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ÀüÁ¦Ç°º¸±â
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Inducible System ¡¤ Yeast hybrid
>
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
> Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
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°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
631164
Tet-On 3G Inducible Expression System (with ZsGreen1)
Each
2,062,000¿ø
,
,
,
,
Clontech
631165
Tet-On 3G Inducible Expression System (with mCherry)
Each
2,062,000¿ø
,
,
,
,
,
,
Clontech
631167
Tet-On 3G Inducible Expression System (EF1¥á Version)
Each
2,062,000¿ø
,
,
,
Clontech
631168
Tet-On 3G Inducible Expression System
Each
2,062,000¿ø
,
,
,
,
Clontech
631187
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System
each
(Package)
3,551,000¿ø
,
,
Clontech
631188
Retro-X Tet-On 3G Inducible Expression System
Each
(Package)
3,168,000¿ø
,
,
,
,
,
Clontech
631348
Tet-On 3G Inducible Expression System (EF1alpha, ZsGreen1)
each
2,432,000¿ø
,
,
,
Clontech
631337
Tet-On 3G Bidirectional Inducible Expression System
Each
2,292,000¿ø
,
,
,
Clontech
631338
Tet-On 3G Bidirectional Inducible Expression System (with mCherry)
Each
2,432,000¿ø
,
,
,
Clontech
631339
Tet-On 3G Bidirectional Inducible Expression System (with ZsGreen1)
Each
2,432,000¿ø
,
,
,
Clontech
631340
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha Version)
Each
2,292,000¿ø
,
,
,
Clontech
631341
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, mCherry)
Each
2,432,000¿ø
,
,
,
Clontech
631342
Tet-On 3G Bidirectional Inducible Expression System (EF1alpha, ZsGreen1)
Each
2,432,000¿ø
,
,
,
Clontech
631346
Tet-On 3G Inducible Expression System (EF1alpha, Bicistronic)
Each
2,292,000¿ø
,
,
,
Clontech
631347
Tet-On 3G Inducible Expression System (EF1alpha, mCherry)
Each
2,432,000¿ø
,
,
,
Clontech
631363
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha Version)
Each
(Package)
3,551,000¿ø
,
,
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
DoxycyclineÀÇ À¯/¹«¿¡ µû¶ó À¯ÀüÀÚ ¹ßÇö (transcription)ÀÌ Á¶ÀýµÇ´Â inducible system
¸Å¿ì ³·Àº Background¹ßÇö
Mammalian cell¿¡¼ ƯÀ̼ºÀÌ ¸Å¿ì ³ôÀº À¯ÀüÀÚ ¹ßÇö À¯µµ °¡´É
Á¦Ç°¼³¸í
Tet-On
¢ç
Àº doxycyclineÀÇ À¯/¹«¿¡ µû¶ó À¯ÀüÀÚ ¹ßÇö (transcription)ÀÌ Á¶ÀýµÇ´Â inducible systemÀÌ´Ù. ÀÌÁß Tet-On
¢ç
3G tetracycline-inducible gene expression systemÀº 3¼¼´ëÀÇ Tet-On
¢ç
systemÀ¸·Î ´Ù¾çÇÑ ºÐ¾ß¿¡¼ »ç¿ëµÇ°í ÀÖ´Ù. ÀÌ´Â 1¼¼´ë¿Í 2¼¼´ëÀÎ Tet-On
¢ç
°ú Tet-On
¢ç
Advanced system ´ëºñ background ¹ßÇöÀ» ³·Ãß°í tetracycline À¯»çüÀÎ doxycycline¿¡ ´ëÇÑ ¹Î°¨µµ¸¦ Çâ»ó½ÃŲ ±â¼úÀÌ´Ù.
3¼¼´ë Tet-on À¯µµ ¹ßÇö ½Ã½ºÅÛÀº CMV promoter¸¦ »ç¿ëÇØ Tet-On
¢ç
3G transactivator¸¦ ¹ßÇöÇÏ´Â ±âº» ½Ã½ºÅÛ Tet-On
¢ç
3G Inducible Expression SystemÀ» Æ÷ÇÔÇÑ ´Ù¾çÇÑ ÇüÅ·ΠÆǸŵǰí ÀÖ´Ù.
Tet-On
¢ç
3G Inducible Expression System (EF1alpha Version) (Code 631167 ¿Ü)
: EF1¥á promoter¸¦ »ç¿ëÇÏ¿© CMV promoter¿¡¼´Â ½Ã°£ÀÌ Áö³¯¼ö·Ï ¹ßÇöÀÌ °¨¼ÒÇÏ´Â ¼¼Æ÷ (e.g. hematopoietic cells, stem cells)¿¡¼µµ Tet-On
¢ç
3G transactivator¸¦ ÀÏÁ¤ÇÏ°Ô Àå±â°£ ¹ßÇöÇÒ ¼ö ÀÖ´Ù.
Tet-On
¢ç
3G Bidirectional Inducible Expression System (Code 631340 ¿Ü)
: Bidirectional promoter¸¦ »ç¿ëÇÏ¿© µÎ°¡Áö À¯ÀüÀÚ ¹ßÇöÀ» µ¿½Ã¿¡ À¯µµÇÒ ¼ö ÀÖ´Ù. À̶§ µÎ À¯ÀüÀÚ ¸ðµÎ µ¿µîÇÑ ¼öÁØÀ¸·Î ¹ßÇöµÇ¸ç À̸¦ ´Ù¾çÇÑ ÀÀ¿ë ºÐ¾ß¿¡¼ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
- °°Àº ¼¼Æ÷¿¡¼ µÎ Á¾·ùÀÇ ¸ñÀû À¯ÀüÀÚ¸¦ µ¿½Ã ¹ßÇö
- Á÷/°£Á¢ÀûÀÎ ÃøÁ¤ÀÌ ¾î·Á¿î ¸ñÀû À¯ÀüÀÚÀÇ ¹ßÇöÀ» ¸®Æ÷ÅÍ À¯ÀüÀÚÀÇ µ¿½Ã ¹ßÇöÀ¸·Î ¸ð´ÏÅ͸µ
- ¸®Æ÷ÅÍ À¯ÀüÀÚÀÇ ¹ßÇöÀ» ¸ð´ÏÅ͸µÇÏ¿© À¯µµ ¹ßÇöÀÌ ³ô°í background°¡ ³·Àº clone ¼±º°
Tet-On
¢ç
3G Inducible Expression System (with mCherry / with ZsGreen1 ¿Ü) (Code 631342 ¿Ü)
: IRES (internal ribosome entry site) ¼¿À» »ç¿ëÇØ ¸ñÀû À¯ÀüÀÚ¿Í Àû»ö (with mCherry) ȤÀº ³ì»ö (with ZsGreen1) Çü±¤ ´Ü¹éÁúÀ» °°ÀÌ ¹ßÇö½Ãų ¼ö ÀÖ´Ù. Çü±¤ ´Ü¹éÁú°ú ¸ñÀû À¯ÀüÀÚ´Â µ¿ÀÏÇÑ RNA Àü»çü¿¡¼ º°µµ·Î µÎ ´Ü¹éÁú·Î À¯µµ ¹ßÇöµÇ¸ç ÀÌ Çü±¤ ´Ü¹éÁúÀ» »ç¿ëÇØ doxycycline ÷°¡ ½Ã ¸ñÀû À¯ÀüÀÚÀÇ À¯µµ ¹ßÇö È¿À²ÀÌ ³ôÀº cloneÀ» ¼±º°Çϴµ¥ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
Tet-One
¢ç
system (Code 634301 ¿Ü)
: ÀϹÝÀûÀ¸·Î »ç¿ëÇÏ´Â Tet-On
¢ç
3G system˼ Tet-On
¢ç
3G transactivator¿Í tetracycline¿¡ ¹ÝÀÀÇÏ´Â À¯µµ ¹ßÇö promoter (PTRE3G)¸¦ °¢°¢ °¡Áö°í ÀÖ´Â 2 vector systemÀ̳ª, º» Á¦Ç°Àº 1 vector systemÀ¸·Î ½ÇÇèÀÇ ÆíÀǼºÀ» Áõ°¡½ÃÄ×´Ù.
P
TRE3GV promoter¸¦ °¡Áø lentivirus¶Ç´Â retrovirus¸¦ »ý»êÇÏ´Â
Lenti-X¢â/Retro-X¢â Tet-On
¢ç
3G Inducible Expression System
À» »ç¿ëÇϸé virus »ý»ê¿¡ ÇÊ¿äÇÑ viral packaging systemÀ» Çѹø¿¡ ÁغñÇÒ ¼ö ÀÖ´Ù.
³»¿ë
- °¢ Á¦Ç°ÀÇ »ç¿ëÀÚ ¸Å´º¾óÀ» Âü°íÇϼ¼¿ä.
ÁÖÀÇ) ÀÌ Á¦Ç°¿¡ Æ÷ÇÔµÈ pTRE vector´Â plasmid Á¦ÀÛ ½Ã ÀϹÝÀûÀÎ plasmidº¸´Ù ¼öÀ²ÀÌ ³·´Ù. µû¶ó¼ ¼öÀ²À» ³ôÀ̱â À§ÇØ Æò¼Òº¸´Ù ¹è¾ç¾× ¾çÀ» ´Ã·Á¼ »ç¿ëÇÏ´Â °ÍÀ» ÃßõÇÑ´Ù.
¶ÇÇÑ, ½ÃÁßÀÇ plasmid purification kit¸¦ »ç¿ëÇÏ´Â °æ¿ì low copy plasmid¿ë ÇÁ·ÎÅäÄÝÀ» »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÑ´Ù.
Ư¡
- Bakcground °¨¼Ò
: promoter ¼¿À» ÃÖÀûÈÇÏ¿© ±âÁ¸ÀÇ
P
TIGHT ´ëºñ background ¹ßÇöÀ» 5~20¹è·Î °¨¼Ò½ÃÄ×´Ù. (±×¸² 1.)
- Dox¿¡ ´ëÇÑ ¹Î°¨µµ Áõ°¡
: Tet-On
¢ç
3G regulator vectorÀÇ ¼¿À» ÃÖÀûÈÇÏ¿© doxycycline¿¡ ´ëÇÑ ¹Î°¨µµ°¡ ´ëÆø Áõ°¡µÇ¾î º¸´Ù ³·Àº ³óµµÀÇ Dox (5~10 ng/§¢)¿¡¼µµ ¹ßÇöÀ» À¯µµÇÒ ¼ö ÀÖ´Ù. ´Ü 10 ng/§¢¸¦ ÇÏ·í¹ã ó¸®ÇÏ´Â °ÍÀ¸·Î ¸Å¿ì ³ôÀº ¹ßÇöÀ» À¯µµÇÒ ¼ö ÀÖ´Â °í°¨µµÀÇ ½Ã½ºÅÛÀÌ´Ù.
- °ÇÑ ¹ßÇö À¯µµ ºñÀ²
: Tet-On
¢ç
3G´Â ±âÁ¸ÀÇ Tet-On ¹× Tet-Off system°ú °°ÀÌ ³ôÀº ÃÖ´ë ¹ßÇö ¼öÁØÀ» À¯ÁöÇϸ鼵µ background¸¦ Å©°Ô ÁÙ¿´±â ¶§¹®¿¡ ¹ßÇö À¯µµ ½Ã ¸Å¿ì ³ôÀº ¹ßÇö À¯µµÀ²À» º¸Àδ٠(±×¸² 2). MCF7 ¼¼Æ÷¸¦ ÀÌ¿ëÇÑ ½ÇÇè¿¡¼´Â ÃÖ´ë 27,000¹èÀÇ À¯µµ ºñÀ²ÀÌ È®ÀεǾú´Ù.
Tet System Approved FBS
Tet-On
¢ç
3G system˼
Tet System Approved FBS (Code 631107 ¿Ü)
¿Í ÇÔ²² »ç¿ëÇÏ´Â °ÍÀÌ Áß¿äÇÏ´Ù. Tet-On
¢ç
3G system doxycycline¿¡ ´ëÇÑ °¨µµ°¡ ¸Å¿ì Çâ»óµÇ¾ú±â ¶§¹®¿¡ tetracyclineÀÌ Æ÷ÇÔµÇÁö ¾ÊÀº FBS¸¦ »ç¿ëÇØ¾ß ÇÑ´Ù. Tet System Approved FBS (Code 631107 ¿Ü)´Â Tet-On
¢ç
3G systemÀÇ background¹ßÇö¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾Ê´Â Á¡ÀÌ °ËÁõµÇ¾ú±â ¶§¹®¿¡ ÇØ´ç Á¦Ç°À» »ç¿ëÇÏ´Â °ÍÀ» ÃßõÇÑ´Ù. °¢ Tet-On
¢ç
3G system¿¡´Â Tet System Approved FBS (Code 631107 ¿Ü) 50 §¢ÀÌ Æ÷ÇԵǾî ÀÖ´Ù.
±×¸² 1.
P
TRE3G ÇÁ·Î¸ðÅÍÀÇ ³·Àº background¹ßÇö
Tet-On
¢ç
3G system°ú Tet-On
¢ç
Advanced systemÀ» »ç¿ëÇÏ¿© HEK293 ¼¼Æ÷¿¡ °¢°¢ regulatory vector¿Í response vector (luciferase)¸¦ transfection ÇÏ¿´´Ù. ÀÌÈÄ ¼¼Æ÷¸¦ Dox +/- (0, 50, 500 ng/§¢) »óÅ·Π24½Ã°£ ¹è¾ç ÈÄ luciferaseÀÇ ¹ßÇöÀ» ÃøÁ¤ÇÏ¿´´Ù. ±× °á°ú, Dox°¡ ¾ø´Â »óÅ¿¡¼´Â Tet-On
¢ç
3G system¿¡¼´Â background ¹ßÇöÀÌ ¸Å¿ì ³·¾Ò°í (È®´ëµÈ ºÎºÐ), Dox°¡ ó¸®µÈ Á¶°Ç¿¡¼´Â µÑ´Ù ¸Å¿ì °ÇÑ ¹ßÇöÀ» º¸ÀÌ´Â °ÍÀ» È®ÀÎÇÒ ¼ö ÀÖ´Ù.
±×¸²2. ¹ßÇö À¯µµÀ² (induction rate)ÀÌ Çâ»óµÈ Tet-On
¢ç
3G ½Ã½ºÅÛ
Tet-On
¢ç
, Tet-On
¢ç
Advanced, Tet-On
¢ç
3G ¼¼°¡Áö ½Ã½ºÅÛÀ» »ç¿ëÇØ HEK 293 cell°ú HeLa cell¿¡ regulatory vector¿Í response vector (luciferase)¸¦ transfection ÈÄ 1 §¶/§¢ doxycyclineÀ¸·Î luciferase À¯ÀüÀÚ¸¦ ¹ßÇö½ÃÄ×´Ù. ÀÌÈÄ 18½Ã°£ ÀÌÈÄ 3¹Ýº¹À¸·Î luciferase È°¼ºÀ» ÃøÁ¤ÇÑ °á°ú Tet-On
¢ç
3G systemÀÌ °¡Àå ³ôÀº ¹ßÇö À¯µµÀ²À» º¸ÀÌ´Â °ÍÀ» È®ÀÎÇÒ ¼ö ÀÖ¾ú´Ù.
±×¸²3. ¹Î°¨µµ°¡ Çâ»óµÈ Tet-On
¢ç
3G ½Ã½ºÅÛ
GenomeÀÇ °°Àº À§Ä¡¿¡¼Tet-On
¢ç
3G ¿Í Tet-On
¢ç
AdvancedÀÇ µÎ °¡Áö ÇÁ·Î¸ðÅÍ¿¡ ÀÇÇØ ¾ÈÁ¤ÀûÀ¸·Î luciferase À¯ÀüÀÚ¸¦ ¹ßÇöÇÏ´Â HLF33 cell-line¿¡¼ Dox ³óµµ¸¦ ¹Ù²ã°¡¸ç luciferaseÀÇ ¹ßÇö·®À» ÃøÁ¤ÇÏ¿´´Ù. ±× °á°ú 5~10 ng/§¢¿¡¼´Â Tet-On
¢ç
3GÀÇ cell-line¿¡¼ 100~150¹è ³ô¾Ò°í, 50 ng/§¢¿¡¼´Â ¹ßÇö·®ÀÌ 4.6¹è ³ô¾ÆÁ³´Ù.
(University of Erlangen, W. Hillen
et. al
)
±×¸²4. 10 ng/§¢ÀÇ Dox¿¡¼µµ °í°¨µµ·Î ÀÛµ¿ÇÏ´Â Tet-On
¢ç
3G
±×¸²5. ¸Å¿ì ³ôÀº S/N ratioÀÇ Tet-On
¢ç
3G¸¦ »ç¿ëÇÑ ¼¼Æ÷ÁÖ
¼¼ Á¾·ùÀÇ Tet-On
¢ç
3G cell-line¿¡ response vector (luciferase)¸¦ transfection Çß´Ù. ÀÌÈÄ Dox +/- »óÅ¿¡¼ 24½Ã°£ ¹è¾ç ÈÄ luciferase È°¼ºÀ» ÃøÁ¤ÇÑ °á°ú, ¸ðµÎ ¼öõ¹èÀÇ S/N ratio¸¦ º¸¿´´Ù.
¿ëµµ
- Mammalian cell¿¡¼ À¯ÀüÀÚ ¹ßÇö À¯µµ
- µ¶¼º À¯ÀüÀÚ ¹ßÇö Á¦¾î
-
in vivo
¿¡¼ÀÇ ¸ñÀû À¯ÀüÀÚ ¹ßÇö À¯µµ
°ü·ÃÁ¦Ç°
- Any vector, any insert, any site·Î DNA assembly,
In-Fusion
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Snap Assembly Master Mix (Code 638947)
- In-Fusion
¢ç
¿¡¼ ÃÖ°íÀÇ cloning È¿À²À» À§ÇÑ Àü¿ë
E.coli
comp.cell,
Stellar¢â Competent Cells (Code 636763)
- ´Ù¾çÇÑ cell¿¡ DNA / RNA / ´Ü¹éÁú ¸ðµÎ Àû¿ë °¡´ÉÇÑ transfection ½Ã¾à,
Trans
IT-X2 Dynamic Delivery System (Code MIR 6000)
- ³» cell¿¡ ¸Â´Â MirusÀÇ ÃÖÀû transfection ½Ã¾à ã±â online tool,
Reagent Agent
¢ç
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
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Keyword :
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Systems Product
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Lenti-X Tet-On 3G Inducible Expression System
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Linear / Plasmid)
All-in-one vector ÇüÅÂÀÇ Tet-inducible syste..
Tet-One Inducible Expression (One vector System)
TetR Ç×ü
[Legacy] Tetracycline À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ