[5 x premix ŸÀÔÀÇ 1st cDNA ÇÕ¼º kit]
PrimeScript¢â IV 1st strand cDNA Synthesis Mix (Code 6215A/B)
Total RNA ¶Ç´Â PolyA+ RNA·ÎºÎÅÍ 1st cDNA¸¦ ÇÕ¼ºÇϱâ À§ÇÑ 5 x premix ½Ã¾àÀÌ Ãâ½ÃµÇ¾ú½À´Ï´Ù.
º» Á¦Ç°À» ÀÌ¿ëÇϸé cDNA ÇÕ¼º¿¡ ÇÊ¿äÇÑ ¿ªÀü»çÈ¿¼Ò PrimeScript¢â IV RTase, RNase Inhibitor, Oligo dT primer, dNTP mixture ¹× ¹ÝÀÀ buffer°¡
premixµÇ¾î ÀÖ¾î, template RNA¿Í D.W¸¦ ÷°¡ÇÏ´Â °Í ¸¸À¸·Î ½±°í ºü¸£°Ô ½ÇÇèÀ» ÁøÇàÇÒ ¼ö ÀÖ½À´Ï´Ù.
¢º 5 x premix ŸÀÔÀÇ 1st strand cDNA ÇÕ¼º ½Ã¾à
¢º 10~20ºÐÀÇ ¿ªÀü»ç¹ÝÀÀÀ¸·Î long fragment (13 kb) RNA ¿ªÀü»ç °¡´É
¢º Çʿ信 µû¶ó 42 ¡É ¶Ç´Â 52 ¡É ¹ÝÀÀ ÁøÇà
¡á Á¦Ç°±¸¼º - Á¦Ç°»ó¼¼ÆäÀÌÁö (¸µÅ©)
PrimeScript¢â IV 1st strand cDNA Synthesis Mix (Code 6215A, 20 §¡ ¹ÝÀÀ ½Ã 50ȸ ºÐ·®)
1. |
5¡¿ PrimeScript¢â IV cDNA Synthesis Mix *1 |
200 §¡ |
2. |
Random 6 mers (50 ¥ìM) *2 |
100 §¡ |
3. |
RNase Free dH2O |
1 §¢¡¿ 2 |
*1 PrimeScript¢â IV RTase, RNase Inhibitor, Oligo dT Primer, dNTP Mixture ¹× ¹ÝÀÀ buffer (Mg2+ ÇÔÀ¯)À» Æ÷ÇÔÇÑ´Ù.
*2 polyA+À» Æ÷ÇÔÇÏÁö ¾Ê´Â RNAÀÇ cDNA ÇÕ¼º ¶Ç´Â Real Time PCR¿ë cDNA ÇÕ¼º, RNAÀÇ Àü ¿µ¿ªÀ» °í·ç
cDNA ÇÕ¼ºÇÏ´Â °æ¿ì¿¡ ¹ÝÀÀ mixture¿¡ ÷°¡ÇÑ´Ù.
Á¦Ç° °ü·ÃµÈ ¹®ÀÇ»çÇ×Àº °í°´Áö¿ø¼¾ÅÍ (02-2081-2510, support@takara.co.kr) ·Î ¿¬¶ô¹Ù¶ø´Ï´Ù.
°¨»çÇÕ´Ï´Ù.