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Gene transfer (virus)
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Adenovirus
> Adenovirus Á¤Á¦ (Maxi type)
Adenovirus Á¤Á¦ (Maxi type)
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Clontech
631533
Adeno-X Maxi Purification Kit
6 ȸ
(Package)
2,092,000¿ø
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
Maxi prepÀ» ÅëÇÏ¿© large scale·Î ¹è¾çµÈ ÀçÁ¶ÇÕ adenovirus Á¤Á¦ °¡´É
Maxi kitÀº 5x15 cm plate Á¤Á¦ °¡´É
CsClÀ̳ª ÃÊ°í¼Ó¿ø½ÉºÐ¸®±â°¡ ºÒÇÊ¿äÇÑ chromatograpy Á¤Á¦
ClontechÀÇ chromatograpy ±â¹ÝÀÇ Adeno-X Purification Kit´Â maxi prep(5 x 15 cm plate) ±Ô¸ðÀÇ ¹è¾ç¾×À¸·ÎºÎÅÍ 1.5 ½Ã°£ À̳»¿¡ ÃÖ´ë 10
13
±îÁö Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. Adeno-X Maxi Purification Kit´Â ³ÐÀº ¹ÙÀÌ·¯½º Á¤Á¦ ¿ë·®À» °¡Áö°í ÀÖ¾î¼ ÃÖ´ë 1 x 10
12
adenovirus ÀÔÀÚ±îÁö Á¤Á¦ ÇÒ ¼ö ÀÖ´Ù.
* Mega ±Ô¸ðÀÇ ¹è¾ç¾× (25 x 15 cm plate)À¸·ÎºÎÅÍ Adenovirus¸¦ Á¤Á¦ÇÏ´Â °æ¿ì
Adeno-X Mega Purification Kit (Code 631032)
Á¦Ç°À» ÃßõÇÕ´Ï´Ù.
°£´ÜÇÑ Á¤Á¦ °úÁ¤
Á¶ÀÛ °úÁ¤¿¡´Â ÃÊ°í¼Ó¿ø½ÉºÐ¸® ´Ü°è°¡ Æ÷ÇԵǾî ÀÖÁö ¾ÊÀº ´ë½Å adenovirus ÀÔÀÚ¸¸À» ¼±ÅÃÀûÀ¸·Î °áÇÕ½ÃÅ°´Â ¸Å¿ì µ¶Æ¯ÇÑ ¸·À» »ç¿ëÇÏ´Â chromatography Á¤Á¦ ¹æ¹ýÀ¸·Î adenovirus¸¦ Á¤Á¦ÇÑ´Ù. ¸·¿¡ °áÇÕµÈ ¹ÙÀÌ·¯½º ÀÔÀÚ´Â ÃÖÃÊÀÇ ¼¼Ã´ °úÁ¤À» °ÅÄ¡¸é¼ Á¤Á¦µÈ ¹ÙÀÌ·¯½º¸¸ ¹èÃâµÇµµ·Ï ÇÑ´Ù (Figure 1). °¢ Á¦Ç°¿¡´Â Á¤Á¦¿¡ ÇÊ¿äÇÑ buffer¿Í ±× ¿Ü ¹°Ç°ÀÌ ¸ðµÎ Æ÷ÇԵǾî ÀÖ´Ù.
°íÇ°ÁúÀÇ ¹ÙÀÌ·¯½º¸¦ ³ôÀº ¿ª°¡·Î »ý»ê
ClontechÀÇ Adeno-X Purification Kit¸¦ »ç¿ëÇÏ¿© CsCl ¹Ðµµ ±¸¹è ¿ø½ÉºÐ¸®¹ý (CsCl density gradient centrifugation)º¸´Ù ¸Å¿ì ³ôÀº ¿ª°¡¿Í °í¼øµµÀÇ ¹ÙÀÌ·¯½º¸¦ ¾òÀ» ¼ö ÀÖ´Ù. CsCl¹ý°ú ´Þ¸® ClontechÀÇ ½ÇÇè¹ýÀº ¾ÈÁ¤ÀûÀ̸ç, ¸¹Àº ±â¼úÀÌ ¿äÇÏÁö ¾ÊÀ» »Ó¸¸ ¾Æ´Ï¶ó °í°¡ÀÇ Àåºñµµ ÇÊ¿äÇÏÁö ¾Ê´Ù. ¿ÀÁ÷ °£´ÜÇÑ Á¶ÀÛÀ¸·Î ³ôÀº ¼öµæÀ²ÀÇ Á¤Á¦µÈ adenovirus¸¦ ¾ÈÁ¤ÀûÀ¸·Î »ý»êÇÒ ¼ö ÀÖ´Ù.
Figure 1. Overview of the Adeno-X Maxi Purification protocol.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Adenovirus Purification Kit (2007) Clontechniques XXII(3):6-7.
Keyword : ¹ÙÀÌ·¯½ºÁ¤Á¦,¹ÙÀÌ·¯½º Á¤Á¦,Á¤Á¦,Adenovirus Á¤Á¦,Virus Á¤Á¦,Virus purification
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression System 3
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi type)
Adenovirus Á¤Á¦ (Mega type)
Real Time PCR·Î Genome copy ¼ö¸¦ ½Å¼ÓÇÏ°Ô Ãø..
qPCR titer ÃøÁ¤ Kit
¸é¿ª ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£¿¡ °¨¿° titer ÃøÁ¤
Hexon ±â¹Ý titer ÃøÁ¤
°£ÆíÇÑ ¿ª°¡ ÃæºÐ ¿©ºÎ °ËÃâ Å°Æ®
Adeno-X GoStix
CAR Receptor Booster