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°í¼Ó • °£Æí • °í°¨µµ¸¦ µ¿½Ã¿¡ ½ÇÇöÇÑ Àü±â¿µµ¿ ½Ã½ºÅÛ

FlashGel¢â System for RNA

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Lonza
57025
FlashGel¢â Dock
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ´ë
°¡°Ý¹®ÀÇ   FlashGel_System.pdf
Lonza
57040
FlashGel¢â Camera
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ´ë
°¡°Ý¹®ÀÇ   FlashGel_System.pdf
Lonza
57024
FlashGel¢â System for RNA Starter Pack
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 kit
(Package)
°¡°Ý¹®ÀÇ  
Lonza
50577
FlashGel¢â RNA Marker, 0.5 kb - 9 kb
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 §¡
°¡°Ý¹®ÀÇ   Flashgel markers.pdf
Lonza
51200
AccuGENE¢â Molecular Biology Water
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 L
°¡°Ý¹®ÀÇ  
Lonza
50462
FlashGel¢â Loading Dye (5X)
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ¡¿ 1 §¢
°¡°Ý¹®ÀÇ   Flashgel markers.pdf
Lonza
50571
Formaldehyde Sample Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ¡¿ 1 §¢
°¡°Ý¹®ÀÇ   Lonza_Formaldehyde_Buffer.pdf

FlashGel¢â System for RNA
FlashGel¢â System for RNA´Â º°µµÀÇ agarose Á¦ÀÛ°úÁ¤ ¾øÀÌ RNA »ùÇÃÀ» ºü¸£°Ô ½ºÅ©¸®´× ÇÒ ¼ö ÀÖ´Â Àü±â¿µµ¿ ½Ã½ºÅÛÀ¸·Î, º» ½Ã½ºÅÛÀ» ÀÌ¿ëÇÏ¸é °£ÆíÇÏ°Ô RNA ºÐ¸® ¹× purity È®ÀÎÀÌ °¡´ÉÇÏ´Ù.
FlashGel¢â RNA Cassettes¿¡´Â precast agarose gel, buffer, ¿°»ö½Ã¾àÀÌ Æ÷ÇԵǾî ÀÖ¾î, gel Á¦ÀÛ, buffer Ãß°¡ ¹× gel ¿°»ö ´Ü°è¸¦ »ý·«ÇÏ°í, cassettes¸¦ dock º»Ã¼¿¡ ÀåÂøÇØ »ùÇÃÀ» loading¸¸ Çϸé Àü±â ¿µµ¿À» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.

- 5ºÐ¸¸¿¡ Àü±â ¿µµ¿ÀÌ ¿Ï·á
FlashGel¢â SystemÀº º°µµÀÇ power supply·ÎºÎÅÍ 225 V±îÁö Àü¾ÐÀ» °É ¼ö ÀÖÀ¸¸ç, 0.5 - 9 kb ±îÁöÀÇ RNA ´ÜÆí ºÐ¸®°¡ 3-8ºÐÀÌ¸é ¿Ï·áµÈ´Ù (single-tier: 225 V 4-8 ºÐ, double-tier: 225 V 3-5 ºÐ).
*¹êµå¸¦ ¼±¸íÈ÷ °üÂûÇÏ·Á¸é, Àü±â¿µµ¿ ÈÄ 10~20ºÐ incubation½Ã°£À» ÁÖ´Â °ÍÀÌ ÁÁ´Ù.

[FlashGel¢â System for RNA¸¦ ÀÌ¿ëÇÑ RNA Àü±â¿µµ¿ ¿¹]
Lane 1: Samples of RNA Marker (Lonza), 50 ng
Lane 2: Samples of RNA Marker (Lonza), 250 ng
Lane 3: E. coli total RNA, 50 ng
Lane 4: E. coli total RNA, 250 ng per 5 ¥ìl load.
Separation of Total RNA on the FlashGel¢â System for RNA
Samples prepared with Formaldehyde Sample Buffer (Lonza) and denatured 5 minutes at 65¡ÆC. FlashGel¢â RNA Cassette run for 8 minutes, followed by a 20 minutes hold prior to imaging.

[FlashGel¢â System for RNA¸¦ ÀÌ¿ëÇÑ RNA quality È®ÀÎ ¿¹]
Lane 1: RNA marker (Lonza)
Lane 2: 250 ng E. coli Total RNA

Lanes 3 - 7: E. coli Total RNA incubated with increasing levels of RNase A.
Sample degradation is visible at low levels on the FlashGel¢â System for RNA.
FlashGel¢â RNA Cassette run for 8 minutes at 225 V, followed by 20 minutes hold prior to imaging.
Intact, denatured RNA shows sharp, clear bands on the FlashGel¢â System. Partially degraded RNA has a smeared appearance, and completely degraded RNA appears as a low molecular weight smear.

- °í°¨µµ °ËÃâ
FlashGel¢â System for RNA ¿¡¼­´Â ÀϹÝÀûÀÎ EtBr ¿°»ö gel°ú ºñ±³ÇÏ¿© 5-20¹èÀÇ ³ôÀº °¨µµ·Î ¹êµå¸¦ È®ÀÎÇÒ ¼ö ÀÖ°í, ¾à 10 ng RNA/bandÀÇ °ËÃâÀÌ °¡´ÉÇϱ⠶§¹®¿¡, ¾çÀÌ ÀûÀº »ùÇÃÀÇ °ËÃâ¿¡µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù.

- °£ÆíÇÑ Àü¿ë cassettes »ç¿ë (RNase free)
FlashGel¢â RNA Cassettes¿¡´Â precast agarose gel, buffer, gel ¿°»ö ½Ã¾àÀÌ Æ÷ÇԵǾî ÀÖ´Ù. GelÀ» ÁغñÇÏ´Â ¹ø°Å·Î¿ò ¾øÀÌ ¸î ºÐ ¾È¿¡ Àü±â¿µµ¿ °á°ú¸¦ ¾òÀ» ¼ö Àֱ⠶§¹®¿¡ Á¤Á¦ÇÑ total RNAÀÇ ½Å¼ÓÇÑ È®Àο¡ ÃÖÀûÀÌ´Ù.
½Ã½ºÅÛ ±¸¼º

Code

Á¦Ç°¸í

Dock

Camera

Cassettes

Accessories
(marker, loading dye)

57067

FlashGel¢â System

+

+

+

+

57026

FlashGel¢â DNA Starter Kit

+

-

+

+

57063

FlashGel¢â DNA Kit

-

-

+

+

57025

FlashGel¢â Dock

+

-

-

-

57040

FlashGel¢â Camera

-

+

-

-

57065

FlashGel¢â Camera Pack

+

+

-

-

57024

FlashGel¢â RNA Starter Kit

-

-

+

+

Á¦Ç°º° ¼¼ºÎ±¸¼º

Code

Á¦Ç°¸í

±¸¼ºÇ°

57024

FlashGel¢â System for RNA Starter Pack

FlashGel¢â RNA Cassettes 1.2% (12+1 well) (#57027)
Formaldehyde Sample Buffer (with bromophenol blue and xylene cyanol)
RNA Marker 0.5 bp - 9 kb
Molecular Biology Grade Water (#51200)

57065

FlashGel¢â Camera Pack

FlashGel¢â Dock (#57025)
FlashGel¢â Camera (#57040)

°¢ ±¸¼ºÇ° »ç¾ç
1. FlashGel¢â Dock*
Dock size£º134 mm¡¿120 mm¡¿54 mm

*º°µµÀÇ power supply ÀåÄ¡ ÇÊ¿ä (225 VÀÇ °ø±Þ ´É·Â)

2. FlashGel¢â RNA Cassettes
- RNA Cassettes »ç¾ç

Cassettes size

115 mm (L) ¡¿ 107 mm (W) ¡¿ 17 mm (H)

Gel size

70 mm (L) ¡¿ 84 mm (W) ¡¿ 2 mm (H)

Agarose concentration & Separation range

1.2 %: 0.5 bp-9 kb

»ùÇà ·Îµù¾ç

5 §¡/well ÀÌÇÏ

*Cassettes Æó±â ¹æ¹ý: EtBr ó¸® ¹æ¹ý°ú µ¿ÀÏÇÏ°Ô Æó±â, Cassettes ¿Í °ÖÀº ºÐ¸® ºÒ°¡

- Cassettes Á¾·ù ¹× ±Ô°Ý

Code

Àû¿ë

Agarose ³óµµ

Well ¼ö

Tiers

57027

RNA

1.2%

12+1

Single-tier

57028

RNA

1.2%

16+1

double-tier

*ÀϹÝÀûÀÎ DNA Àü±â¿µµ¿¿ë cassettes´Â ¸µÅ© ÆäÀÌÁö Âü°í

3. ±× ¿Ü

Code

Á¦Ç°¸í

³»¿ë

50577

FlashGel¢â RNA Marker, 0.5 kb - 9 kb

500, 1000, 1500, 3000, 5000, 9000 bp
: Formaldehyde Sample Buffer (Code 50571)°ú È¥ÇÕÇÏ¿© »ç¿ë(65¡É 5ºÐ).

50571

Formaldehyde Sample Buffer

MOPS buffer¿¡ formaldehyde, formamide, tracking dye (Bromophenol Blue, Xylene Cyanol)Æ÷ÇÔ
: denature RNA »ùÇÃÀ» Á¶Á¦ ½Ã RNA ¿ë¾×°ú °°Àº ¾çÀ» È¥ÇÕÇØ »ç¿ë.

*±× ¿Ü FlashGel¢â DNA Marker´Â ¸µÅ© ÆäÀÌÁö Âü°í

4. FlashGel¢â Camera
- USB ÄÉÀ̺íÀ» ÅëÇØ PC³ª ³ëÆ®ºÏ¿¡ Á÷Á¢ ¿¬°áÇؼ­ »ç¿ë °¡´É
º¸Á¸
FlashGel¢â RNA Cassettes: ½Ç¿Â (18-26¡É) Á¦Á¶ ÈÄ 3°³¿ù
°ü·ÃÁ¦Ç°
- gDNA Eraser ColumnÀ¸·Î gDNA ¿À¿° ¾øÀÌ ´Ù¾çÇÑ »ùÇÃÀÇ RNA Á¤Á¦: TaKaRa MiniBEST Universal RNA Extraction Kit
- ºü¸£°í Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ RT premix: PrimeScript¢â IV 1st strand cDNA Synthesis Mix
- ½ÇÇ躰 PCR È¿¼Ò ¼±ÅÃÇϱâ
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP
- ½Å¼Ó°£Æí DNA Àü±â¿µµ¿ ½Ã½ºÅÛ: FlashGel¢â System for DNA

Keyword :

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FlashGel¢â System for DNA
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FlashGel¢â System for RNA
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FlashGel¢â System for DNA Recovery