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Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > In-Fusion Cloning > [Kit] In-Fusion¢ç EcoDry Cloning

[Kit] In-Fusion¢ç EcoDry Cloning

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
639678
In-Fusion¢ç HD EcoDry Cloning Kit w/Competent Cells
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
8 ȸ
438,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32738, x32739, x33081, x33613
Clontech
639679
In-Fusion¢ç HD EcoDry Cloning Kit w/Competent Cells
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
8 ȸ¡¿3
1,226,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639680
In-Fusion¢ç HD EcoDry Cloning Kit w/Competent Cells
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
96 ȸ (96-well plate)
2,622,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639687
In-Fusion¢ç HD EcoDry Cloning Kit w/NucleoSpin
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ (96-well plate)
2,312,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32738, x32862, x33613
Clontech
639689
In-Fusion¢ç HD EcoDry Cloning Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
8 ȸ
276,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â PT5055-2.pdf, In-Fusion%20HD%20EcoDry%20Multiple-Insert%20Cloning%20Protocol-At-A-Glance_121416.pdf, In-Fusion%20HD%20EcoDry%20Cloning%20Kit%20User%20Manual_080318.pdf
Clontech
639690
In-Fusion¢ç HD EcoDry Cloning Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
8 ȸ¡¿3
745,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
639691
In-Fusion¢ç HD EcoDry Cloning Kit
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96 ȸ(96-well plate)
2,070,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
636763
Stellar¢â Competent Cells
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç° 
10 ȸ
(10 x 100ul)
280,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32785, x33081
Clontech
636767
Stellar¢â Competent Cells (96 well plate)
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç° 
96 ȸ
(96 x 20ul)
760,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33079


¡Ø In-Fusion Cloning »ý»ý Èı⺸±â
PCR Cloning Kits¡ªDNA Cloning¡ªIn Fusion HD Cloning System
In-Fusion Cloning SystemÀº ¾î¶² PCR ´ÜÆíÀ̳ª º¹¼öÀÇ DNA ´ÜÆíµµ ¼±ÇüÈ­µÈ vector(linearized vector)¿¡ 15ºÐ(In-Fusion HD EcoDry´Â 30ºÐ)ÀÇ ¹ÝÀÀÀ¸·Î directional cloningÀÌ °¡´ÉÇÏ´Ù (Figure 1). ¶ÇÇÑ PCR ´ÜÆíÀº Á¦ÇÑÈ¿¼Ò󸮳ª phosphorylation°ú °°Àº Ãß°¡ÀûÀº ó¸® ´Ü°è°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù. In-Fusion È¿¼Ò´Â ¼±ÇüÈ­µÈ vector ¸»´Ü°ú PCR·Î ÁõÆøµÈ PCR ´ÜÆí(insert)ÀÇ ¸»´ÜÀÇ 15bp homologue sequence¸¦ ÀνÄÇÏ¿© À¶ÇÕ½ÃŲ´Ù. ÀÌ 15 bpÀÇ À¶ÇÕ¼­¿­Àº ¿øÇÏ´Â sequence¸¦ ÁõÆøÇϰíÀÚ ÇÏ´Â PCR primer¿¡ µðÀÚÀÎÇÏ¿© ²¸ ³ÖÀ¸¸é ½±°Ô ¸¸µé ¼ö ÀÖ´Ù.

ÀÌ ½ÇÇè¹æ¹ýÀ» »ç¿ëÇÏ¸é ¾Æ·¡¿Í °°Àº ´Ù¾çÇÑ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÏ´Ù.
  • Subcloning ¾øÀ̵µ º¹¼öÀÇ DNA ´ÜÆíÀ» ¿øÇÏ´Â vector¿¡ Cloning
  • ³»ºÎÀÇ Æ¯Á¤ ºÎºÐ¸¸ ±³Ã¼ÇÏ´Â Modular expression vector Á¦ÀÛ
  • ºÒÇÊ¿äÇÑ ¿°±â°¡ ¾ø´Â Fusion protein ±¸Ãà
  • DNA ´ÜÆíÀÇ Á¦°Å³ª ±³Ã¼
  • InsertÀÇ point mutationÀ̳ª Á¦ÇÑÈ¿¼Ò »çÀÌÆ® ÷°¡
  • À¯ÀüÀÚ³» Çü±¤ ´Ü¹éÁúÀÇ tag ±³Ã¼
  • cDNA·Î UTRs÷°¡


Figure 1. The In-Fusion cloning protocol.


Figure 2. The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).


Figure 3. The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

Keyword : infusion,in fusion, ÀÎÇ»,infusion, In-fusion, ÀÎÇ»Á¯, ÀÎÇ»Àü, in fusion, Ŭ·Î´×, cloning, mutagenesis, mutation,soeasy,so easy