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4½Ã°£ ¸¸¿¡ LentivirusÀÇ ¿ª°¡¸¦ Á¤È®ÇÏ°Ô ÃøÁ¤
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Clontech
631235
Lenti-X¢â qRT-PCR Titration Kit
200 ȸ
927,000¿ø
,
,
Clontech
632200
Lenti-X¢â p24 Rapid Titer Kit
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HIV-1 ±â¹ÝÀÇ Lentivirus ¿ª°¡¸¦ ½Å¼ÓÇϰí Á¤È®ÇÏ°Ô Á¤·®
4½Ã°£ ¸¸¿¡ Á¤È®ÇÏ°Ô Lentivirus genome copy¸¦ ÃøÁ¤ÇÏ´Â qRT-PCR Kit
HIV-1 p24 capsid proteinÀ» ELISA ¹æ½ÄÀ¸·Î ¿ª°¡ ÃøÁ¤ÇÏ´Â p24 Rapid Titer Kit
Virus ¿ª°¡ ÃøÁ¤ÀÌ ÇÊ¿äÇÑ ÀÌÀ¯
1. Lentivirus Packaging ¹ÝÀÀÀÌ Á¦´ë·Î µÇ¾ú´ÂÁö È®ÀÎ
2. ¿ÀÂ÷¾øÀÌ ÀϰüµÈ ½ÇÇè°á°ú
3. 100% TransductionÀ» À§ÇÑ ÃæºÐÇÑ MOI °áÁ¤
4. Copy number Á¶ÀýÀ» ÅëÇÑ ´Ü¹éÁú ¹ßÇö¾ç Á¶Àý
Lenti-X¢â qRT-PCR Titration Kit (Code 631235)
Lenti-X¢â qRT-PCR Titration Kit´Â °£´ÜÇÏ°í ºü¸¥ Real Time PCR ¹æ¹ýÀ» ÀÌ¿ëÇÏ¿© Lentivirus ¿ª°¡¸¦ ÃøÁ¤ÇÏ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº Real Time PCRÀ» ÀÌ¿ëÇÏ¿© Lentiviral genome copy¸¦ ÃøÁ¤ÇÏ´Â °ÍÀº ¹°·Ð, ½Å¼ÓÇÑ RNA Á¤Á¦ °úÁ¤À» Æ÷ÇÔÇϰí ÀÖ´Ù.
Ư¡ 1. Rapid determination of lentiviral titer
ÀÌÀü¿¡ »ç¿ëµÇ¾î¿Ô´ø Lentivirus ¿ª°¡ ÃøÁ¤ ¹æ¹ýÀÌ ¾à 10ÀÏ Á¤µµ°¡ ¼Ò¿äµÇ¾ú´ø ¹Ý¸é, Real Time PCRÀ» »ç¿ëÇÏ´Â º» Á¦Ç°À» ÀÌ¿ëÇÏ¸é ¸ðµç HIV-1 ±â¹ÝÀÇ Lentivirus vector¸¦ ¾à 4½Ã°£ ¸¸¿¡ Á¤·®ÇÒ ¼ö ÀÖ´Ù. qRT-PCR ¹æ¹ýÀº ¹ÙÀÌ·¯½º ȸ¼ö (virus harvest)¿Í ¸ñÀû¼¼Æ÷ °¨¿° (target cell infection) ±îÁöÀÇ ½Ã°£À» ȹ±âÀûÀ¸·Î °¨¼Ò½Ãų ¼ö ÀÖ¾î, ¹ÙÀÌ·¯½º ³óÃà, ¿ª°¡ ÃøÁ¤, °¨¿°±îÁö ÇÏ·ç¿¡ ¿Ï·áÇÒ ¼ö ÀÖ´Ù.
Ư¡ 2. Achieve consistent lentiviral transductions
Lentiviral gene-deliveryÀÇ ÁÖ¿äÇÑ ÀåÁ¡Àº ¸ñÀû¼¼Æ÷¿¡ µµÀԵǴ viral genome number¸¦ ¿¹Ãø°¡´ÉÇÑ Multiplicity of infection (MOI)¸¦ ¿¹ÃøÇÔÀ¸·Î½á À¯ÀüÀÚ ¹ßÇöÀ» Á¶Àý °¡´ÉÇÏ´Ù´Â Á¡ÀÌ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇϸé Lentivirus gene copy number¿Í lentiviral infectivity »çÀÌÀÇ »ó°ü°ü°è¸¦ ºü¸£°Ô °áÁ¤ÇÒ ¼ö Àֱ⠶§¹®¿¡, ½ÇÇè°£ÀÇ ¸ñÀû À¯ÀüÀÚ ¹ßÇöÀ» º¸´Ù ÀϰüµÇ°Ô Á¶Àý °¡´ÉÇÏ´Ù.
* º» Á¦Ç° ³»¿¡ Æ÷ÇÔµÈ primer´Â Packaging Signal¿¡ ÀÎÁ¢ÇÑ HIV-1 genome ¿µ¿ªÀ» ÁõÆøÇÒ ¼ö ÀÖµµ·Ï µðÀÚÀεǾî ÀÖ½À´Ï´Ù. º» ÇÁ¶óÀ̸Ӵ ´ÙÄ«¶ó¹ÙÀÌ¿À¿¡¼ ÆÇ¸ÅÇÏ´Â ¸ðµç Lentivirus vector »Ó¸¸ ¾Æ´Ï¶ó ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â HIV-1 based lentiviral vector¿¡ ȣȯ °¡´ÉÇÕ´Ï´Ù. ¸¸¾à, vector compatibilty¸¦ È®ÀÎÇϰíÀÚ ÇÑ´Ù¸é, °í°´Áö¿ø¼¾ÅÍ (TEL. 02-2081-2510)·Î ¿¬¶ô ¹Ù¶ø´Ï´Ù.
±×¸² 1. Lenti-X and Retro-X qRT-PCR kitration kits offer the fastest titration methods.
Standard transduction-based titration methods require up to 10 days to complete.The Lenti-X and Retro-X qRT-PCR titration kits require only 4 hr, which enables virus harvest, titration, and infection of target cells to all be performed on the same day.
±×¸² 2. Flowchart of the procedures for titrating lentivirus supernatants using the Lenti-X qRT-PCR Titration Kit.
Lenti-X¢â p24 Rapid Titer Kit (Code 632200)
Lenti-X¢â p24 Rapid Titer Kit´Â ¹ÙÀÌ·¯½º »óÃþ¾× ³»¿¡ Á¸ÀçÇÏ´Â p24 capsid proteinÀÇ ÀÜÁ¸·®À» ÃøÁ¤Çϴ ƯÀ̼º ³ôÀº ELISA¹ýÀ» ÀÌ¿ëÇÑ´Ù. ÀÌ ¶§ ÃøÁ¤ÇÏ´Â p24 capside proteinÀº ¹ÙÀÌ·¯½º ¿ª°¡¿Í Á÷Á¢ÀûÀÎ »ó°ü°ü°è°¡ ÀÖ´Ù. »óÃþ¾×¿¡ Á¸ÀçÇÏ´Â ¹ÙÀÌ·¯½º´Â Á¦Ç° ³» Æ÷ÇÔµÈ lysis buffer·Î ¿ëÇØÇÑ´Ù. ±× ÈÄ, murine anti-p24 antibody°¡ ÄÚÆÃµÈ 96-well microtiter plate¿¡ ¿ëÇØµÈ »ùÇÃÀ» ó¸® ¹× washingÀ» ÁøÇàÇÑ´Ù. ƯÀÌÀûÀ¸·Î °áÇÕµÈ p24 proteinÀ» biotinylated anti-p24 secondary antibody, streptavidine-HRP (horseradish peroxidase) ¹× ¹ß»ö ½Ã¾àÀ¸·Î ¹ÝÀÀ½ÃŲ´Ù. º» Á¦Ç°Àº standard curve¸¦ ÀÛ¼ºÇÒ ¼ö ÀÖ´Â p24 controlÀ» Æ÷ÇÔÇϰí ÀÖ´Ù.
±×¸² 3. Schematic of the Lenti-X p24 Rapid Titer Procedure.
p24 from the culture supernatant is lysed and bound to anti-p24 coated wells and detected using a combination of biotinylated anti-p24 secondary antibody, streptavidin-HRP, and a color reagent.
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
Lenti-X¢â qRT-PCR Titration Kit (Code 631235)
Lenti-X qRT-PCR Titration Components
NucleoSpin RNA Virus Kit
Quant-X One-Step qRT-PCR TB Green Kit
Lenti-X¢â p24 Rapid Titer Kit (Code 632200)
Anti-p24 Coated Plate (96 wells)
p24 Control (10ng/ml)
Lysis Buffer
Anti-p24 (Biotin Conjugate)
Streptavidine HRP
Wash Buffer (20X Concentrate)
TMB Substrate
Stop Solution
°ü·ÃÁ¦Ç°
´Ü 5ºÐ, Instant lentivirus Á¤·®:
Lenti-X¢â GoStix¢â Plus (Code 631280)
¹ÙÀÌ·¯½º ³óµµ°¡ ³·À» ¶§, ³óÃà:
Lenti-X¢â Concentrator (Code 631231)
Provirus Á¤·®:
Lenti-X¢â Provirus Quantitation Kit (Code 631239)
Keyword : Lentivirus,·»Æ¼¹ÙÀÌ·¯½º,¿ª°¡,¿ª°¡ÃøÁ¤,Titer,Titration,ŸÀÌÅÍ,Á¤·®,¹ÙÀÌ·¯½º Á¤·®,¹ÙÀÌ·¯½º ·»Æ¼,Lenti
Lentivirus
Lenti-X ¼±Åð¡À̵å
[°³¿ä] Packaging Single Shots
[FAQ] Lentiviral Gene Delivery
Lentiviral expression vector¸¦ Æ÷ÇÔÇÑ ½Ã½ºÅÛ..
CMV promoter º¤ÅÍ ½Ã½ºÅÛ
Á¶Ç÷¸ð¼¼Æ÷, Áٱ⼼Æ÷ µîÀÇ Lentiviral transdu..
EF1 ¥á promoter º¤ÅÍ ½Ã½ºÅÛ
SARS-CoV-2-pseudotyped lentivirus »ý»ê
Lenti-X¢â SARS-CoV-2 Packaging Mix
´Ù¾çÇÑ Çü±¤´Ü¹éÁú ¸®Æ÷Å͸¦ Æ÷ÇÔÇÑ Lentiviral..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet-on inducible Expression (Two vectors)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
Tet One Inducible Expression (One vector)
Tetracycline Á¸Àç ÇÏ¿¡¼¸¸ ¸ñÀû ´Ü¹éÁúÀ» ¹ß..
(Legacy) Tet-Inducible expression ½Ã½ºÅÛ
Pormoterless vector·Î pomorter Ȱ¼º ¿¬±¸
Reporter assay ½Ã½ºÅÛ (Promoterless vector)
Transfection ½Ã¾àÀ» Æ÷ÇÔÇÏ¿© ´õ °£´ÜÇÑ packa..
Packaging single shots
239T, HEK-293 Cellº¸´Ù ´õ ³ôÀº packaging È¿..
Packaging Cell Lines
4½Ã°£ ¸¸¿¡ LentivirusÀÇ ¿ª°¡¸¦ Á¤È®ÇÏ°Ô ÃøÁ¤..
Á¤È®ÇÑ ¿ª°¡ ÃøÁ¤
½º¸¶Æ®ÆùÀ¸·Î 10ºÐ¸¸¿¡ Lentivirus ¿ª°¡(Titer)..
GoStix¢â Plus
Lentivirus ³óµµ°¡ ³·À» ¶§, Mix & SpinÀ¸·Î °£..
Lentivirus ³óÃà ½Ã¾à
In vivo Lentiviral delivery Àû¿ë ½Ã, ÇʼöÀû..
Lentivirus Á¤Á¦
Ecotropic lentivirus¸¦ human cell¿¡ Àû¿ë
Ecotropic Receptor Booster
Lentivirus³ª MMLV retrovirusÀÇ ´õ ºü¸¥ trans..
Transduction ½Ã¾à
¸ñÀû ¼¼Æ÷ÀÇ À¯ÀüÀÚ¿¡ »ðÀÔµÈ Provirus copy nu..
Provirus Quantitation Kit
Lentivirus°¡ ¸ñÀû¼¼Æ÷ À¯ÀüÀÚ¿¡ »ðÀÔµÈ À§Ä¡¸¦..
Integration Site Analysis Kit
ÃÖÀûÈµÈ Transfection Reagent & Packaging Mi..
Trans
IT¢ç Lentivirus System