[°øÁö] [½ÅÁ¦Ç°] CRISPR: sgRNA Á¦ÀÛ ¹× ½ºÅ©¸®´×_¾÷±×·¹À̵å Ãâ½Ã ¹× º¯°æ °øÁö
´õ¿í È¿À²ÀûÀÌ°í °£ÆíÇÑ CRISPR/Cas9 Guide-it¢â Á¦Ç°À» °³¹ßÇÏ´Â ´ÙÄ«¶ó¹ÙÀÌ¿À°¡ CRISPR/Cas9 ½Å±Ô Á¦Ç° Ãâ½Ã¸¦ ¾È³» µå¸³´Ï´Ù.
±âÁ¸ Á¦Ç°¿¡ ºñÇÏ¿© ´õ¿í ³ôÀº ¼öÀ²°ú °í¼øµµÀÇ sgRNA¸¦ ÇÕ¼ºÀÌ °¡´ÉÇϸç, ÇÕ¼ºÇÑ sgRNA´Â CRISPR/Cas9 genome editing¿¡ Á÷Á¢ ÀÌ¿ë °¡´ÉÇÕ´Ï´Ù.
in vitro transcription¿¡ ÀÇÇÑ sgRNA Á¦ÀÛ ¹× È¿À² ½ºÅ©¸®´×
Guide-it¢â sgRNA In Vitro Transcription and Screening System
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- Ãâ½ÃÀÏ: 2016³â 11¿ù
- ³» ¿ë: ±âÁ¸ÀÇ sgRNA in vitro screening system ÆÇ¸Å Áß´Ü ¹× ¾÷±×·¹ÀÌµå ¹öÀü Ãâ½Ã
- ¸ñ Àû: 1) sgRNA ÇÕ¼º ¼öÀ² Çâ»ó, 2) sgRNA °í¼øµµ Á¤Á¦À² Çâ»ó, 3) CRISPR/Cas9 È¿À² Çâ»ó
- ÁÖ ÀÇ: ±âÁ¸ Á¦Ç°°ú ºñ±³ÇÏ¿© sgRNA ÇÕ¼ºÀ» À§ÇÑ Forward primer ¼¿(scaffold, promoter)ÀÌ º¯ÇüµÇ¾ú½À´Ï´Ù.
ÀÚ¼¼ÇÑ ³»¿ëÀº Á¦Ç°ÀÇ »ó¼¼Á¤º¸ ÆäÀÌÁö È®ÀÎ ¹Ù¶ø´Ï´Ù.
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ü CRISPR/Cas9 genome editingÀ» À§ÇÑ sgRNAÀÇ ÇÕ¼º (in vitro transcription)
ü °£ÆíÇÑ ÇÁ·ÎÅäÄÝ·Î °íǰÁú, °í¼öÀ² (12ug ÀÌ»ó)ÀÇ sgRNA Á¦ÀÛ °¡´É
ü ÇÕ¼ºÇÑ sgRNA´Â ÃßÈÄ delivery (transfection, electroporation)¿¡ ÀÌ¿ë °¡´É
ü Screening Kit¿¡ Æ÷ÇԵǾîÀÖ´Â Cas9 ´Ü¹éÁú°ú Á¶ÇÕÇÏ¿©, ¼¼Æ÷ ½ÇÇè Àü¿¡ sgRNAÀÇ À¯È¿¼º ¹× È¿À² È®ÀÎ
Á¦Á¶»ç |
Á¦Ç°ÄÚµå |
Á¦Ç°¸í |
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Old version code |
Clontech |
632635 |
50ȸ |
631438 | |
Clontech |
632636 |
50ȸ |
631439 | |
Clontech |
632639 |
50ȸ |
631440 | |
Clontech |
632638 |
50ȸ |
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