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 Á¦¸ñ : [°øÁö] ½ÅÁ¦Ç° Ãâ½Ã, mouse TCR ´Ù¾ç¼ºÀ» ºÐ¼®ÇÏ´Â NGS kit
ÀÛ¼ºÀÏ : 2023.12.04
 ÀÛ¼ºÀÚ : ´ÙÄ«¶óÄÚ¸®¾Æ

SMART-Seq NGS kit ½ÅÁ¦Ç° Ãâ½Ã, UMIs Àû¿ëÇÑ mouse TCR Profiling kit

 

¾È³çÇϼ¼¿ä´ÙÄ«¶óÄÚ¸®¾ÆÀÔ´Ï´Ù.

SMARTer Mouse TCR a/b Profiling Kit (634402 ¿Ü) Á¦Ç°ÀÇ ¾÷±×·¹À̵å Á¦Ç°ÀÌ Ãâ½ÃµÇ¾ú½À´Ï´Ù. 

º» Á¦Ç°Àº °¨¿°¼º Áúȯ, ¹é½Å µîÀÇ ¿¬±¸¸¦ À§ÇØ À¯¿ëÇÑ Á¦Ç°À̸ç, T-cell receptor (TCR) repertoire NGS ºÐ¼®À» À§ÇÏ¿© Illumina platform NGS Library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÔ´Ï´Ù.  

 

New!! SMART-Seq¢ç Mouse TCR (with UMIs) Ãâ½Ã 

  • ³ÐÀº ¹üÀ§ÀÇ »ùÇà input Àû¿ë
  • ³ôÀº °¨µµ, ½ÉÇÃÇÑ ¿öÅ©Ç÷ο젠
  • Sequencing flexibility: TCR ¥á ¿Í TCR ¥â subunitÀÇ full-length V(D)J ¶Ç´Â CDR3¸¸ Sequencing °¡´É
  • UMIs Àû¿ëÀ¸·Î ±Ø¼Ò·® »ùÇÿ¡¼­µµ PCR duplicates, sequencing error ±³Á¤ °¡´É
  • Unique Dual Index Kit ¿Í ȣȯµÇ¾î ÃÖ´ë 384°³ »ùÇÃÀ» µ¿½Ã¿¡ ½ÃÄö½Ì °¡´É (*UDI º°µµ±¸¸Å)
  • ¹«·á ºÐ¼®Åø Áö¿ø

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SMART-Seq¢ç Mouse TCR (with UMIs)

(mTCRv2) New!!

SMARTer¢ç Mouse TCR a/b Profiling Kit 

(mTCRv1)

Code

634815, 634816, 634814

634402, 634403, 634404

Input

 

  • 1 ng - 1 §¶ RNA (spleen, T cells)
  • 10 ng - 1 §¶ RNA (whole blood, bone marrow, thymus)
  • 100 - 10,000 purified T cells

 

 

  • 10 ng - 500 ng RNA (spleen, thymus, PBMC)
  • 1,000 - 10,000 purified T cells

 

UMIs

Àû¿ë

¹ÌÀû¿ë

UDIs

ȣȯµÊ (º°µµ±¸¸Å)

ȣȯµÇÁö ¾ÊÀ½

Sequencing
À¯¿¬¼º

Full-length V(D)J ¶Ç´Â CDR3¸¸ Sequencing °¡´É

2 x 300 bpÀ¸·Î¸¸ Sequencing °¡´É

Bioinformatics tool Á¦°ø

´ç»ç ¹«·á tool Á¦°ø
(Cogent IP v1.6
·Î µ¥ÀÌÅÍ ºÐ¼® °¡´É)

 

*Unique Dual Index Kit (º°¸Å)·Î ÃÖ´ë 384°³ÀÇ »ùÇÃÀ» multiplexing ºÐ¼® °¡´É 

 

RNA-Seq Á¦Ç°ÀÌ ¼øÂ÷ÀûÀ¸·Î ¸®´º¾óµÇ°í ÀÖ½À´Ï´Ù. ÀÚ¼¼ÇÑ »çÇ×Àº Çϱ⠾ȳ» ÆäÀÌÁö¸¦ Âü°íÇØÁÖ¼¼¿ä. 

>> RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸°Ô ¹Ù²ò´Ï´Ù 


Á¦Ç° °ü·ÃµÈ ¹®ÀÇ»çÇ×Àº °í°´Áö¿ø (02-2081-2510, support@takara.co.kr) ·Î ¿¬¶ô ¹Ù¶ø´Ï´Ù. 

 

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