·Î±×ÀÎ
|
ȸ¿ø°¡ÀÔ
|
ID/PW ã±â
|
Á¦Ç°»ó´ã¹®ÀÇ
°í°´Áö¿ø¼¾ÅÍ
¾÷¹«¾È³»¦¢ÇмúÁö¿ø
ÀüȹøÈ£¦¢
02-2081-2510
Email ¦¢
support@takara.co.kr
´ëÀüÁö»ç
¾÷¹«¾È³»¦¢´ëÀü/ÃæÃ»Áö¿ª ÁÖ¹®, ÇмúÁö¿ø
ÀüȹøÈ£¦¢
042-828-6525
Email ¦¢
tkbd@takara.co.kr
¾÷¹«½Ã°£¾È³»
[ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Products
°øÁö»çÇ×
±â¼úÁö¿ø
Á¤º¸ÀÚ·á
樨毢
ȸ»ç¼Ò°³
That's Good Science!
Á¦Ç°¼±Åð¡À̵å
ÀüÁ¦Ç°º¸±â
Custom Service
COVID-19
À̺¥Æ®/Ä·ÆäÀÎ
´ÙÄ«¶ó¼Ò½Ä
´ÙÄ«¶ó°ø½Ã
½ÅÁ¦Ç°Á¤º¸
´ÙÄ«¶ó ¶óÀ̼±½º
±â¼úÁö¿ø¹®ÀÇ
Á¤º¸ÀÚ·á½Åû
±â±âµ¥¸ð½Åû
¼¼¹Ì³ª½Åû
LS&B
ºê·Î¼Å
eNews
CoA & MSDS
¿Â¶óÀÎÅø
樨毢
¿Â¶óÀμ¼¹Ì³ª
ȸ»ç¼Ò°³
´ë¸®Á¡¾È³»
ã¾Æ¿À½Ã´Â±æ
Welcome
About Thats Good Science
Watch the Videos - S3
Watch the Videos - S2
Watch the Videos - S1
Home
>
ÀüÁ¦Ç°º¸±â
>
Gene transfer (virus)
>
Adenovirus
> Packaging Cell Lines
Packaging Cell Lines
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
632271
Adeno-X 293 Cell Line
1 ml
631,000¿ø
,
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
Titration Kit (qPCR)
Extraction Solution
Lentivirus
Lenti-X ¼±Åð¡À̵å
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
EF1 ¥á promoter º¤ÅÍ ..
Tet-on inducible Expre..
Tet One Inducible Expr..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Á¤È®ÇÑ ¿ª°¡ ÃøÁ¤
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
iPS Cell Generation¢â ..
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
RetroNectin¢ç (Recombi..
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
[ÁÖÀÇ]
µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ ÇØµ¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate ¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.
NOTE:
We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates (e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.
³ôÀº ÇüÁúÀüȯ È¿À²
±âÁ¸ÀÇ strainº¸´Ù °ÇÑ ºÎÂø·Â(adherent)
¼ºÀå ¼Óµµ°¡ ´À¸®±â ¶§¹®¿¡ º¸´Ù È¿À²ÀûÀ¸·Î adenovirus Á¦ÀÛ ¹× ÁõÆø °¡´É
Adeno-X 293 Cell LineÀº Adenovirus¸¦ ¸¸µé±â À§ÇÑ human embryonic kidney cell lineÀÌ´Ù. ±âÁ¸ÀÇ HEK293 ¼¼Æ÷º¸´Ù ¼ºÀå ¼Óµµ°¡ ´À¸®°í, plate¿¡ ºÎÂø·Â(adherent)ÀÌ °Çϸç, overconfluence(°úÀ×Áõ½Ä)¿¡ ÀÇÇÑ ¼¼Æ÷ deathÀ» ¸·°í, ³ôÀº ÇüÁúÀüȯ È¿À²À» Ư¡À¸·Î °¡Áö°í ÀÖ´Ù. ÀÌ·¯ÇÑ Æ¯Â¡À¸·Î È¿°úÀûÀ¸·Î adenovirus Á¦ÀÛ ¹× ÁõÆøÀÌ °¡´ÉÇÏ´Ù.
±×¸² 1. Constructing recombinant adenovirus with In-Fusion technology. DNA sequences can be rapidly transferred as PCR products to any pAdenoX vector using the In-Fusion cloning method. In this example, your gene of interest is amplified with 15 bp extensions that are homologous to the ends of the linearized adenoviral vector. The PCR product is then purified and mixed with the linearized adenoviral vector of choice in the In-Fusion reaction. Following the reaction, a portion of the mixture is transformed into E. coli (Stellar Competent Cells) and screened. Once a PCR-positive clone is identified, the recombinant pAdenoX vector is amplified, purified, and subsequently linearized with the restriction enzyme PacI, then transfected into Adeno-X 293 cells for viral rescue and amplification. Adeno-X GoStix can be used to determine the status of adenovirus rescue.
Applications
Adenovirus Á¦ÀÛ ¹× ÁõÆø
Cell lines Ãë±Þ ÁÖÀÇ »çÇ×
> µ¿°á »óÅ·Π¹è¼ÛµÈ ¼¼Æ÷´Â ±ÇÀå ÇÁ·ÎÅäÄÝ¿¡ µû¶ó ´çÀÏ cell seedingÀ» ±ÇÀåÇÕ´Ï´Ù. ÇØ¿Ü¿¡¼ ±¹³»·Î ¹è¼ÛµÇ´Â °úÁ¤À¸·Î ÀÎÇÏ¿© ¼¼Æ÷ ¹è¾çÀÌ Áö¿¬µÉ °æ¿ì cell viability°¡ ±Þ°ÝÈ÷ °¨¼Ò µÉ ¼ö ÀÖ½À´Ï´Ù.
> Collagen, gelatinÀ̳ª poly-LlysineÀ¸·Î ÄÚÆÃµÈ plate »ç¿ëÀ» Àû±Ø ±ÇÀåÇÕ´Ï´Ù.
> ¼¼Æ÷¹è¾ç½Ã ±ÇÀåÇÏ´Â cell seeding density¸¦ ÁؼöÇÏ¿© ÁֽʽÿÀ. ³Ê¹« ³·Àº seeding density´Â ¼¼Æ÷ÀÇ »ýÀå ¼Óµµ°¡ ³Ê¹« ´Ê°Å³ª ½ÉÁö¾î ¸ðµÎ Á×À» ¼ö ÀÖ½À´Ï´Ù.
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
Keyword :
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression System 3
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi type)
Adenovirus Á¤Á¦ (Mega type)
Real Time PCR·Î Genome copy ¼ö¸¦ ½Å¼ÓÇÏ°Ô Ãø..
qPCR titer ÃøÁ¤ Kit
¸é¿ª ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£¿¡ °¨¿° titer ÃøÁ¤
Hexon ±â¹Ý titer ÃøÁ¤
°£ÆíÇÑ ¿ª°¡ ÃæºÐ ¿©ºÎ °ËÃâ ŰƮ
Adeno-X GoStix
CAR Receptor Booster