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Home > ÀüÁ¦Ç°º¸±â > Ç×ü ¡¤ ELISA ¡¤ Signal > Apoptosis > In situ Apoptosis Detection Kit
TUNEL¹ýÀ» ÀÌ¿ëÇÑ Apoptosis °ËÃâ

In situ Apoptosis Detection Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
MK500
in situ Apoptosis Detection Kit
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
20 ȸ
377,000¿ø  MK500_e.v1609Da.pdf, MK500_e.v1005Da.pdf
Takara
MK501
Labeling Safe Buffer
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
500 ¥ìl¡¿10
772,000¿ø 
Takara
MK502
TdT Enzyme
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
50 ¥ìl¡¿10
356,000¿ø 
Takara
MK503
Anti-FITC HRP Conjugate
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
1.5 ml¡¿5
326,000¿ø 
Takara
MK504
Control Slides
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
2 Slides¡¿5
157,000¿ø 
Takara
MK505
Permeabilisation Buffer
°ü·ÃÇмú °ü½É»óǰµî·Ï ±¸¸ÅÇϱâ
1.0 ml¡¿10
132,000¿ø 

³»¿ë (20ȸ)
1. Labeling Safe Buffer 500 §¡¡¿2 vials
2. TdT Enzyme 50 §¡¡¿2 vials
3. Anti-FITC HRP Conjugate
4. Control Slides 2 slides
5. Permeabilisation Buffer 1.0 §¢¡¿2 vials
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1, 2, 5: -20¡É
3: 4¡É (¼ö¼Û ½Ã´Â -20¡É. Çѹø À¶ÇØÇÑ ÈÄ¿¡´Â 4¡É¿¡ º¸Á¸)
4: ½Ç¿Â (¼ö¼Û ½Ã´Â -20¡É)
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º» ŰƮ´Â apoptosis¸¦ ÀÏÀ¸Å°´Â ¼¼Æ÷ÀÇ Æ¯Â¡ Áß ÇϳªÀÎ ´ÜÆíÈ­µÈ chromatin DNA¸¦ ¸»´ÜÇ¥½Ä¹ý¿¡ ÀÇÇØ Á¶Á÷È­ÇÐÀûÀ¸·Î °ËÃâÇϴ ŰƮÀÌ´Ù. In situ¿¡¼­ Ç¥½ÄÇÙ»êÀÇ ÇÔÀÔ (incorporation)À¸·Î apoptosisÀÇ Á¶Á÷ÇÐÀû ºÐÆ÷³ª °¢°¢ÀÇ ¼¼Æ÷¸¦ È¿À²ÀûÀ¸·Î °ËÃâÇÏ´Â TUNEL (TdT-mediated dUTP-biotin nick end labeling)¹ýÀ» ÀÌ¿ëÇÏ¿© Á¶Á÷´ÜÆí, °íÁ¤¼¼Æ÷ µîÀÇ ¾î¶² ½Ã·á¿¡µµ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç Çü±¤Çö¹Ì°æ, ±¤ÇÐÇö¹Ì°æ, flow cytometry·Îµµ °üÂûÀÌ °¡´ÉÇÏ´Ù.
º» ŰƮ¿¡´Â ¸é¿ª¹ÝÀÀ¿ë ±âÁúÀ̳ª ¿°»ö¿ë ½Ã¾àÀº Æ÷ÇԵǾî ÀÖÁö ¾Ê´Ù. Control Slide´Â ·¡Æ®(rat) À¯¼±Á¶Á÷ÀÇ ÆÄ¶óÇÉ (paraffin) Æ÷¸Å´ÜÆíÀ̸ç, Positive Control Slide·Î »ç¿ëÇÒ ¶§´Â ÆÄ¶óÇÉÀ» Á¦°ÅÇÑ ÈÄ¿¡ ÷ºÎÇÑ »ç¿ë¼³¸í¼­ÀÇ ¡¸Á¶ÀÛ¼ø¼­¡¹ (ÆÄ¶óÇÉ Æ÷¸Å´ÜÆíÀÇ °æ¿ì)¿¡ µû¶ó »ç¿ëÇÑ´Ù.
Å»ÆÄ¶óÇɰú Proteinase K¸¦ ó¸®ÇÑ ÈÄ¿¡´Â °üÂû¹æ¹ý¿¡ µû¶ó ÀûÀýÇÑ ÇÁ·ÎÅäÄÝ·Î Á¶ÀÛÇÑ´Ù.
ŰƮÀÇ component´Â ´Üǰ (Bulk)À¸·Îµµ ÆÇ¸ÅÇÑ´Ù.
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TUNEL¹ýÀº apoptosis¸¦ ÀÏÀ¸Å² ¼¼Æ÷¿¡¼­ ´ÜÆíÈ­µÈ DNAÀÇ À¯¸® 3¡¯-OH ¸»´ÜÀ» terminal transferase (TdT)·Î fluorescein-dUTP¸¦ °íÈ¿À²ÀûÀ̸鼭µµ ƯÀÌÀûÀ¸·Î Ç¥½ÄÇÑ ÈÄ Çü±¤Çö¹Ì°æÀ̳ª flow cytometry·Î °ËÃâÇÏ´Â ¹æ¹ýÀÌ´Ù. ÇÔÀÔµÈ fluoresceinÀº peroxidase Ç¥½Ä anti-fluorescein Ç×ü¸¦ ÀÌ¿ëÇÑ ¹ß»ö¹ÝÀÀÀ» ÅëÇÏ¿© ±¤ÇÐÇö¹Ì°æÀ¸·Îµµ °üÂûÇÒ ¼ö ÀÖ´Ù.
Bulk ´Üǰ Component
Labeling Safe Buffer MK501 500 §¡¡¿10 706,000¿ø
TdT Enzyme MK502 50 §¡¡¿10 325,000¿ø
Anti-FITC HRP Conjugate MK503 1.5 §¢¡¿5 297,000¿ø
Control Slides MK504 2 slides¡¿5 143,000¿ø
Permeabilisation Buffer MK505 1.0 §¢¡¿10 121,000¿ø






Keyword :
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Apoptosis
> ApoAlert Annexin V-FITC Apoptosis Kit
> ApoAlert Caspase Assay Kit
> ApoAlert Cell Fractionation
> ApoAlert DNA Fragmentation Assay Kit
> ApoAlert pDsRed2-Bid Vector
Apoptosis·Î ÀÎÇÑ ´ÜÆíÈ­ DNA °ËÃâ
> ApopLadder Ex¢â
Bcl-2 À¯ÀüÀÚ RT-PCR¿¡
> ApoPrimer Set (Bcl-2 family)
TUNEL¹ýÀ» ÀÌ¿ëÇÑ Apoptosis °ËÃâ
> In situ Apoptosis Detection Kit
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