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Home > ÀüÁ¦Ç°º¸±â > Àü±â¿µµ¿ °ü·Ã > ¿°»ö½Ã¾à > SYBR Green I/II Nucleic Acid Stain
EtBr º¸´Ù °í°¨µµ·Î ÇÙ»ê °ËÃâ

SYBR Green I/II Nucleic Acid Stain

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Lonza
50512
SYBR¢ç Green I Nucleic Acid Stain
°ü·ÃÇмú ±¸¸ÅÇϱâ
2 ¡¿ 500 §¡
°¡°Ý¹®ÀÇ   50512-1.pdf
Lonza
50513
SYBR¢ç Green I Nucleic Acid Stain
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ¡¿ 50 §¡
°¡°Ý¹®ÀÇ  
Lonza
50523
SYBR¢ç Green II Nucleic Acid Gel Stain
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ¡¿ 50 §¡
°¡°Ý¹®ÀÇ   29594.pdf

SYBR¢ç Green Nucleic Acid Gel Stain series
  • SYBR¢ç Green I Nucleic Acid Stain
    DNA¿Í RNA¸¦ °ËÃâÇÒ ¼ö ÀÖ´Â Çü±¤ ¿°»ö ½Ã¾àÀ¸·Î EtBr°ú °°Àº ÀϹÝÀûÀÎ ¿°»ö½Ã¾à¿¡ ºñÇØ °¨µµ´Â ³ô°í, µ¹¿¬º¯À̸¦ À¯¹ßÇÒ °¡´É¼ºÀº ³·Àº ÀåÁ¡ÀÌ ÀÖ´Ù. ¶ÇÇÑ ³ôÀº signal-to-noise ratio¿Í ³·Àº background·Î agarose gel°ú polyacrylamide gel¿¡¼­ 60 pg dsDNA ¶Ç´Â 1 ngÀÇ oligonucleotide¸¦ °ËÃâÇÒ ¼ö ÀÖ´Ù.
    PCR ÁõÆø»ê¹° È®ÀÎ, apoptosis ¿¬±¸, heteroduplex ºÐ¼® µî¿¡ È°¿ëÇÒ ¼ö ÀÖÀ¸¸ç, gelÀ» ¸¸µé±â Àü agarose solution¿¡ ÷°¡Çϰųª Àü±â¿µµ¿ÀÌ ³¡³­ ÈÄ gelÀ» ¿°»öÇÏ´Â ¹æ¹ýÀ¸·Î »ç¿ëÇÑ´Ù.
  • SYBR¢ç Green II Nucleic Acid Gel Stain
    ssDNA, RNA¸¦ °ËÃâÇϱâ À§ÇÑ Çü±¤ ¿°»ö ½Ã¾àÀ¸·Î agarose gel°ú polyacrylamide gel¿¡¼­ 100 pg ssDNA ¶Ç´Â 2 ng RNA¸¦ °ËÃâÇÒ ¼ö ÀÖ¾î RNA gel Àü±â¿µµ¿ ¹× SSCP ºÐ¼®¿¡ ÃÖÀûÀÌ´Ù.
    SYBR¢ç Green I°ú SYBR¢ç Green II´Â ¸ðµÎ DMSO¿¡ 10,000x ³óÃàµÈ ÇüÅ·ΠÁ¦°øµÈ´Ù.
  • SYBR¢ç Green Gel Stain II Photographic Filter
    Èæ¹é Æú¶ó·ÎÀ̵å Ä«¸Þ¶ó »ç¿ë½Ã ÇÔ²² »ç¿ëÇÏ´Â Àü¿ë »ç°¢ ÇÊÅÍ(75 mm ¡¿ 75 mm) ·Î ´ëºÎºÐÀÇ Æú¶ó·ÎÀÌµå ½Ã½ºÅÛ¿¡ Àß Àû¿ëÇÒ ¼ö ÀÖ´Ù.
    * º» ÇÊÅÍ´Â Ä«¸Þ¶óÀÇ ±âÁ¾¿¡ µû¶ó »ç¿ëÇÒ ¼ö ¾ø´Â °æ¿ì°¡ ÀÖÀ¸¹Ç·Î Ä«¸Þ¶ó ±¸ÀÔó¿¡ ¹®ÀÇÇÏ¿© Áֽñ⠹ٶø´Ï´Ù.
Ư¡
  • High sensitivity
  • Low signal to noise ratio
Àü±â¿µµ¿ ¿¹
1. DNA Àü±â¿µµ¿: SYBR¢ç Green I Stain°ú EtBrÀÇ °¨µµ ºñ±³

DNA samples (pBR322 Msp I digest) ranging from 1 to 200 ng per lane were separated on a 10 cm x 16 cm x 0.1 cm, 4% vertical MetaPhor Agarose gel prepared in 1X TBE Buffer. The gel war ren for 1 hours at 488V/cm. Following electrophoresis the gel was divided into two, and one half was stained with 1 §¶/§¢ ethidium bromide while the other was stained with SYBR¢ç Green I Stain (1:10,000 dilution of stock). Detection was achieved with standard 300 nm UV transillumination.

2. RNA Àü±â¿µµ¿: SYBR¢ç Green II Stain°ú EtBrÀÇ °¨µµ ºñ±³

Samples of E. coli total RNA were denatured using the following denaturants:
Lane A: Formaldehyde/ Formamide; Lane B: Formamide; Lane C: Glyoxal. Samples were loaded at 2 §¶/lane for the formaldehyde/formamide and formamide only denatured samples, and 4 §¶/lane for the glyoxal denatured samples. Reliant¢â RNA precast Agarose gels were run at 7 V/cm for 40 minutes in 1X MOPS Buffer and post stained with SYBR Green II¢ç gel stain and photo graphed on the Clare Chemical Research, Inc., Dark Reader¢ç Transilluminator.
°ü·ÃÁ¦Ç°
- No.1 agarose: SeaKem¢ç LE Agarose
- »ç¿ëÀÌ °£ÆíÇÑ premade buffer: Lonza AccuGene¢ç Buffer ½Ã¸®Áî
- Gradient PCR ±â±â: TaKaRa PCR Thermal Cycler Touch¢ç
- ³»¿­¼º gel maker set¸¦ Æ÷ÇÔÇÑ µðÁöÅÐ Àü±â¿µµ¿ ÀåÄ¡: Mupid¢ç-One

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