구성품

용량

Faustovirus Capping Enzyme (S17) (25 U/μl)

500 U

10×Capping Buffer 2

100 ㎕

S-adenosylmethionine (SAM) (32 mM)

100 ㎕

GTP (10 mM)

50 ㎕

1 x

Capping Buffer 2

25 ㎕

GTP

20 μCi/㎖

[3H]SAM

2.5 ㎍/50 ㎕

100 nt RNA

RNA transcript (50 ㎍)^*1

38 ㎕

10x Capping Buffer 2

5 ㎕

GTP (10 mM)

2.5 ㎕

SAM (2 mM, dilute 32 mM stock to 2 mM)^*2

2.5 ㎕

Faustovirus Capping Enzyme (S17)^*3

2 ㎕

Total

50 ㎕ ^*4

RNA transcript (50 ㎍)^*1

34 ㎕

10x Capping Buffer 2

5 ㎕

GTP (10 mM)

2.5 ㎕

SAM (4 mM, dilute 32 mM stock to 4 mM)^*2

2.5 ㎕

Faustovirus Capping Enzyme (S17)^*3

2 ㎕

mRNA Cap 2’-O-Methyltransferase (50 U/㎕)^*3

4 ㎕

Total

50 ㎕ ^*4