È¿¼Ò Çϳª ¹Ù²åÀ» »ÓÀε¥, colony ¼ö°¡ WOW!
#Blunt_end, #Sticky_end #TA_cloning_ligation, ¸Å²ô·´°Ô À̾îÁÖ°í ½Í´Ù¸é T4 DNA ligase ´ë½Å ¡°Mighty Mix¡±¸¦ ½áº¸ÀÚ!
DNA Ligation Kit £¼Mighty Mix£¾ (Code 6023)´Â
1 solution typeÀÇ premix ½Ã¾àÀ¸·Î, ´Ù¾çÇÑ Á¶°Ç¿¡¼ ¸Å¿ì °£ÆíÇÏ°Ô °íÈ¿À²·Î ligationÀ» ÁøÇàÇÒ ¼ö ÀÖ°Ô ÇÕ´Ï´Ù (ÀÏ¹Ý plasmid vector ligationÀº 5ºÐ¸¸¿¡ ¿Ï·á!).
*Cloning ½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â Cloning Kit - ¼±Åð¡ÀÌµå ¹Ù·Î°¡±â (Ŭ¸¯)
*°ü·Ã Á¦Ç°
ÀϹÝÀûÀÎ cloning¿¡ »ç¿ëÇÏ´Â ligation kit
- Blunt endÀÇ PCR »ê¹°°ú dA°¡ Ãß°¡µÈ PCR »ê¹° ¸ðµÎ ÇÑ ¹øÀÇ ¹ÝÀÀÀ¸·Î ligation:
Mighty Cloning Reagent Set (Blunt End) (Code 6027)
- 10 kb ÀÌ»óÀÇ long insert cloning¿¡ ÃÖÀû:
TaKaRa DNA Ligation Kit LONG (Code 6024)
TA cloning¿¡ »ç¿ëÇÏ´Â ligation kit
- TA cloning ¿ë T-vector¿Í ligation premix:
Mighty TA-cloning Kit (Code 6028)
- Blunt end PCR »ê¹°ÀÇ TA cloning¿¡ ÃÖÀû:
Mighty TA-cloning Reagent Set for PrimeSTAR¢ç (Code 6019)
°°ÀÌ ¾²¸é È¿À² up!
- Large plasmidµµ È¿À²ÀûÀ¸·Î ÇüÁúÀüȯ:
E. coli HST08 Premium Competent Cells(Code 9128) /
E. coli HST08 Premium Electro-Cells (Code 9028)
- Á¤È®µµ¿Í ÁõÆøÈ¿À² ¸ðµÎ ÃÖÀû, Cloning¿ë PCR È¿¼Ò:
PrimeSTAR¢ç GXL DNA Polymerase (Code R050A)
- Plasmid DNA Á¤Á¦:
TaKaRa MiniBEST Plasmid Purification Kit (Code 9760A)
- Colony PCR¿ë dye plus premix:
EmeraldAmp¢ç GT PCR Master Mix (Code RR310A)