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Gene transfer (virus)
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Adenovirus
> Adeno-X GoStix
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Adeno-X GoStix
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Clontech
632270
Adeno-X GoStix
20 tests
837,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
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RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
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EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
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Adeno-X GoStix
´Â ¼¼Æ÷º¯¼ºÀÇ ¿µÇâÀÌ °üÂûµÇ±â Àü ¾Æµ¥³ë¹ÙÀÌ·¯½ºÀÇ »óŸ¦ ½Å¼ÓÇÏ°í °£ÆíÇÏ°Ô Å×½ºÆ®ÇÒ ¼ö ÀÖ´Â Á¦Ç°À¸·Î ½Ã°£°ú ³ë·ÂÀ» Àý¾àÇÒ ¼ö ÀÖ´Ù. ¼¼Æ÷¹è¾ç »óÃþ¾× 20 ¥ìl¸¦ GoStixÀÇ cassette¿¡ ³Ö°í buffer 4¹æ¿ï¸¸ ¶³¾îÆ®¸° ÈÄ 2~20ºÐ Á¤µµ¸¸ ±â´Ù¸®¸é ¹êµå°¡ ³ªÅ¸³´Ù. ÀÌ Á¦Ç°À» ÀÌ¿ëÇÏ¸é ¾Æµ¥³ë¹ÙÀÌ·¯½ºÀÇ ³óÃà °úÁ¤¿¡¼ °£´ÜÇÑ Å×½ºÆ®¸¸À¸·Îµµ ¹ÙÀÌ·¯½º ¿ª°¡°¡ ÃæºÐÇÑÁö È®ÀÎ ÇÒ ¼ö Àֱ⠶§¹®¿¡ ³óÃàÀÇ ÀûÁ¤ ½ÃÁ¡À» °áÁ¤ÇÒ ¼ö ÀÖ´Ù. ¹êµå°¡ ÁøÇÒ¼ö·Ï ¾Æµ¥³ë¹ÙÀÌ·¯½ºÀÇ ¿ª°¡°¡ ³ôÀ½À» ÀǹÌÇÑ´Ù.
Rapid adenovirus detection during amplification. Panel A.
Adenovirus expressing ZsGreen1 was amplified in HEK293 cells. 48 hr post-infection, and each day thereafter, the supernatant and the cell pellet were harvested and titers were quantified by counting fluorescent cells in an infectious unit (IFU) assay. The increase in virus level in the cell pellets was mirrored by a proportional increase in the amount of virus collected from the culture supernatant. The amount of virus in the supernatant remained < 10% of the total virus output.
Panel B.
Supernatant serves as clear indicator of total yields. In addition to IFU determination, 20 ¥ìl of each sample was applied to an Adeno-X GoStix. The increase in band intensity paralleled the increase in virus production observed by infectious unit titer.
Use Adeno-X GoStix to instantly confirm the presence of adenovirus in cell culture supernatants or lysates.
The test, which detects adenoviral hexon proteins, requires just 20 ¥ìl of adenoviral supernatant to determine if infectious virus was produced posttransfection, or to select the best time to harvest your virus following amplification. The included Hexon Control (bottom) confirms GoStix test function.
Keyword : ¿ª°¡ÃøÁ¤,Titration,Titer,ŸÀÌÅÍ,¿ª°¡,ELISA,¹ÙÀÌ·¯½º¿ª°¡,¹ÙÀÌ·¯½º ¿ª°¡,Virus genome copies,Virus copy,Virus copies,Adenovirus ¿ª°¡
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression System 3
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi type)
Adenovirus Á¤Á¦ (Mega type)
Real Time PCR·Î Genome copy ¼ö¸¦ ½Å¼ÓÇÏ°Ô Ãø..
qPCR titer ÃøÁ¤ Kit
¸é¿ª ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£¿¡ °¨¿° titer ÃøÁ¤
Hexon ±â¹Ý titer ÃøÁ¤
°£ÆíÇÑ ¿ª°¡ ÃæºÐ ¿©ºÎ °ËÃâ Å°Æ®
Adeno-X GoStix
CAR Receptor Booster