¿À´Ã ÇÏ·ç ±×¸¸º¸±â
´Ý±â
·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø
¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Á¦Ç°Á¤º¸
°øÁö
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
Learning Center
ȸ»çÁ¤º¸
ÀüÁ¦Ç°º¸±â
Á¦Ç°¼±Åð¡À̵å
Custom Service
À̺¥Æ®/ÇÁ·Î¸ð¼Ç
´ÙÄ«¶ó¼Ò½Ä
Takara Bio°ø½Ã
½ÅÁ¦Ç°¾È³»
¶óÀ̼±½º¾È³»
±â¼úÁö¿ø¹®ÀÇ
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LMO¾È³»
ºê·Î¼Å
On-line tool
eNews
樨毢
CoA & MSDS
°³¿ä & ¸®½ºÆ®
¼Ò°³
µµÀԺ귣µå Á¤º¸
´ë¸®Á¡Á¤º¸
ã¾Æ¿À½Ã´Â±æ
Home
>
ÀüÁ¦Ç°º¸±â
>
Stem Cell
>
iPS cell Á¦ÀÛ ¹× °ËÃâ
> Retrovirus Titer Set (for Real Time PCR)
Virus »óûÀ¸·Î ºÎÅÍ Á÷Á¢ 4 ½Ã°£¿¡ RNA Titer¸¦ ÃøÁ¤
Retrovirus Titer Set (for Real Time PCR)
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Takara
6166
Retrovirus Titer Set (for Real Time PCR)
100 ¹ÝÀÀºÐ
348,800¿ø
436,000¿ø
09.02 ~ 10.31
Product index
Overview
Áٱ⼼Æ÷ ¼±Åð¡À̵å
Áúº´ ¸ðµ¨ °³¹ßÀ» À§ÇÑ ..
Custom service (Áٱ⼼..
[Cancer Research] Gene..
´Ù¾çÇÑ Áٱ⼼Æ÷ Á¦Ç°ÀÇ..
Custom Product (¹èÁö..
hiPSC/hESC
½Å¾à°³¹ßÀ» À§ÇÑ iPSC ..
DEF-CS¢â Overview: ÇÑ ..
[Àû¿ë] iPS cell reprog..
[»ç¿ë Èıâ] Â÷¼¼´ë ÁÙ..
Human iPS Cell Line Ki..
DEF-CS 500 Culture Sys..
Xeno-free & GMP DEF-CS..
Xeno-Free 3D Spheroid ..
Áٱ⼼Æ÷ À¯ÀüÀÚÆíÁý
hiPSCÀÇ À¯ÀüÀÚ ÆíÁýÀ» ..
iPSC Single-Cell Cloni..
MSC (Mesenchymal Stem Cell..
Human MSC xeno-free cu..
Mesenchymal progenitor..
Hepatocyte
[Àû¿ë] hiPS-HEP cells ..
[Àû¿ë] hiPS-HEP cells ..
Primary hepatocyte med..
iPS Cell to Hepatocyte..
Beta cell
Definitive Endoderm ce..
hiPSC to DE(definitive..
Neural Stem cell (NSC)
½Å°æÁٱ⼼Æ÷ ¿¬±¸ÀÇ To..
[Àû¿ë] NDiff 227À» ÀÌ..
RHB-A/RHB Basal
NDiff N2
NDiff277
Endothelial Cell
Endothelial Cells from..
iPS Cell to Endothelia..
Mouse stem cell °ü·Ã Á¦Ç°
[Àû¿ë] NDiff 227À» ÀÌ..
Cellartis¢ç 3i mESC/iP..
Cellartis¢ç 2i mESC/iP..
RHB-A/RHB Basal
NDiff227
NDiff N2
Osteoblast-Inducer Rea..
iPS cell Á¦ÀÛ ¹× °ËÃâ
Stem antibodies
Human ES / iPS ¼¼Æ÷°ü..
Human Mitochondrial DN..
Pluripotency Check PCR..
PrimerArray¢ç Embryoni..
Provirus Copy Number D..
Retrovirus Packaging K..
Retrovirus Titer Set (..
Coating matrix
Easy iMatrix-511 / Eas..
iMatrix-511 / iMatirx-..
³»¿ë (100 ¹ÝÀÀ ºÐ ; 25§¡ ¹ÝÀÀ°è
*1
)
Forward Titer Primer FRT-1 (10 pmol/§¡)
50 §¡
Reverse Titer primer RRT-1 (10 pmol/§¡)
50 §¡
RNA Control Template (5 ¡¿ 10
8
copies/§¡)
10 §¡
EASY Dilution (Real Time PCR ¿ë)
1 §¢ ¡¿ 2
DNase I (RNase Free) (5 U/§¡)
40 §¡
10 ¡¿ DNase I Buffer
50 §¡
RNase Inhibitor (porcine liver) (40 U/§¡)
10 §¡
*1
Real Time RT- PCR 100ȸ ±×¸®°í DNase I ó¸® 20ȸÀÓ.
º¸Á¸ -20¡É
RNA Control Template´Â ¹Ýº¹ÀûÀÎ µ¿°á À¶ÇØ¿¡ ÀÇÇÑ ºÐÇظ¦ ¸·±â À§ÇÏ¿© ¼Ò·® ºÐÁÖ ÈÄ Deep freezer (-70¡-80¡É)¿¡¼ ÀúÀåÇÏ´Â °ÍÀ» ÃßõÇÕ´Ï´Ù.
Á¦Ç°¼³¸í
º» Á¦Ç°Àº Real Time RT-PCRÀ» ÀÌ¿ëÇÏ¿© MLV (murine leukemia virus) À¯·¡ retrovirus vectorÀÇ RNA genome ·®À» ÃøÁ¤ÇÏ¿© ¿ª°¡(RNA titer)À» »êÃâÇÏ´Â Á¦Ç°ÀÌ´Ù.
º» Á¦Ç°À» One Step TB Green¢â PrimeScript RT-PCR Kit (Perfect Real Time) (Code RR066A/RR086A)°ú ÇÔ²² »ç¿ëÇÏ¿© ¹ÙÀÌ·¯½º »óµî¾×¿¡¼ Á÷Á¢ RNA titer¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ°í, ¾à 4½Ã°£ ¾È¿¡ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù. ¶ÇÇÑ, MLVÀÇ packaging signalºÎºÐÀ» ÁõÆø ´ë»óÀ¸·Î ÇÏ°í ÀÖ¾î °ÅÀÇ ¸ðµç MLV À¯·¡ RetrovirusÀÇ ¿ª°¡(RNA titer)¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù.
RetrovirusÀÇ °¨¿° ¿ª°¡¸¦ ÃøÁ¤ÇÒ °æ¿ì ÀϹÝÀûÀ¸·Î Ç¥Àû ¼¼Æ÷¿¡ Retrovirus¸¦ °¨¿°½ÃÅ°°í Çü±¤À̳ª ¾àÁ¦³»¼º µîÀ» ÀÌ¿ëÇÏ¿© °¨¿°È¿À²À» ÃøÁ¤ÇØ ¿ª°¡ (»ý¹°ÇÐÀû titer)À» »êÃâÇÏ´Â ¹æ¹ýÀÌ ÀÌ¿ëµÈ´Ù. ÀÌ ¹æ¹ýÀº ¼öÀÏÀÌ ¼Ò¿äµÇ¸ç, ¸¶Ä¿À¯ÀüÀÚ³ª ¾àÁ¦³»¼ºÀ¯ÀüÀÚ°¡ Æ÷ÇÔµÇÁö ¾ÊÀº Retrovirus vector¿¡´Â Àû¿ëÇÒ ¼ö ¾ø´Ù. ¶ÇÇÑ »ý¹°ÇÐÀû titer¸¦ ±âÁØÀ¸·Î MOI (multiplicity of infection)¸¦ ÃøÁ¤ÇØ ¼¼Æ÷¿¡ °¨¿°½Ãų °æ¿ì, ¿ª°¡ ÃøÁ¤¿¡ ¸çÄ¥ÀÌ ¼Ò¿äµÇ¹Ç·Î ÀÏ´Ü ¹ÙÀÌ·¯½º »óµî¾×À» µ¿°á º¸Á¸ÇÒ ÇÊ¿ä°¡ ÀÖ°í, µ¿°á À¶ÇØ¿¡ ÀÇÇØ ¹ÙÀÌ·¯½º ¿ª°¡°¡ ÀúÇ쵃 °¡´É¼ºÀÌ ÀÖÀ¸¹Ç·Î Ãʱâ ÃøÁ¤°ª°ú´Â ´Ù¸¥ MOIÀÇ ¹ÙÀÌ·¯½º°¡ ½ÇÇè¿¡ ÀÌ¿ëµÉ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº RetrovirusÀÇ RNA genome·®À» ÃøÁ¤Çϱ⠶§¹®¿¡ ¸¶Ä¿À¯ÀüÀÚ³ª ¾àÁ¦³»¼ºÀ¯ÀüÀÚ¿Í´Â °ü°è¾øÀÌ ¿ª°¡¸¦ ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ¾à 4½Ã°£ ¸¸¿¡ ÃøÁ¤ °á°ú¸¦ ¾òÀ» ¼ö ÀÖÀ¸¹Ç·Î ¹ÙÀÌ·¯½º »óµî¾×À» µ¿°áÇÏÁö ¾Ê°í ¿øÇÏ´Â MOI·Î °¨¿°½Ãų ¼ö ÀÖ´Ù. ÇÔ²² »ç¿ëÇÏ´Â One Step TB Green¢â PrimeScript RTPCR Kit (Perfect Real Time) Àº TB Green¢â °ËÃâ¿¡ ÀÇÇÑ 1 step Real Time RT-PCR Å°Æ®ÀÌ´Ù. ½ÅÀ强ÀÌ ¶Ù¾î³ª ´Ü½Ã°£¿¡ È¿À²ÀûÀ¸·Î cDNA ÇÕ¼ºÀÌ °¡´ÉÇÑ ¿ªÀü»ç È¿¼Ò PrimeScript ¿Í ´ÙÄ«¶óÀÇ ´ëÇ¥ÀûÀÎ PCR È¿¼ÒÀÎ
TaKaRa Ex Taq
HS¸¦ ÀÌ¿ëÇÏ¿© 1 step RT-PCR¿¡ ÃÖÀûÈÇÏ°í ÀÖÀ¸¸ç ¾ÈÁ¤ÀûÀ¸·Î 1 step real time RT-PCRÀ» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù.
ÀÌ¿Ü¿¡ ÇÊ¿äÇÑ ½Ã¾à
One Step TB Green¢â PrimeScript RT-PCR Kit (Perfect Real Time) (TaKaRa Code RR066A)
¶Ç´Â One Step TB Green¢â PrimeScript RT-PCR Kit II (Perfect Real Time) (TaKaRa Code RR086A)
Keyword :
iPS cell Á¦ÀÛ ¹× °ËÃâ
Stem antibodies
Human ES / iPS ¼¼Æ÷°ü·Ã Ç×ü
¹ÌÅäÄܵ帮¾Æ DNA¸¦ ÁöÇ¥·Î ´Ù´É¼º¼¼Æ÷¿¡¼ ÁÙ..
Human Mitochondrial DNA (mtDNA) Monitoring Primer Set
Pluripotency Check PCR Kit
ES¼¼Æ÷/iPS¼¼Æ÷ÀÇ ´ÙºÐÈ°ü·Ã À¯ÀüÀÚÀÇ ¹ßÇö ÇØ..
PrimerArray¢ç Embryonic Stem Cells
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus copy ¼ö¸¦ ..
Provirus Copy Number Detection Primer Set, Human (for Real Time PCR)
ÀÏ°ú¼ºÀ¸·Î ³ôÀº titerÀÇ Retrovirus ÀÔÀÚ¸¦ »ý..
Retrovirus Packaging Kit
Virus »óûÀ¸·Î ºÎÅÍ Á÷Á¢ 4 ½Ã°£¿¡ RNA Titer..
Retrovirus Titer Set (for Real Time PCR)