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Ç×ü ¡¤ ELISA ¡¤ Signal
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Apoptosis
> ApoAlert DNA Fragmentation Assay Kit
ApoAlert DNA Fragmentation Assay Kit
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The ApoAlert DNA Fragmentation Assay Kit detects nuclear DNA fragmentation, a hallmark of apoptosis. The kit can be used with tissue sections as well as cultured cells, and results can be visualized directly by fluorescence microscopy or quantified by flow cytometry.
During apoptosis, cellular endonucleases cleave nuclear DNA between nucleosomes, producing a mix of DNA fragments whose length varies in multiples of 180 to 200 bp. The ApoAlert DNA fragmentation assay incorporates fluorescein- dUTP at the free 3'-hydroxyl ends of the fragmented DNA using the TUNEL (terminal deoxynucleotidyl transferase- mediated dUTP nick-end-labeling) method (1). The fluorescein-labeled DNA can then be quantified using either fluorescence microscopy or flow cytometry.
±×¸²1.DNA fragmentation in tissue sections.
Mouse embryo (day E14) tissue was fresh-frozen, cut into sections, and mounted on slides by standard methods. The ApoAlert DNA Fragmentation Assay was performed according to the User Manual. Sections were photographed using a fluorescence microscope with either a propidium iodide (PI) filter alone (Panel A), a FITC filter alone (Panel B), or a dual-pass FITC/PI filter set (Panel C). Apoptotic cells appear green with the FITC filter alone. Apoptotic cells appear yellow and nonapoptotic cells appear red under the dual-pass FITC/PI filter set.
±×¸² 2. Flow cytometry analysis of DNA fragmentation in apoptotic cells in suspension.
Jurkat cells were grown in RPMI medium + 10% FBS. Apoptosis was induced by treatment with 200 ng/ml anti-FAS mAb for 15.5 hr. The ApoAlert DNA fragmentation assay was performed according to the User Manual. Cells were analyzed by flow cytometry. Panel A. Uninduced cells. Panel B. Induced cells.
Components
-Equilibration Buffer
-Nucleotide Mix
-Terminal Deoxynucleotidyl Transferase (TdT)
-SSC
-Proteinase K
-Plastic Coverslips
-User Manual (PT3137-1)
References
1. Gavrieli, Y., et al. (1992) J. Cell. Biol. 119(3):493-501.
Product index
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TaKaRa DAB Substrate
TaKaRa POD Conjugate½Ã..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã Ç×..
½Å°æ¹ß»ýºÐÈ ¸¶Ä¿ °ü·Ã..
EIA Kit
Acrolein-Lysine Adduct..
Fibronectin EIA Kit
Human Albumin EIA Kit
Human IgG EIA Kit
Human Vitronectin EIA ..
Human/Pig Osteonectin ..
Laminin(LN) EIA Kit
Mouse Albumin EIA Kit
Mouse Gla-Osteocalcin ..
Osteocalcin EIA Kit
Peptide Coating Kit
Pig Osteocalcin EIA Ki..
Procollagen Type I C-p..
Rat Gla-Osteocalcin Hi..
Rat Heme Oxygenase-1 E..
RetroNectin¢ç Á¤·® kit
Universal Tyrosine Kin..
Wash and Stop Solution..
Osteo¤ýAdipo assay
AdipoRed Assay Reagent
OsteoAssay Human Bone ..
Osteoblast-Inducer Rea..
OsteoImage Mineralizat..
OsteoLyse Assay Kit£¨H..
PDELight HTS cAMP Phos..
PPiLight Inorganic Pyr..
TRACP & ALP Assay Kit
TRACP & ALP double-sta..
¼¼Æ÷Áõ½Ä ¡¤ ¼¼Æ÷ÀåÇØ ÃøÁ¤
LDH Cytotoxicity Detec..
Premix WST-1 Cell Prol..
DNA ¡¤ IdU Labeling an..
LDH Cytotoxicity Detec..
Luminescent Assay Acce..
Premix WST-1 Cell Prol..
ToxiLight BioAssay Kit
ViaLight ½Ã¸®Áî
È¿¼ÒȰ¼º ÃøÁ¤ ¡¤ °ËÃâ¿ë Ki..
Heparan Degrading Enzy..
Mouse Section Slide (n..
TRACP & ALP Assay Kit
TRACP & ALP double-sta..
¥â-Galactosidase Stain..
Apoptosis
ApoAlert Annexin V-FIT..
ApoAlert Caspase Assay..
ApoAlert Cell Fraction..
ApoAlert DNA Fragmenta..
ApoAlert pDsRed2-Bid V..
ApopLadder Ex¢â
ApoPrimer Set (Bcl-2 f..
In situ Apoptosis Dete..
Signal Transduction
Dominant-Negative Vect..
ExpressHyb Hybridizati..
Signal Àü´Þ °æ·Î Profi..
TransFactor ÈÇй߱¤ K..
TransFactor Kit£¨¹ß»ö..
TransFactor Universal ..
Ç×ü
Ç×ü ±³Â÷ ¹ÝÀÀ À϶÷Ç¥
Ç×ü ¿ëµµ À϶÷Ç¥
Anti-Albumin
Anti-Bovine Osteocalci..
Anti-Bromodeoxyuridine
Anti-CD3 mAb GMP grade
Anti-Chicken N-cadheri..
Anti-Heme Oxygenase-1
Anti-Human Ago2 (Argon..
Anti-Human Calpastatin
Anti-Human E-cadherin
Anti-Human Fibronectin
Anti-Human Influenza V..
Anti-Human Insulin
Anti-Human iPS
Anti-Human Laminin
Anti-Human N-cadherin
Anti-Human Osteocalcin
Anti-Human P-cadherin
Anti-Human Platelet GM..
Anti-Human Platelet GP..
Anti-Human Procollagen..
Anti-Human Vitronectin
Anti-Human von Willebr..
Anti-Insulin C
Anti-Mouse E-cadherin
Anti-Mouse Osteocalcin
Anti-Mouse P-cadherin
Anti-Osteonectin/SPARC
Anti-ProS2
Anti-Rat Collagen type..
Anti-Rat Osteocalcin
Monoclonal 2nd antibod..
Polyclonal Anti-Dentin..
Polyclonal Anti-Glucag..
Polyclonal Anti-Glycer..
Polyclonal Anti-Human ..
Polyclonal Anti-Human ..
Polyclonal Anti-Human ..
Polyclonal Anti-Mouse ..
Polyclonal Anti-N-cadh..
Polyclonal Anti-Rat Bo..
Polyclonal Anti-Tartra..
Procalcitonin EIA Kit
Áø±Õ Ç׿ø °ü·Ã Á¦Ç°
Anti-Mal f2
Anti-SAP2
Microarray °ü·Ã
Ç×ü array 380 & 500
Glass Hybridization Ch..
Glass_Microarray-Appro..
Keyword :
Apoptosis
ApoAlert Annexin V-FITC Apoptosis Kit
ApoAlert Caspase Assay Kit
ApoAlert Cell Fractionation
ApoAlert DNA Fragmentation Assay Kit
ApoAlert pDsRed2-Bid Vector
Apoptosis·Î ÀÎÇÑ ´ÜÆíÈ DNA °ËÃâ
ApopLadder Ex¢â
Bcl-2 À¯ÀüÀÚ RT-PCR¿¡
ApoPrimer Set (Bcl-2 family)
TUNEL¹ýÀ» ÀÌ¿ëÇÑ Apoptosis °ËÃâ
In situ Apoptosis Detection Kit