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Home > 전제품보기 > 항체 · ELISA · Signal > Apoptosis > ApoAlert DNA Fragmentation Assay Kit

ApoAlert DNA Fragmentation Assay Kit

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The ApoAlert DNA Fragmentation Assay Kit detects nuclear DNA fragmentation, a hallmark of apoptosis. The kit can be used with tissue sections as well as cultured cells, and results can be visualized directly by fluorescence microscopy or quantified by flow cytometry.

During apoptosis, cellular endonucleases cleave nuclear DNA between nucleosomes, producing a mix of DNA fragments whose length varies in multiples of 180 to 200 bp. The ApoAlert DNA fragmentation assay incorporates fluorescein- dUTP at the free 3'-hydroxyl ends of the fragmented DNA using the TUNEL (terminal deoxynucleotidyl transferase- mediated dUTP nick-end-labeling) method (1). The fluorescein-labeled DNA can then be quantified using either fluorescence microscopy or flow cytometry.
그림1.DNA fragmentation in tissue sections. Mouse embryo (day E14) tissue was fresh-frozen, cut into sections, and mounted on slides by standard methods. The ApoAlert DNA Fragmentation Assay was performed according to the User Manual. Sections were photographed using a fluorescence microscope with either a propidium iodide (PI) filter alone (Panel A), a FITC filter alone (Panel B), or a dual-pass FITC/PI filter set (Panel C). Apoptotic cells appear green with the FITC filter alone. Apoptotic cells appear yellow and nonapoptotic cells appear red under the dual-pass FITC/PI filter set.
그림 2. Flow cytometry analysis of DNA fragmentation in apoptotic cells in suspension. Jurkat cells were grown in RPMI medium + 10% FBS. Apoptosis was induced by treatment with 200 ng/ml anti-FAS mAb for 15.5 hr. The ApoAlert DNA fragmentation assay was performed according to the User Manual. Cells were analyzed by flow cytometry. Panel A. Uninduced cells. Panel B. Induced cells.
Components
-Equilibration Buffer
-Nucleotide Mix
-Terminal Deoxynucleotidyl Transferase (TdT)
-SSC
-Proteinase K
-Plastic Coverslips
-User Manual (PT3137-1)
References
1. Gavrieli, Y., et al. (1992) J. Cell. Biol. 119(3):493-501.

Keyword :
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Apoptosis
> ApoAlert Annexin V-FITC Apoptosis Kit
> ApoAlert Caspase Assay Kit
> ApoAlert Cell Fractionation
> ApoAlert DNA Fragmentation Assay Kit
> ApoAlert pDsRed2-Bid Vector
Apoptosis로 인한 단편화 DNA 검출
> ApopLadder Ex™
Bcl-2 유전자 RT-PCR에
> ApoPrimer Set (Bcl-2 family)
TUNEL법을 이용한 Apoptosis 검출
> In situ Apoptosis Detection Kit
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