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Gene transfer (virus)
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Lentivirus
> Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â ½Ã½ºÅÛ
Tet-on inducible Expression (Two vectors)
Á¦Á¶»ç
Á¦Ç°ÄÚµå
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(ºÎ°¡¼¼º°µµ)
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Clontech
631187
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System
each
3,140,000¿ø
,
,
,
,
,
Clontech
631363
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha Version)
Each
3,253,000¿ø
,
,
,
,
Clontech
631351
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (Bicistronic Version)
each
2,967,000¿ø
,
,
,
,
,
,
Clontech
631349
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (with mCherry)
each
2,967,000¿ø
,
,
,
,
,
,
Clontech
631350
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (with ZsGreen1)
each
2,967,000¿ø
,
,
,
,
,
,
Clontech
631354
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha, Bicistronic)
each
3,080,000¿ø
,
,
,
,
,
,
Clontech
631352
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha, mCherry)
each
3,080,000¿ø
,
,
,
,
,
,
Clontech
631353
Lenti-X¢â Tet-On¢ç 3G Inducible Expression System (EF1alpha, ZsGreen1)
each
3,080,000¿ø
,
,
,
,
,
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
Titration Kit (qPCR)
Extraction Solution
Lentivirus
Lenti-X ¼±Åð¡À̵å
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
EF1 ¥á promoter º¤ÅÍ ..
Tet-on inducible Expre..
Tet One Inducible Expr..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Á¤È®ÇÑ ¿ª°¡ ÃøÁ¤
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
iPS Cell Generation¢â ..
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
RetroNectin¢ç (Recombi..
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
Ư¡
³·Àº ¹é±×¶ó¿îµå, ³ôÀº À¯µµ¹ßÇö È¿À²
Lenti-X¢â Packaging Single Shots (VSV-G)
Æ÷ÇÔ: °íÈ¿À², ¾ÈÁ¤¼º ³ôÀº ·»Æ¼¹ÙÀÌ·¯½º ÆÐŰ¡ ¿ëÀÌ
TRE3G promote·Î Á¤¹ÐÇÏ°Ô À¯ÀüÀÚ¹ßÇö Á¶Àý
Applications
ÇüÁúÀüȯÀÌ ¾î·Á¿î cell-type¿¡¼ tetracycline À¯µµ ¹ßÇö
Tet-On
¢ç
Inducible Expression - The 3rd Generation
Tet-On
¢ç
3G Tetracycline Inducible Gene Expression SystemsÀº °¡Àå °·ÂÇϰí Àû¿ë¼ºÀÌ ¶Ù¾î³ 3¼¼´ë Æ÷À¯·ù À¯µµ ¹ßÇö ½Ã½ºÅÛÀ¸·Î Á¤¹ÐÇÏ°Ô À¯ÀüÀÚ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö ÀÖµµ·Ï ±âÁ¸ Á¦Ç°ÀÇ ¼º´ÉÀ» °³¼±ÇÏ¿´´Ù. º» ½Ã½ºÅÛÀ» ÀÌ¿ëÇϸé Á» ´õ °¡¿ªÀûÀ̰í Á¤·®ÀûÀ̸ç ÀçÇö¼º ÀÖ°Ô ¸ñÀû À¯ÀüÀÚÀÇ ¹ßÇöÀ» Á¶ÀýÇÒ ¼ö ÀÖ´Ù. Hermann Bujard¿Í Manfred Gossen, Wolfgang Hillen ¿¬±¸ ±×·ì(1,2)¿¡ ÀÇÇØ °³¹ßµÈ 3G ½Ã½ºÅÛÀº promoter º¯ÇüÀ¸·Î ¹é±×¶ó¿îµå¸¦ ÇöÀúÇÏ°Ô ÁÙÀ̰í transactivator protein °³¼±À» ÅëÇØ Dox¿¡ ´ëÇÑ ¹Î°¨µµ¸¦ ÇÑÃþ ³ôÀÎ Çõ½ÅÀûÀÎ Tet ½Ã½ºÅÛÀÌ´Ù.
Tet-On
¢ç
3G Inducible SystemÀÇ °³¿ä
Tet-On
¢ç
3G transactivator proteinÀ» ¹ßÇöÇÏ¸é¼ TRE3G promoter (PTRE3G)·Î ¸ñÀû À¯ÀüÀÚ¸¦ Á¶ÀýÇÏ´Â Tet-On
¢ç
3G ¼¼Æ÷´Â µ¶½Ã»çÀÌŬ¸°(Dox; tetracycline analog)ÀÌ Ã·°¡µÇ¾úÀ» ¶§ ¸ñÀû À¯ÀüÀÚ¸¦ °í¹ßÇö ½ÃŲ´Ù. Tet-On
¢ç
3G proteinÀº Dox°¡ °áÇÕÇϸé ÇüÅÂÀûÀÎ º¯È°¡ »ý°Ü PTRE3G ³»ÀÇ tet operator (tet O)¿¡ °áÇÕÇÏ°Ô µÈ´Ù (±×¸² 1). »õ·Î¿î ½Ã½ºÅÛÀº ±âÁ¸ÀÇ ³·Àº ¼öÁØÀÇ TetR-based system (Clontech¿¡¼´Â ÆÇ¸ÅÇÏÁö ¾Ê´Â Á¦Ç°)°ú´Â ´Þ¸®, Tet-On
¢ç
±â¼úÀ» Àû¿ëÇÏ¿© transcriptionÀ» ÀúÇØÇϱ⠺¸´Ù´Â ÃËÁø½ÃŰ´Â °ÍÀ¸·Î ±Øµµ·Î ³·Àº ±âÀú¹ßÇö°ú ³ôÀº ¸ñÀû À¯ÀüÀÚ ¹ßÇöÀÌ °¡´ÉÇÏ´Ù. »Ó¸¸ ¾Æ´Ï¶ó ÇÑÃþ ´õ »¡¶óÁø ¹ÝÀÀ½Ã°£ µîÀÇ ÀåÁ¡À» °®°í ÀÖÀ¸¸ç ƯÈ÷ in vivo ½ÇÇè¿¡ ÃÖÀûÀÌ´Ù.
±×¸² 1. The Tet-On
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3G Systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G Promoter.
±âº»ÀûÀÎ Á¦Ç° ±¸¼ºÇ°
General System Components
10ug regulator vector
10ug response vector
10ug pLVX-TRE3G-Luc Control Vector
16rxns Lenti-X¢â Packaging Single shots (VSV-G)
3rxns Lenti-X¢â GoStix Plus
Keyword :
Lentivirus
Lenti-X ¼±Åð¡À̵å
[°³¿ä] Packaging Single Shots
[FAQ] Lentiviral Gene Delivery
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
Pormoterless vector·Î pomorter Ȱ¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
4½Ã°£ ¸¸¿¡ LentivirusÀÇ ¿ª°¡¸¦ Á¤È®ÇÏ°Ô ÃøÁ¤..
Á¤È®ÇÑ ¿ª°¡ ÃøÁ¤
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
ÃÖÀûÈµÈ Transfection Reagent & Packaging Mi..
Trans
IT¢ç Lentivirus System