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세포 주기 Monitoring Vector（Fucci）

제조사	제품코드	제품명	용량	가격 (부가세별도)	사용자매뉴얼
Clontech	631462	pRetroX-SG2M-Cyan Vector	10 μg	1,140,000원	,
Clontech	631463	pRetroX-G1-Red Vector	10 μg	1,173,000원	, ,
Clontech	631464	pRetroX-SG2Mcyto-Red Vector	10 μg	1,173,000원	, ,
Clontech	631465	pRetroX-SG2M-Red Vector	10 μg	1,173,000원	, ,
Clontech	631466	pTRE-CellCycle Vector	10 μg	1,163,000원	, ,

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Reporter Systems

Cell Cycle Monitoring Vectors (Fucci)

Single cells or complex tissues-no fixation required!

Easily and precisely track cell-cycle progression in live cells
Monitor phase transitions in cultured cells or live tissue
Visualize cell shape during phase transitions
Label whole cells or just nuclei
Monitor stably expressed phase reporters over long time periods

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Clontech’s cell cycle reporter vectors let you monitor cell cycle progression in living cells, in real-time, without fixation.

Fucci Probes

Our cell cycle reporter vectors deliver fluorescent, ubiquitination-based, cell-cycle indicators (Fucci; 1, 2) that allow you to identify cells in various phases of the cell cycle. These Fucci probes contain Cdt1 or Geminin, proteins whose levels fluctuate differentially throughout the cell cycle: Cdt1 levels peak in G1 phase; as cells transition into S phase, Cdt1 levels fall and Geminin levels rise, remaining high until the cells are back in G1. Cells control Cdt1 and Geminin levels post-translationally, using ubiquitination to target the unwanted proteins for proteasomal degradation. To allow Geminin and Cdt1 to be easily visualized, each is expressed with a red or cyan fluorescent tag. These probes allow precise, visual evaluation of the cell cycle phase (Figure 1).

Monitor Cell Cycle and Shape in Real-Time

Our Fucci cell cycle reporters let you label just the nucleus, or both the nucleus and cytoplasm-allowing visualization of cell shape. Although most Fucci probes label only cell nuclei, we offer a truncated version of hGeminin that is able to migrate to the cytoplasm between S and M phases, enabling the morphology of the cell to be visualized (Table I; 3).

A Variety of Fucci Probes and Delivery Options

We offer retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes (see Table I). Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin. Our retroviral vectors let you express Fucci probes in a wide variety of mammalian cells. Once integrated into the host genome, these vectors provide stable, heritable expression of the probes.

The plasmid-based vector, pTRE-CellCycle (Figure 3), allows tightly controlled, tetracycline (Tet)-inducible expression of two Fucci probes from a bidirectional promoter that can be induced using any Tet-On or Tet-Off technology. Expression of these probes causes cell nuclei to turn red during G1 phase and cyan during S, G2, and M phases, allowing complete visual tracking of the cell cycle. For more information on the latest Tet System technologies, visit the Tet-On 3G Product Page.

Use Clontech’s Fucci vectors to monitor cell cycle progression in live cells, as well as cell cycle phase distributions in a cell population. Our Fucci vectors even let you monitor cell shape as the cells transition through the cell cycle.

Figure 2. Retroviral delivery is available for a variety of Fucci probes. Fucci probes containing Geminin-fluorescent protein (fp) fusions are visible in phases S through M, whereas the probe containing Cdt1-fp (expressed by pRetroX-G1-Red) is visible during the G1 phase. pRetroX-SG2Mcyto-Red expresses a truncated version of geminin that allows the cell shape to be visualized from phases S through M.

References

1.Sakaue-Sawano, A. et al. (2008) Cell 132(3):487-98.
2.Newman, R.H. & Zhang, J. (2008) Chem Biol. 15(2):97-98.
3.Sakaue-Sawano, A. et al. (2008) Chem Biol. 15(12):1243-1248.

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