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Clontech
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632271
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Adeno-X 293 Cell Line
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1 ml
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737,000¿ø
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,
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Product index
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Adeno-Associated Virus (AA..
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Lentivirus
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Retrovirus
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Adenovirus
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Mammalian Cell ¿ë Vector
[ÁÖÀÇ] µ¿°áµÈ ¼¼Æ÷ÁÖÀÇ Çص¿(Thawing) ½Ã, ¼¼Æ÷ »ýÁ¸À² Çâ»óÀ» À§ÇÏ¿© collagen-coated plate ¶Ç´Â flaks¸¦ »ç¿ëÇϽʽÿÀ.
ÃßÈÄ ¼¼Æ÷ ¹è¾ç ½Ã¿¡µµ ¼¼Æ÷ÁÖÀÇ ºÎÂøÀ²(adherence)ÀÌ ÁÁÁö ¾Ê´Ù¸é collagen-coated plate
¶Ç´Â flask¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.
NOTE: We recommend using collagen-coated plates or flasks for efficient culturing of frozen stocks. Vessels coated
with compounds other than collagen may also provide suitable growth substrates (e.g. poly-L-lysine), but only collagen-coated plates
(e.g. BD BioCoat Cellware, Collagen Type I) have been tested at Clontech. Once recovered, the cells may be cultured directly on tissue
culture plastic. However, if adherence is poor, we recommend using only collagen-coated vessels.
- ³ôÀº ÇüÁúÀüȯ È¿À²
- ±âÁ¸ÀÇ strainº¸´Ù °ÇÑ ºÎÂø·Â(adherent)
- ¼ºÀå ¼Óµµ°¡ ´À¸®±â ¶§¹®¿¡ º¸´Ù È¿À²ÀûÀ¸·Î adenovirus Á¦ÀÛ ¹× ÁõÆø °¡´É
Adeno-X 293 Cell LineÀº Adenovirus¸¦ ¸¸µé±â À§ÇÑ human embryonic kidney cell lineÀÌ´Ù. ±âÁ¸ÀÇ HEK293 ¼¼Æ÷º¸´Ù ¼ºÀå ¼Óµµ°¡ ´À¸®°í, plate¿¡ ºÎÂø·Â(adherent)ÀÌ °Çϸç,
overconfluence(°úÀ×Áõ½Ä)¿¡ ÀÇÇÑ ¼¼Æ÷ deathÀ» ¸·°í, ³ôÀº ÇüÁúÀüȯ È¿À²À» Ư¡À¸·Î °¡Áö°í ÀÖ´Ù. ÀÌ·¯ÇÑ Æ¯Â¡À¸·Î È¿°úÀûÀ¸·Î adenovirus Á¦ÀÛ ¹× ÁõÆøÀÌ °¡´ÉÇÏ´Ù.
±×¸² 1. Constructing recombinant adenovirus with In-Fusion technology. DNA sequences can be rapidly transferred as PCR products to any pAdenoX vector using the
In-Fusion cloning method. In this example, your gene of interest is amplified with 15 bp extensions that are homologous to the ends of the linearized adenoviral
vector. The PCR product is then purified and mixed with the linearized adenoviral vector of choice in the In-Fusion reaction. Following the reaction, a portion
of the mixture is transformed into E. coli (Stellar Competent Cells) and screened. Once a PCR-positive clone is identified, the recombinant pAdenoX vector is
amplified, purified, and subsequently linearized with the restriction enzyme PacI, then transfected into Adeno-X 293 cells for viral rescue and amplification.
Adeno-X GoStix can be used to determine the status of adenovirus rescue.
Applications
Cell lines Ãë±Þ ÁÖÀÇ »çÇ×
> µ¿°á »óÅ·Π¹è¼ÛµÈ ¼¼Æ÷´Â ±ÇÀå ÇÁ·ÎÅäÄÝ¿¡ µû¶ó ´çÀÏ cell seedingÀ» ±ÇÀåÇÕ´Ï´Ù. ÇØ¿Ü¿¡¼ ±¹³»·Î ¹è¼ÛµÇ´Â °úÁ¤À¸·Î ÀÎÇÏ¿© ¼¼Æ÷ ¹è¾çÀÌ Áö¿¬µÉ °æ¿ì cell viability°¡
±Þ°ÝÈ÷ °¨¼Ò µÉ ¼ö ÀÖ½À´Ï´Ù.
> Collagen, gelatinÀ̳ª poly-LlysineÀ¸·Î ÄÚÆÃµÈ plate »ç¿ëÀ» Àû±Ø ±ÇÀåÇÕ´Ï´Ù.
> ¼¼Æ÷¹è¾ç½Ã ±ÇÀåÇÏ´Â cell seeding density¸¦ ÁؼöÇÏ¿© ÁֽʽÿÀ. ³Ê¹« ³·Àº seeding density´Â ¼¼Æ÷ÀÇ »ýÀå ¼Óµµ°¡ ³Ê¹« ´Ê°Å³ª ½ÉÁö¾î ¸ðµÎ Á×À» ¼ö ÀÖ½À´Ï´Ù.
Keyword : Adenovirus Àü¿ë,¹ÙÀÌ·¯½º»ý»ê,Packaging,HEK293,293,293 cell