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> CycleavePCR Mycoplasma Detection Kit
Real Time PCR·Î Mycoplasma ½Å¼Ó °ËÃâ
CycleavePCR Mycoplasma Detection Kit
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CycleavePCR Mycoplasma Detection Kit
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M. arginini, M. hominis, M. hyorhinis, M. orale, M. salivarium, M. fermentas, M. bovis, M. arthritidis, M. pirum, M. pneumoniae
) ¹× Acholeplasma ¼Ó ÁßÀÇ 1Á¾(A. laidlawii)¿¡ ´ëÇØ ¸Å¿ì ¹Î°¨ÇÏ°Ô °ËÃâÇÒ ¼ö ÀÖÀ½À» È®ÀÎÇÏ¿´´Ù.
º» kitÀÇ ÁõÆø ¹ÝÀÀ¿¡´Â Hot Start PCR¿ë È¿¼ÒÀÎ TaKaRa Ex Taq HS¸¦ »ç¿ëÇÏ°í ÀÖ¾î ¹ÝÀÀ¾× Á¶Á¦ ȤÀº ¹ÝÀÀ(º»°ÝÀûÀÎ cycle ¹ÝÀÀ)Àü¿¡ miss-primingÀ̳ª primer dimer¿¡¼ À¯·¡ÇÏ´Â ºñƯÀÌÀû ÁõÆøÀ» ¸·À» ¼ö ÀÖ´Ù.
ÁõÆø »ê¹°ÀÇ °ËÃâ¿¡´Â cycling probe¹ýÀ» »ç¿ëÇÏ°í ÀÖ´Ù. ÀÌ ¹æ¹ýÀº RNA¿Í DNA·Î ±¸¼ºµÈ Å°¸Þ¶ó probe(chimera probe)¿Í RNase H¿ÍÀÇ Á¶ÇÕ¿¡ ÀÇÇÑ °í°¨µµ °ËÃâ ¹æ¹ýÀ¸·Î, ¸Å¿ì ƯÀ̼ºÀÌ ³ôÀº °ËÃâÀÌ °¡´ÉÇÏ´Ù. Probe´Â ÇÑÂÊ ¸»´ÜÀº Çü±¤ ¹°Áú·Î, ´Ù¸¥ ¸»´ÜÀº ±× Çü±¤ ¹°ÁúÀÌ ¹ßÇÏ´Â Çü±¤À» ¼Ò±¤ÇÏ´Â ¹°Áú(quencher)·Î Ç¥ÁöµÇ¾î ÀÖ´Ù. ÀÌ cycling probe´Â intactÇÑ »óÅ¿¡¼´Â quenching¿¡ ÀÇÇØ Çü±¤À» ¹ßÇÒ ¼ö ¾øÁö¸¸, »óº¸ÀûÀÎ ÁõÆø »ê¹°°ú ÇÏÀ̺긮µå¸¦ Çü¼ºÇÑ ÈÄ¿¡ RNase H¿¡ ÀÇÇØ RNA ºÎºÐ¿¡¼ Àý´ÜµÇ¸é, °ÇÑ Çü±¤À» ¹ßÇÏ°Ô µÈ´Ù. ÀÌ·¯ÇÑ ¹æ¹ýÀ¸·Î Çü±¤ °µµ¸¦ ÃøÁ¤ÇÏ¿© ÁõÆø »ê¹°·®À» ¸ð´ÏÅÍ ÇÒ ¼ö ÀÖ´Ù. º» kitÀÇ probe´Â Mycoplasma¿¡ Ư¡ÀûÀÎ ¿°±â¿¡ »óº¸ÀûÀ¸·Î °áÇÕÇÏ´Â ¿°±â ºÎºÐÀ» RNA·Î ÇÏ°í Àֱ⠶§¹®¿¡, Mycoplasma¸¸À» ƯÀÌÀûÀ¸·Î °í°¨µµ¿¡ °ËÃâÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù.
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Applied Biosystems 7500 Fast Real-Time PCR System(Life Technologies)
* 200 ¥ìl, 20 ¥ìl, 10 ¥ìl °¢ micropipette, tips
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