³ª¸¸ ¾Ë°í ½ÍÀº ÇÊ»ì cloning ±â¹ý,
In-FusionÀ» ¼Ò°³ÇÕ´Ï´Ù.
In-Fusion¢ç Snap Assembly Master Mix (Code 638947)À» È°¿ëÇϸé vector¿Í insertÀÇ »óµ¿¼¿ À¶ÇÕÀ» ÅëÇØ ¼Õ ½±°Ô cloningÀ» ÇÒ ¼ö ÀÖ½À´Ï´Ù. ±âÁ¸ÀÇ Á¦ÇÑÈ¿¼Ò-ligation ¹æ¹ýº¸´Ù ÈξÀ °£ÆíÇÏ°í ºü¸¥ ½ÇÇè µðÀÚÀÎÀÌ °¡´ÉÇÕ´Ï´Ù.
- Subcloning ºÒÇÊ¿ä - Cloning À§Ä¡, insert ¼¿¿¡ °ü°è¾øÀÌ, ¸ñÀû vector¿¡ ¹Ù·Î cloning
- ³ôÀº È¿À² - 0.5~15 kbÀÇ ´Ù¾çÇÑ Å©±âÀÇ fragment¿¡¼ 95% ÀÌ»óÀÇ Á¤È®µµ
- Seamless construction -ºÒÇÊ¿äÇÑ ¿°±â Ãß°¡ ¾øÀÌ cloning ¿Ï·á ´Ù¾çÇÑ application - multiple cloning, mutagenesis µî ´Ù¾çÇÑ ½ÇÇè
(´õ ¸¹Àº Àû¿ë »ç·Ê¸¦ º¸½Ã·Á¸é Ŭ¸¯Çϼ¼¿ä)
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- ÃÖ°íÀÇ colony Çü¼º´É·Â, ½ÇÇè ¼º°ø·ü UP:
In-Fusion¢ç Snap Assembly Master Mix (Code 638947)
- ÆíÀǼºÀÌ ÁÁÀº ½Ç¿Â º¸°ü µ¿°á°ÇÁ¶ premix:
In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix (Code 638954)
- In-Fusion
¢ç °ú ¦²á, ÃÖ°íÀÇ Á¤È®µµ¸¦ Áö´Ñ PCRÈ¿¼Ò:
PrimeSTAR¢ç Max DNA Polymerase (Code R045A)
- In-Fusion
¢ç Cloning¿¡ ÃÖÀûÈµÈ °íÈ¿À² competent cells:
Stellar¢â Competent Cells (Code 636763)
- Touch down PCR ¹× gradient PCR ¼³Á¤ÀÌ °¡´ÉÇÑ °£ÆíÇÏ°í ÄÄÆÑÆ®ÇÑ PCR ±â±â:
Clontech PCR Thermal Cycler GP (Code WN400)
- Plasmid DNA¸¦ ½Å¼ÓÇÏ°Ô Á¤Á¦:
TaKaRa MiniBEST Plasmid Purification Kit (Code 9760A ¿Ü)