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Home > 전제품보기 > 단백질 발현 · 정제 > His-tag 융합 단백질 정제 > His60 Ni Superflow Cartridges & Gravity Columns
His60 Ni resin 충진제 Pre-packed typ

His60 Ni Superflow Cartridges & Gravity Columns

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Clontech
635675
His60 Ni Superflow Cartridges
관련학술 관심상품등록 구매하기
1 ml×5
326,400원 
408,000원
가격할인
11.01 ~ 12.13
제조사 페이지로 바로가기
His60 Ni Superflow Cartridges User Manual (PT5143-1)_031716.pdf
Clontech
635680
His60 Ni Superflow Cartridges
관련학술 관심상품등록 구매하기
5 ml
196,800원 
246,000원
가격할인
11.01 ~ 12.13
제조사 페이지로 바로가기
Clontech
635679
His60 Ni Superflow Cartridges
관련학술 관심상품등록 구매하기
5 ml×5
837,600원 
1,047,000원
가격할인
11.01 ~ 12.13
제조사 페이지로 바로가기
Clontech
635674
His60 Ni Superflow Cartridge Purification Kit
관련학술 관심상품등록 구매하기
1 ml×5
669,600원 
837,000원
가격할인
11.01 ~ 12.13
제조사 페이지로 바로가기
His60 Ni Superflow Cartridges User Manual (PT5143-1)_031716.pdf
Clontech
635678
His60 Ni Superflow Cartridge Purification Kit
관련학술 관심상품등록 구매하기
5 ml×5
1,155,200원 
1,444,000원
가격할인
11.01 ~ 12.13
제조사 페이지로 바로가기
Clontech
635657
His60 Ni Gravity Columns
관련학술 관심상품등록 구매하기
1 ml×5
148,000원 
185,000원
가격할인
11.01 ~ 12.13
제조사 페이지로 바로가기
His60 Ni Superflow Resin and Gravity Columns User Manual (PT5017-1)_031816.pdf
Clontech
635658
His60 Ni Gravity Column Purification Kit
관련학술 관심상품등록 구매하기
1 Kit
525,600원 
657,000원
가격할인
11.01 ~ 12.13
제조사 페이지로 바로가기
His60 Ni Superflow Resin and Gravity Columns User Manual (PT5017-1)_031816.pdf
Clontech
635665
His60 Ni Buffer Set
관련학술 관심상품등록 구매하기
20 회
378,400원 
473,000원
가격할인
11.01 ~ 12.13
제조사 페이지로 바로가기
His60 Ni Superflow Resin and Gravity Columns User Manual (PT5017-1)_031816.pdf

His60 Ni Superflow Cartridges using FPLC
Up to 60 mg/ml of his-tagged protein & great purity
For purification under native or denaturing conditions with low nickel ion leakage
Fast and reproducible processing on any LC system, including AKTA
His60 Ni Superflow Cartridges - Overview
His 60 Ni Superflow, the highest capacity resin to date that provides great purity, is now available in prefilled 1 ml and 5 ml cartridges for his-tagged protein purification on any LC system, such as AKTA or FPLC, or for manual purification using a syringe. The resin enables fast, easy, and reproducible chromatographic separations and can be regenerated for multiple uses. For additional information, please refer to the User Manual for His60 Nickel His-Tagged Protein Purification Cartridges.

For additional information about the resin, please see the His60 Ni Superflow Product Page
Protein Purification Principle
His60 Ni Protein Purification Resin, which is based on a modified Ni-IDA resin, provides remarkable selectivity and reliability when is it used to purify proteins containing an affinity tag of six or more consecutive histidine residues―such as the 6xHis tag or other his tags. This technology allows one-step purification of almost any his-tagged protein from any expression system under native or denaturing conditions. The His60 Ni Superflow purification resin has low nickel ion leakage and the highest binding capacity to date (outperforming Ni-NTA and other resins).
His-Tagged Protein Purification Procedure
The purification of recombinant his-tagged proteins consists of 4 steps: cell lysis/extraction, binding, washing, and elution. His60 Nickel allows one-step protein purification under either native or denaturing conditions. The resin is compatible with multiple denaturants and detergents (Table I)―or refer to the User Manual for a complete reagent compatibility table for His60 Ni resin. Purified recombinant his-tagged proteins can be eluted by the addition of 300 mM imidazole or by a reduction in pH.
Applications for Eluted His-Tag Proteins
Purified recombinant his-tagged proteins can be used for:
  • Protein labeling
  • Immunization to produce antibodies
  • Animal studies
  • Other applications
FPLC Application Data for His60 Nickel Cartridges
FPLC protein purification
Step gradient elution using His60 Ni Superflow.
6xHN-tagged AcGFP1 was purified using a 1 ml His60 Ni Superflow-packed cartridge connected to an FPLC system at a flow rate of 1 ml/min following the standard protocol described in the Recombinant His-Tagged Protein Purification User Manual. 1 ml fractions were collected and analyzed (Figure 1).
Performance analysis - comparison to Ni-NTA resin
His60 Ni Superflow resin performance was compared to the performance of Ni- NTA Superflow resin. 6xHN-AcGFP1 was purified from equivalent amounts of the same sample, following each manufacturer’s protocol. Significantly higher yields and better purity of recombinant his-tagged protein were obtained using His60 Ni Superflow resin when compared to Ni-NTA resin (Figure 2).
Reproducibility of Results
His60 Ni Superflow Cartridges were tested for cartridge-to-cartridge performance. Figure 3 presents the chromatography data from three runs on three His60 Ni Superflow Cartridges, using the His60 Ni Buffer Set for protein extraction and purification. A high level of reproducibility was observed between runs, with differences of less than 5% in both protein content and fluorescence signal (data not shown).



Figure 1. Step gradient elution* results of recombinant his-tagged protein purified using His60 Ni Superflow Resin.






Keyword :
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His-tag 융합 단백질 정제
> [선택가이드] His-tag 정제제품
> [개요] His-tag purification
> [FAQ] TALON® Resin
차세대 His-tag purification system
> [개요] 차세대 단백질 정제, Capturem™
Spin column타입으로 빠르고 간편한 His-tag 단백질..
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Histidine tag 융합 단백질의 고순도 정제에
> TALON Resin
> TALON Resin(Magnetic Bead)
TALON Resin 충진한 Pre-packed type
> HisTALON Cartridges & Gravity Columns
대장균으로부터 His-tag 융합 단백질 추출에,xTract..
> xTractor Buffer Kit
Histidine tag 융합 단백질의 고수율로 정제
> His60 Ni Superflow Resin & Gravity Columns
His60 Ni resin 충진제 Pre-packed typ
> His60 Ni Superflow Cartridges & Gravity Columns
> His60 Ni Magnetic Beads
4℃에서 활성을 띄는 HN-tag를 갖고 있는 단백질 분..
> HRV 3C Protease
> Poly Histidine tag 항체
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