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Nco I

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
1160A
Nco I
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 U
135,000¿ø  1160A_DS.v1807Da.pdf
Takara
1160B
Nco I
°ü·ÃÇмú ±¸¸ÅÇϱâ
2,500 U (1160A x 5)
605,000¿ø 
Takara
1160BH
Nco I (°í³óµµ)
°ü·ÃÇмú ±¸¸ÅÇϱâ
2,500 U (1160AH x 5)
3,055,000¿ø  1160BH_DS.v1807Da.pdf

º¸Á¸
-20¡É
³óµµ
10 U/§¡ [°í³óµµ: 50 U/§¡]
÷ºÎ ¹× È°¼º ÃøÁ¤ buffer
K buffer + BSA
[Âü°í] Universal Buffer ¡¤ Basal Buffer¿¡ ÀÇÇÑ Á¦ÇÑÈ¿¼Ò È°¼º Ç¥½Ã ½Ã½ºÅÛ
[Âü°í] Double Digestion¿ë Ãßõ Universal Buffer
¹ÝÀÀ ¿Âµµ
37¡É
È°¼ºÀ» ÃøÁ¤ÇÑ ±âÁú
¥ëDNA
±â¿ø
Escherichia coli carrying the plasmid encoding Nco I gene
Star È°¼º
°í³óµµÀÇ ±Û¸®¼¼·Ñ, DMSO, ¶Ç´Â Mn2+°¡ Á¸ÀçÇÒ ¶§, ¼­¿­ ÀÎ½Ä Æ¯À̼ºÀÌ °¨¼ÒµÉ ¼ö ÀÖ´Ù.
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