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Home > ÀüÁ¦Ç°º¸±â > Stem Cell > Áٱ⼼Æ÷ À¯ÀüÀÚÆíÁý > iPSC Single-Cell Cloning DEF-CS¢â Culture Media Kit
hiPS cellÀÇ single cell ¹è¾ç, À¯ÀüÀÚ ÆíÁý¿¡ ÃÖÀûÀÎ ¹è¾ç ½Ã½ºÅÛ

iPSC Single-Cell Cloning DEF-CS¢â Culture Media Kit

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- Áٱ⼼Æ÷ À¯ÀüÀÚÆíÁý µ¿¿µ»óÀ¸·Î º¸±â
  • hES/iPS cellÀÇ single cell cloning ¹× CRISPR/Cas9 application Á¦°ø
  • Single cell cloningÀ» À§ÇÑ 96 well plate ¹è¾ç¿¡ ÃÖÀûÈ­µÈ ±¸¼º
  • All-in-one systemÀ¸·Î °£ÆíÇÏ°Ô »ç¿ë°¡´É

Single cell cloning protocol
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Cellartis¢ç iPSC Single-Cell Cloning DEF-CS¢â Culture Media Kit (Code Y30021)Àº human ES/iPS cellÀÇ monolayer ¹è¾ç, ±ÕÀÏÇÏ°í ±ú²ýÇÑ ¼¼Æ÷ ºÐÈ­°¡ °¡´ÉÇÑ ±âÁ¸ DEF-CS 500 culture system (Code Y30010)ÀÇ ´É·ÂÀ» ±â¹ÝÀ¸·Î, single cell cloning ´É·ÂÀ» Çâ»ó½ÃŲ Á¦Ç°ÀÌ´Ù. º» Á¦Ç°À» »ç¿ëÇÏ¸é °íÈ¿À²ÀÇ single cell cloningÀÌ °¡´ÉÇϸç, DEF-CS 500°ú °°Àº Àå±â°£ ¹ÌºÐÈ­µµ °¡´ÉÇÏ¿© ±ÕÀÏÇÏ°Ô hiPS cell ¹è¾çÀ» ÇÒ ¼ö ÀÖ´Ù.

Cellartis¢ç iPSC CRISPR/Cas9 Gesicle and Single-Cell Cloning System (Code 632642)´Â ¼¼Æ÷¸· À¯·¡ nanovesicleÀÇ ÀÏÁ¾ÀÎ GesicleÀ» »ç¿ëÇÏ¿© Cas9/sgRNA Ribonucleoprotein (RNP)¸¦ ¼¼Æ÷¿¡ µµÀÔÇÏ´Â CRISPR/Cas9 Gesicle producer system (Code 632613)°ú Cellartis¢ç iPSC Single-Cell Cloning DEF-CS¢â Culture Media Kit (Code Y30021)°¡ Æ÷ÇÔµÈ KitÀÌ´Ù GesicleÀ» ÀÌ¿ëÇϸé hard to transfect cell lineÀÎ human iPS ¼¼Æ÷¿¡ ´ëÇؼ­µµ È¿À²ÀûÀÎ Cas9/sgRNA RNPÀÇ µµÀÔÀÌ °¡´ÉÇϸç, off target effect¸¦ ÁÙÀÏ ¼ö ÀÖ´Ù

Cellartis¢ç iPSC rCas9 Electroporation and Single-Cell Cloning System (Code 632643)Àº Guide-it¢â sgRNA In Vitro Transcription Kit (Code 632635) ¿Í electroporationÀ» »ç¿ëÇÑ ¼¼Æ÷³» µµÀÔ¿¡ ÃÖÀûÀÎ Guide-it¢â Recombinant Cas9 (Electroporation-Ready) (Code 632641) ¹× Cellartis¢ç iPSC Single-Cell Cloning DEF-CS¢â Culture Media Kit (Code Y30021)°¡ Æ÷ÇÔµÈ kitÀÌ´Ù. ±âÁ¸ÀÇ plasmid µµÀÔ¿¡ ºñÇØ electroporationÀ» ÀÌ¿ëÇÑ Cas9/sgRNA RNPÀÇ µµÀÔÀ¸·Î off-target effect¸¦ ÁÙÀÏ ¼ö ÀÖÀ¸¸ç, Cas9 ´Ü¹éÁúÀÇ NLS (Nuclear localization sequence)¿Í electroporation¿¡ ÀûÇÕÇÑ buffer¸¦ »ç¿ëÇÏ¿© °íÈ¿À²ÀÇ Cas9/sgRNA RNP ¼¼Æ÷ ³» µµÀÔÀÌ °¡´ÉÇÏ´Ù.
°ü·ÃÁ¦Ç°¸µÅ©
DEF-CS 500 culture system (Code Y30010)

CRISPR/Cas9 Gesicle producer system (Code 632613)

Guide-it¢â sgRNA In Vitro Transcription Kit (Code 632635)

Guide-it¢â Recombinant Cas9 (Electroporation-Ready) (Code 632641)
Àû¿ë
  • Human iPSC Single-Cell Cloning
  • CRISPR/Cas9-mediated gene editing
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Cellartis¢ç iPSC Single-Cell Cloning DEF-CS¢â Culture Media Kit (Code Y30021)

±¸¼º

¿ë·®

Cellartis DEF-CS 500 Basal Medium

500 ml

Cellartis iPSC Single-Cell Cloning DEF-CS COAT-1

800 §¡ x 2

Cellartis iPSC Single-Cell Cloning DEF-CS additives

1 set

DEF-CS GF-1

750 §¡ x 2

DEF-CS GF-2

500 §¡

DEF-CS GF-3

500 §¡


*±âÁ¸ÀÇ DEF-CS 500 culture system (Y30010)°ú ºñ±³ÇÏ¿©, Single Cell cloningÀ» À§ÇØ Á¦Ç° ±¸¼º°ú Á¶¼ºÀÌ ÃÖÀûÈ­

Cellartis iPSC CRISPR/Cas9 Gesicle and Single-Cell Cloning System (Code 632642)
1.Cellartis¢ç iPSC Single-Cell Cloning DEF-CS¢â Culture Media Kit (Code Y30021)
         À§¿Í °°À½

2.Guide-it CRISPR/Cas9 Gesicle Production System (Code 632613)

  a. Guide-it CRISPR/Cas9 Gesicle Packaging Set (Code 632616)

Guide-it CRISPR/Cas9 Gesicle Packaging Mix 1(green cap)
Guide-it CRISPR/Cas9 Gesicle Packaging Mix 2(yellow cap)
A/C Heterodimerizer(500 nM in Ethanol)
Protamine Sulfate(4 mg/ml)

10 vials
10 vials
50 §¡
200§¡

  b. pGuide-it-sgRNA1 Vector(Linear)(7.5 ng/§¡)
  c. Guide-it Ligation Components v2

20 §¡

DNA Ligation Mighty Mix
Guide-it Oligo Annealing Buffer
Guide-it Control Annealed Oligos v2(100 fmol/§¡)
Guide-it Sequencing Primer 1(100 pmol/§¡)
PCR-Grade Water

50 §¡
1.5 ml
10 §¡
10 §¡
1 ml

  d. Stellar Competent Cells (Code 636763)

Stellar Competent Cells(100§¡/tube)
SOC Medium(1 ml/tube)
pUC19 Vector(0.1 ng/§¡)

10 tubes
10 tubes
10 §¡


Cellartis iPSC rCas9 Electroporation and Single-Cell Cloning System (Code 632643)
1. Cellartis¢ç iPSC Single-Cell Cloning DEF-CS¢â Culture Media Kit (Code Y30021)
         À§¿Í °°À½

2.

Guide-it Recombinant Cas9 (Electroporation-Ready) (Code 632641)

Guide-it Recombinant Cas9 (Electroporation-Ready)

100 §¡

3.

Guide-it sgRNA In Vitro Transcription Kit (Code 632635)

  a. Guide-it sgRNA In Vitro Transcription Components v2

Guide-it Scaffold Template (1 ng/§¡)
Guide-it In Vitro Transcription Buffer
RNase Free Water
Guide-it T7 Polymerase Mix (33 U/§¡)
PrimeSTAR Max Premix (2X)
Recombinant DNase I (RNase-Free) (5 U/§¡)

50 §¡
350 §¡
1 ml
150 §¡
625 §¡
100 §¡

  b. Guide-it IVT RNA Clean-Up Kit£¨Code632638)

RNase Free Water
IVT Binding Buffer
IVT RNA Clean-Up Spin Column
IVT Wash Buffer
Collection Tubes (2 ml)

13 ml
3 ml
50ȸ
6 ml x 2
50 ȸ

º¸°ü
Cellartis DEF-CS 500 Basal Medium£º4¡É
Cellartis iPSC Single-Cell Cloning DEF-CS COAT-1£º4¡É
Cellartis iPSC Single-Cell Cloning DEF-CS Additives£º-20¡É
Guide-it CRISPR/Cas9 Gesicle Packaging Set£º-20¡É
Guide-it Ligation Components v2£º-20¡É
pGuide-it-sgRNA1 Vector (Linear) £º-20¡É
Stellar Competent Cells£º-80¡É
Guide-it Recombinant Cas9 (Electroporation-Ready) £º-70¡É (ÁÖÀÇ)
Guide-it sgRNA In Vitro Transcription Components v2£º-20¡É
Guide-it IVT RNA Clean-Up Kit£º½Ç¿Â

(ÁÖÀÇ) »ç¿ë Áß µ¿°á/À¶ÇØ ¹Ýº¹ ½Ã, »ç¿ë¾çÀ» ¼ÒºÐÇÏ¿© ÀúÀåÇÏ´Â °ÍÀ» ±ÇÀåÇÔ

Keyword : single cell cloning, iPS, stem cell, Áٱ⼼Æ÷, Crispr, SCC

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hiPS cellÀÇ single cell ¹è¾ç, À¯ÀüÀÚ ÆíÁý¿¡ ..
iPSC Single-Cell Cloning DEF-CS¢â Culture Media Kit