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Home > ´ÙÄ«¶ó ½ºÆ©µð¿À > ¼îÄÉÀ̽º > ³ª¸¸ ¾Ë°í ½ÍÀº ÇÊ»ì cloning


³ª¸¸ ¾Ë°í ½ÍÀº ÇÊ»ì cloning ±â¹ý,
In-FusionÀ» ¼Ò°³ÇÕ´Ï´Ù.


In-Fusion¢ç Snap Assembly Master Mix (Code 638947)À» È°¿ëÇϸé vector¿Í insertÀÇ »óµ¿¼­¿­ À¶ÇÕÀ» ÅëÇØ ¼Õ ½±°Ô cloningÀ» ÇÒ ¼ö ÀÖ½À´Ï´Ù. ±âÁ¸ÀÇ Á¦ÇÑÈ¿¼Ò-ligation ¹æ¹ýº¸´Ù ÈξÀ °£ÆíÇÏ°í ºü¸¥ ½ÇÇè µðÀÚÀÎÀÌ °¡´ÉÇÕ´Ï´Ù.
  • Subcloning ºÒÇÊ¿ä - Cloning À§Ä¡, insert ¼­¿­¿¡ °ü°è¾øÀÌ, ¸ñÀû vector¿¡ ¹Ù·Î cloning
  • ³ôÀº È¿À² - 0.5~15 kbÀÇ ´Ù¾çÇÑ Å©±âÀÇ fragment¿¡¼­ 95% ÀÌ»óÀÇ Á¤È®µµ
  • Seamless construction -ºÒÇÊ¿äÇÑ ¿°±â Ãß°¡ ¾øÀÌ cloning ¿Ï·á ´Ù¾çÇÑ application - multiple cloning, mutagenesis µî ´Ù¾çÇÑ ½ÇÇè
    (´õ ¸¹Àº Àû¿ë »ç·Ê¸¦ º¸½Ã·Á¸é Ŭ¸¯Çϼ¼¿ä)

    

*°ü·Ã Á¦Ç°
- ÃÖ°íÀÇ colony Çü¼º´É·Â, ½ÇÇè ¼º°ø·ü UP: In-Fusion¢ç Snap Assembly Master Mix (Code 638947)
- ÆíÀǼºÀÌ ÁÁÀº ½Ç¿Â º¸°ü µ¿°á°ÇÁ¶ premix: In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix (Code 638954)
- In-Fusion¢ç °ú ¦²á, ÃÖ°íÀÇ Á¤È®µµ¸¦ Áö´Ñ PCRÈ¿¼Ò: PrimeSTAR¢ç Max DNA Polymerase (Code R045A)
- In-Fusion¢ç Cloning¿¡ ÃÖÀûÈ­µÈ °íÈ¿À² competent cells: Stellar¢â Competent Cells (Code 636763)
- Touch down PCR ¹× gradient PCR ¼³Á¤ÀÌ °¡´ÉÇÑ °£ÆíÇÏ°í ÄÄÆÑÆ®ÇÑ PCR ±â±â: Clontech PCR Thermal Cycler GP (Code WN400)
- Plasmid DNA¸¦ ½Å¼ÓÇÏ°Ô Á¤Á¦: TaKaRa MiniBEST Plasmid Purification Kit (Code 9760A ¿Ü)