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Home > ÀüÁ¦Ç°º¸±â > Ç×ü ¡¤ ELISA ¡¤ Signal > Apoptosis > In situ Apoptosis Detection Kit
TUNEL¹ýÀ» ÀÌ¿ëÇÑ Apoptosis °ËÃâ

In situ Apoptosis Detection Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
MK500
in situ Apoptosis Detection Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ȸ
322,400¿ø 
403,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
MK500_e.v2111Da.pdf
Takara
MK501
Labeling Safe Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 ¥ìl¡¿10
660,800¿ø 
826,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
Takara
MK502
TdT Enzyme
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ¥ìl¡¿10
304,800¿ø 
381,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
Takara
MK503
Anti-FITC HRP Conjugate
°ü·ÃÇмú ±¸¸ÅÇϱâ
1.5 ml¡¿5
279,200¿ø 
349,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
Takara
MK504
Control Slides
°ü·ÃÇмú ±¸¸ÅÇϱâ
2 Slides¡¿5
133,600¿ø 
167,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
Takara
MK505
Permeabilisation Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
1.0 ml¡¿10
112,800¿ø 
141,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31

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1. Labeling Safe Buffer 500 §¡¡¿2 vials
2. TdT Enzyme 50 §¡¡¿2 vials
3. Anti-FITC HRP Conjugate
4. Control Slides 2 slides
5. Permeabilisation Buffer 1.0 §¢¡¿2 vials

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3: 4¡É (¼ö¼Û ½Ã´Â -20¡É. Çѹø À¶ÇØÇÑ ÈÄ¿¡´Â 4¡É¿¡ º¸Á¸)
4: ½Ç¿Â (¼ö¼Û ½Ã´Â -20¡É)
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º» Å°Æ®´Â apoptosis¸¦ ÀÏÀ¸Å°´Â ¼¼Æ÷ÀÇ Æ¯Â¡ Áß ÇϳªÀÎ ´ÜÆíÈ­µÈ chromatin DNA¸¦ ¸»´ÜÇ¥½Ä¹ý¿¡ ÀÇÇØ Á¶Á÷È­ÇÐÀûÀ¸·Î °ËÃâÇÏ´Â Å°Æ®ÀÌ´Ù. In situ¿¡¼­ Ç¥½ÄÇÙ»êÀÇ ÇÔÀÔ (incorporation)À¸·Î apoptosisÀÇ Á¶Á÷ÇÐÀû ºÐÆ÷³ª °¢°¢ÀÇ ¼¼Æ÷¸¦ È¿À²ÀûÀ¸·Î °ËÃâÇÏ´Â TUNEL (TdT-mediated dUTP-biotin nick end labeling)¹ýÀ» ÀÌ¿ëÇÏ¿© Á¶Á÷´ÜÆí, °íÁ¤¼¼Æ÷ µîÀÇ ¾î¶² ½Ã·á¿¡µµ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç Çü±¤Çö¹Ì°æ, ±¤ÇÐÇö¹Ì°æ, flow cytometry·Îµµ °üÂûÀÌ °¡´ÉÇÏ´Ù.
º» Å°Æ®¿¡´Â ¸é¿ª¹ÝÀÀ¿ë ±âÁúÀ̳ª ¿°»ö¿ë ½Ã¾àÀº Æ÷ÇԵǾî ÀÖÁö ¾Ê´Ù. Control Slide´Â ·¡Æ®(rat) À¯¼±Á¶Á÷ÀÇ ÆĶóÇÉ (paraffin) Æ÷¸Å´ÜÆíÀ̸ç, Positive Control Slide·Î »ç¿ëÇÒ ¶§´Â ÆĶóÇÉÀ» Á¦°ÅÇÑ ÈÄ¿¡ ÷ºÎÇÑ »ç¿ë¼³¸í¼­ÀÇ ¡¸Á¶ÀÛ¼ø¼­¡¹ (ÆĶóÇÉ Æ÷¸Å´ÜÆíÀÇ °æ¿ì)¿¡ µû¶ó »ç¿ëÇÑ´Ù.
Å»ÆĶóÇÉ°ú Proteinase K¸¦ ó¸®ÇÑ ÈÄ¿¡´Â °üÂû¹æ¹ý¿¡ µû¶ó ÀûÀýÇÑ ÇÁ·ÎÅäÄÝ·Î Á¶ÀÛÇÑ´Ù.
Å°Æ®ÀÇ component´Â ´ÜÇ° (Bulk)À¸·Îµµ ÆǸÅÇÑ´Ù.
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TUNEL¹ýÀº apoptosis¸¦ ÀÏÀ¸Å² ¼¼Æ÷¿¡¼­ ´ÜÆíÈ­µÈ DNAÀÇ À¯¸® 3¡¯-OH ¸»´ÜÀ» terminal transferase (TdT)·Î fluorescein-dUTP¸¦ °íÈ¿À²ÀûÀ̸鼭µµ ƯÀÌÀûÀ¸·Î Ç¥½ÄÇÑ ÈÄ Çü±¤Çö¹Ì°æÀ̳ª flow cytometry·Î °ËÃâÇÏ´Â ¹æ¹ýÀÌ´Ù. ÇÔÀÔµÈ fluoresceinÀº peroxidase Ç¥½Ä anti-fluorescein Ç×ü¸¦ ÀÌ¿ëÇÑ ¹ß»ö¹ÝÀÀÀ» ÅëÇÏ¿© ±¤ÇÐÇö¹Ì°æÀ¸·Îµµ °üÂûÇÒ ¼ö ÀÖ´Ù.

Keyword :

Apoptosis
>
Apoptosis·Î ÀÎÇÑ ´ÜÆíÈ­ DNA °ËÃâ
ApopLadder Ex¢â
>
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ApoPrimer Set (Bcl-2 family)
>
TUNEL¹ýÀ» ÀÌ¿ëÇÑ Apoptosis °ËÃâ
In situ Apoptosis Detection Kit