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MycoAlert PLUS mycoplasma detection kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Lonza
LT07-701
MycoAlert PLUS Mycoplasma detection kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 tests
°¡°Ý¹®ÀÇ   MycoAlert_PLUS_Mycoplasma_Detection_Kit.pdf
Lonza
LT07-703
MycoAlert PLUS Mycoplasma detection kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
30 tests
°¡°Ý¹®ÀÇ  
Lonza
LT07-705
MycoAlert PLUS Mycoplasma detection kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 tests
°¡°Ý¹®ÀÇ  
Lonza
LT07-710
MycoAlert PLUS Mycoplasma detection kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 tests
°¡°Ý¹®ÀÇ  
Lonza
LT07-518
MycoAlert Assay Control Set
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 tests
°¡°Ý¹®ÀÇ   MycoAlert_Assay_Control.pdf

- MycoAlert PLUS Mycoplasma Detection KitÀº ±âÁ¸ÀÇ MycoAlert KitÀÇ ´ÙÀ½ ¹öÀü (next generation)À¸·Î º¸´Ù ³ôÀº light out putÀ» Á¦°øÇÑ´Ù.
- MycoAlert¢â PLUS Mycoplasma Detection Kit¿¡ ÃÖÀûÈ­µÈ Lucetta¢â Luminometer Ãâ½Ã!
Á¦Ç°¼³¸í
MycoAlert PLUS Assay´Â ¼¼Æ÷¹è¾ç¿¡¼­ °¨¿°µÇ´Â 6Á¾ÀÇ ÁÖ¿ä mycoplasma¿Í ´Ù¾çÇÑ 180Á¾ÀÇ mycoplasmaÁ¾À» °ËÃâÇÒ ¼ö ÀÖ´Ù(not present in eukaryotic cells). MycoAlert PLUS Assay´Â mycoplasma°¡ °¡Áö´Â È¿¼Ò È°¼ºÀ» ÀÌ¿ëÇÑ´Ù. »ùÇÃ(cell supernatant or fresh media or supplements)ÁßÀÇ mycoplasma¸¦ ¿ëÇØÇÏ¿©, mycoplasmaÀÇ È¿¼Ò¸¦ MycoAlert Substrate¿Í ¹ÝÀÀ½ÃÅ°¸é ADP°¡ ATP·Î º¯È¯µÈ´Ù. MycoAlert Substrate¸¦ ÷°¡ Àü (A), ÈÄ (B)ÀÇ »ùÇÃÀÇ ATP levelÀ» ÃøÁ¤ÇÏ¿©, Reading B/ Reading AÀÇ ºñÀ²À» ±¸ÇÏ¿© »ùÇà ÁßÀÇ Mycoplasma ÀÇ À¯¹«¸¦ ÆÇÁ¤ÇÒ ¼ö ÀÖ´Ù. ´ëºÎºÐÀÇ Mycoplasma, Acholeplasma ÀÇ °ËÃâÀÌ °¡´ÉÇÏ´Ù.

MycoAlert PLUS Assay¸¦ ÀÌ¿ëÇÑ mycoplasma °ËÃâ ÇÁ·ÎÅäÄÝ
Cell culture supernatant (100 ul)
¡é
Add MycoAlert¢â PLUS Reagent (Wait 5 min)
¡é
Measure luminescence (Reading A)
¡é
Add MycoAlert¢â PLUS Substrate (Incubate 10 min)
¡é
Measure luminescence (Reading B)
¡é
Ratio B/A > 1 equals mycoplasma contamination
Ư¡
- ¼¼Æ÷¹è¾ç¾×À¸·ÎºÎÅÍ °ËÃâ±îÁö 20ºÐ Á¤µµ ¼Ò¿ä (±âÁ¸ÀÇ ´Ù¸¥ ¹æ¹ýÀº ¼ö ½Ã°£¿¡¼­ ¼ö ÁÖÀϱîÁö ¼Ò¿ä)
- ¼¼Æ÷¹è¾ç¾× 100 ul¿¡ µÎ °³ÀÇ ½Ã¾àÀ» È¥ÇÕÇÑ ÈÄ, Luminometer·Î µÎ ¹ø¸¸ ÃøÁ¤ÇÏ¸é µÊ.
- »õÆ÷¹è¾ç½Ã º¸Åë ¿À¿°µÇ´Â ¸ðµç Á¾ÀÇ Mycoplasma¿Í Acholeplasma °ËÃâ °¡´É.
  (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma; except Ureaplasma)
MycoAlert™ Assay Control Set (available separately)
MycoAlert Assay Control Set´Â µ¿°á°ÇÁ¶µÈ positive control (1§¢)°ú assay buffer·Î ±¸¼ºµÇ¾î ÀÖ´Ù. º» Å°Æ®¸¦ ÀÌ¿ëÇÏ¿© ¸ðµç ½ÇÇè°úÁ¤°ú ¸ðµç assay ½Ã¾àÀÌ ¿Ã¹Ù¸£°Ô »ç¿ëµÇ¾ú´ÂÁö¸¦ È®ÀÎÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ 1) Assay buffer´Â À½¼º ´ëÁ¶±ºÀ¸·Î »ç¿ëÀÌ °¡´ÉÇÏ´Ù. ¾ç¼º ´ëÁ¶±º¿¡´Â ½ÇÁ¦ mycoplasma´Â Æ÷ÇԵǾî ÀÖÁö ¾Ê´Ù.

Keyword : mycozap, mycoZap