Àº de novo sequencing, whole genome resequencing, whole exome sequencing ȤÀº ÀÌ¿ÜÀÇ enrichment ±â¹ýÀÇ deep sequencingÀ» À§ÇÑ ³ôÀº coverage¿Í ÇÔ²² ÃÖ°íÀÇ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù. Cell-free plasma DNA ȤÀº FFPE À¯·¡ÀÇ ¼Õ»óµÈ DNA¿Í °°Àº ÀÛÀº Å©±âÀÇ DNA¿¡ °¡Àå ÀÌ»óÀûÀ¸·Î Àû¿ëÇÒ ¼ö ÀÖ´Ù.

ThruPLEX¢ç Tag-Seq HV PLUS Kit ³» Æ÷ÇԵǾî ÀÖ´Â ThruPLEX¢ç HV PLUS Enzymatic Fragmentation ModuleÀº enzymeÀ» ÀÌ¿ëÇÑ DNA fragmentation°ú repair °úÁ¤À» ÇÔ²² ¼öÇàÇÒ ¼ö ÀÖµµ·Ï °í¾ÈµÇ¾ú´Ù. µû¶ó¼­, ThruPLEX¢ç HV¸¦ ÀÌ¿ëÇÑ sequencing library Á¦ÀÛ »ùÇà Áغñ°úÁ¤¿¡¼­ º°µµÀÇ ±â±â³ª Ãß°¡ÀûÀÎ È¿¼Ò 󸮰úÁ¤ ¾øÀ̵µ ¿øÇÏ´Â ±æÀÌÀÇ DNA (300 bp, 450 bp)¸¦ ¾òÀ» ¼ö ÀÖ´Ù.


±×¸² 1. Single tube¿¡¼­ ThruPLEX¢ç Tag-Seq HV¸¦ ÀÌ¿ëÇÑ library Á¦ÀÛ °úÁ¤.
The ThruPLEX¢ç Tag-Seq HV workflow consists of three simple steps that take place in the same PCR tube or well and eliminates the need to purify or transfer the sample material.


±×¸² 2. ThruPLEX¢ç Tag-Seq HV ³» Æ÷ÇԵǾî ÀÖ´Â 144°³ Á¶ÇÕÀÌ °¡´ÉÇÑ unique molecular identifiers (UMIs)
The ThruPLEX¢ç Tag-Seq HV is designed to remove the ambiguity in variant calling by reducing the false positive calls coming from DNA polymerase and sequencing errors. The seven base UMIs are located at the beginning of the reads ensuring an easy demultiplexing of the samples to simplify the analysis


±×¸² 3. Human gDNA·ÎºÎÅÍ Á¦ÀÛµÈ library¿¡¼­ ³·Àº GC È®ÀÎ
Libraries were prepared from 5 ng and 50 ng of sheared human genomic DNA (Horizon Discovery Quantitative Multiplex Reference Standard, Cat. # HD701) using ThruPLEX¢ç Tag-Seq HV. Libraries were sequenced on an Illumina MiSeq¢ç instrument, and reads were aligned to the human genome (HG19) using bowtie2. The alignment metrics and GC bias were calculated using Picard tools.

  • All indexes have been functionally validated to work with Illumina sequencing systems (e.g., MiSeq¢ç, NovaSeq¢â, MiniSeq¢â, NextSeq¢ç, and HiSeq¢ç platforms) using two- or four-channel chemistry for base calling. They have not been validated with systems using one-channel chemistry.
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.

[ThruPLEX¢ç Tag-Seq HV]
- ThruPLEX Tag-Seq HV Core Components
- ThruPLEX HV UDI 1-24 or Set A
- Control Fragmented Human gDNA (5 ng / ¥ìl)

[ThruPLEX¢ç Tag-Seq HV PLUS Kit]
- ThruPLEX HV PLUS Enzymatic Fragmentation Module Set A
- ThruPLEX Tag-Seq HV Core Components
- ThruPLEX HV UDI 1-24 or Set A
- Control Fragmented Human gDNA (5 ng / ¥ìl)

Keyword : High volume,DNA-seq,FFPE,cfDNA,cell-free DNA,Tag-seq,UMI,Unique Molecular Index,DNA fragment,Enzymatic fragmentation,NGS,Next generation sequencing,NovaSeq,UDI, Unique dual index,Rubicon,·çºñÄÜ,Cancer research,¾Ï¿¬±¸,Cancer

High input DNA-seq
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FFPE, cell-free DNA-seq ºÐ¼® Á¦Ç°
ThruPLEX¢ç DNA-Seq HV
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UMI°¡ µµÀÔµÈ FFPE, cell-free DNA-seq ºÐ¼® Á¦..
ThruPLEX¢ç Tag-Seq HV