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Ư¡: Intracellular Lipid ÃàÀûÀÇ ÃøÁ¤

AdipoRed Assay Reagent

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Lonza
PT-7009
AdipoRed Assay Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 x 4ml
°¡°Ý¹®ÀÇ   AdipoRed_Assay_Reagent.pdf

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PreadipocytesÀÇ differentiation¿¡ ´ëÇÑ È­ÇÕ¹°ÀÇ ¿µÇâÀ» Æò°¡Çϱâ À§ÇÑ Á¦Ç°ÀÌ´Ù. Lipophilic AdipoRed Assay Reagent´Â adipocytesÀÇ fat droplet°ú 572 nm¿¡¼­ fluorescesÀ¸·Î ±¸ºÐÇÑ´Ù. Homogeneous fluorescence-based assay´Â intracellular triglycerides ÃàÀûÀÇ Á¤·®À» high-throughputÀ¸·Î ÁøÇàÇÒ ¼ö ÀÖÀ¸¸ç obesity(ºñ¸¸)¿Í diabetes(´ç´¢º´) °ü·Ã ºÐ¾ß¿¡ ´ëÇÑ ½Å¾à ¹ß°ß µî¿¡ »ç¿ë ÇÒ ¼ö ÀÖ´Â ¸Å¿ì ¶Ù¾î³­ ½ÇÇè ¹æ¹ýÀÌ´Ù. ÀÌ kitÀº Oil Red O assay¿Í °°Àº ´Ù¸¥ ¹æ¹ýµé º¸´Ù ¹Î°¨ÇÏ°í Northern blotÀ̳ª Western blot º¸´Ù ´õ ºü¸£°í ´õ °£ÆíÇÏ°Ô ½ÇÇè
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  • Convenient - Simply remove cell culture medium, add premixed reagent and read in a standard fluorometer
  • Fast- Process an entire 96-well or 384-well plate in as little as 20 minutes
  • Effective - Provides objective, high throughput measurement of the accumulation of intracellular triglycerides, with high signal to noise ratios

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Inhibition of Adipocyte Di_erentiation Assayed with AdipoRed Assay Reagent


±×¸²1. Poietics¢â Primary Human Preadipocytes were induced to differentiate in the presence of TNF¥á, Mifepristone or no inhibitor. Lipid accumulation was assayed after 4 days in culture.

Keyword : Áö¹æ,´ç´¢,ºñ¸¸,µ¿¸Æ°æÈ­,°íÇ÷¾Ð

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