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> SYBR Green I/II Nucleic Acid Stain
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SYBR Green I/II Nucleic Acid Stain
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Lonza
50512
SYBR¢ç Green I Nucleic Acid Stain
2 ¡¿ 500 §¡
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Lonza
50513
SYBR¢ç Green I Nucleic Acid Stain
10 ¡¿ 50 §¡
°¡°Ý¹®ÀÇ
Lonza
50523
SYBR¢ç Green II Nucleic Acid Gel Stain
10 ¡¿ 50 §¡
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Product index
Lonza buffer
AccuGENE PBS buffer
AccuGENE¢â Neutralizat..
ºÐÀÚ·® Marker
Digested Markers ¸ð½Ä..
DNA Marker ºÐÀÚ·® À϶÷..
Ladder Makers ¸ð½Äµµ
0.5-10 kb ssRNA Ladder..
14-30 ssRNA Ladder Mar..
DNA Digested Markers (..
DNA Ladder Markers Dye..
DNA Other Markers (Aga..
FlashGel DNA RNA Marke..
Lonza DNA Markers Ladd..
Lonza SimplyLoad DNA L..
Megabase DNA Ladder
Protein Colored and Un..
Protein Marker
RNA Marker (0.5 to 9 k..
siRNA Ladder Marker
Wide-Range DNA Ladder ..
Agarose
DNA, RNA Àü±â¿µµ¿ ¼±ÅÃ..
[FAQ] Àü±â¿µµ¿ °ü·Ã (A..
SeaKem LE Agarose
Agarose H14¡¸TAKARA¡¹
Agarose L03¡¸TAKARA¡¹
I.D.NA Agarose
IEF : IsoGel Agarose ..
´Ü¹éÁú ºÐ¸®¿ë Agarose
MetaPhor Agarose
NuSieve 3:1 Agarose
NuSieve GTG Agarose
SeaKem Gold Agarose
SeaKem GTG Agarose
SeaPlaque Agarose
SeaPlaque GTG Agarose
SeaPrep Agarose
Precast Agarose Gel£¯¿µµ¿ ..
[°³¿ä] So Easy Product
DNA ºÐ¼®¿ë FlashGel¢â ..
ȸ¼ö¿ë FlashGel¢â Syst..
RNA ºÐ¼®¿ë FlashGel¢â ..
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Minigel - Reliant Gel ..
Reliant¢â RNA Gel Syst..
Latitude¢â Midigel Cha..
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GelStar¢ç Nucleic Acid..
SYBR Green I/II Nuclei..
Protein Gel Stains : S..
SYPRO Ruby Protein Sta..
±âŸ Á¦Ç° (Àü±â¿µµ¿ °ü·Ã)
6 x Loading Buffer
Protein Loading buffer..
EDTA Buffer Powder
GelBond Film and GelBo..
Loading & Sample Buffe..
Àü±â¿µµ¿ Buffer
Molecular Buffer ½Ã¸®..
Tris Buffer Powder
Tris-Acetate-EDTA(TAE)..
Tris-EDTA Buffer (TBE)..
Tris-EDTA Buffer (TE) ..
Àü±â¿µµ¿ ÀåÄ¡ ¡¤ Gel ÃÔ¿µ ..
Àü±â¿µµ¿ FAQ
[°³¿ä] So Easy Product
Mupid Àü±â¿µµ¿ ÀåÄ¡¿Í ..
Mupid-2plus
Mupid-exU
Mupid-One
SYBR
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Green Nucleic Acid Gel Stain series
SYBR
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Green I Nucleic Acid Stain
DNA¿Í RNA¸¦ °ËÃâÇÒ ¼ö ÀÖ´Â Çü±¤ ¿°»ö ½Ã¾àÀ¸·Î EtBr°ú °°Àº ÀϹÝÀûÀÎ ¿°»ö½Ã¾à¿¡ ºñÇØ °¨µµ´Â ³ô°í, µ¹¿¬º¯À̸¦ À¯¹ßÇÒ °¡´É¼ºÀº ³·Àº ÀåÁ¡ÀÌ ÀÖ´Ù. ¶ÇÇÑ ³ôÀº signal-to-noise ratio¿Í ³·Àº background·Î agarose gel°ú polyacrylamide gel¿¡¼ 60 pg dsDNA ¶Ç´Â 1 ngÀÇ oligonucleotide¸¦ °ËÃâÇÒ ¼ö ÀÖ´Ù.
PCR ÁõÆø»ê¹° È®ÀÎ, apoptosis ¿¬±¸, heteroduplex ºÐ¼® µî¿¡ È°¿ëÇÒ ¼ö ÀÖÀ¸¸ç, gelÀ» ¸¸µé±â Àü agarose solution¿¡ ÷°¡Çϰųª Àü±â¿µµ¿ÀÌ ³¡³ ÈÄ gelÀ» ¿°»öÇÏ´Â ¹æ¹ýÀ¸·Î »ç¿ëÇÑ´Ù.
SYBR
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Green II Nucleic Acid Gel Stain
ssDNA, RNA¸¦ °ËÃâÇϱâ À§ÇÑ Çü±¤ ¿°»ö ½Ã¾àÀ¸·Î agarose gel°ú polyacrylamide gel¿¡¼ 100 pg ssDNA ¶Ç´Â 2 ng RNA¸¦ °ËÃâÇÒ ¼ö ÀÖ¾î RNA gel Àü±â¿µµ¿ ¹× SSCP ºÐ¼®¿¡ ÃÖÀûÀÌ´Ù.
SYBR
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Green I°ú SYBR
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Green II´Â ¸ðµÎ DMSO¿¡ 10,000x ³óÃàµÈ ÇüÅ·ΠÁ¦°øµÈ´Ù.
SYBR
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Green Gel Stain II Photographic Filter
Èæ¹é Æú¶ó·ÎÀ̵å Ä«¸Þ¶ó »ç¿ë½Ã ÇÔ²² »ç¿ëÇÏ´Â Àü¿ë »ç°¢ ÇÊÅÍ(75 mm ¡¿ 75 mm) ·Î ´ëºÎºÐÀÇ Æú¶ó·ÎÀÌµå ½Ã½ºÅÛ¿¡ Àß Àû¿ëÇÒ ¼ö ÀÖ´Ù.
* º» ÇÊÅÍ´Â Ä«¸Þ¶óÀÇ ±âÁ¾¿¡ µû¶ó »ç¿ëÇÒ ¼ö ¾ø´Â °æ¿ì°¡ ÀÖÀ¸¹Ç·Î Ä«¸Þ¶ó ±¸ÀÔó¿¡ ¹®ÀÇÇÏ¿© Áֽñ⠹ٶø´Ï´Ù.
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High sensitivity
Low signal to noise ratio
Àü±â¿µµ¿ ¿¹
1. DNA Àü±â¿µµ¿: SYBR
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Green I Stain°ú EtBrÀÇ °¨µµ ºñ±³
DNA samples (pBR322
Msp
I digest) ranging from 1 to 200 ng per lane were separated on a 10 cm x 16 cm x 0.1 cm, 4% vertical MetaPhor Agarose gel prepared in 1X TBE Buffer. The gel war ren for 1 hours at 488V/cm. Following electrophoresis the gel was divided into two, and one half was stained with 1 §¶/§¢ ethidium bromide while the other was stained with SYBR
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Green I Stain (1:10,000 dilution of stock). Detection was achieved with standard 300 nm UV transillumination.
2. RNA Àü±â¿µµ¿: SYBR
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Green II Stain°ú EtBrÀÇ °¨µµ ºñ±³
Samples of
E
.
coli
total RNA were denatured using the following denaturants:
Lane A: Formaldehyde/ Formamide; Lane B: Formamide; Lane C: Glyoxal. Samples were loaded at 2 §¶/lane for the formaldehyde/formamide and formamide only denatured samples, and 4 §¶/lane for the glyoxal denatured samples. Reliant¢â RNA precast Agarose gels were run at 7 V/cm for 40 minutes in 1X MOPS Buffer and post stained with SYBR Green II
¢ç
gel stain and photo graphed on the Clare Chemical Research, Inc., Dark Reader
¢ç
Transilluminator.
°ü·ÃÁ¦Ç°
- No.1 agarose:
SeaKem
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LE Agarose
- »ç¿ëÀÌ °£ÆíÇÑ premade buffer:
Lonza AccuGene
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Buffer ½Ã¸®Áî
- Gradient PCR ±â±â:
TaKaRa PCR Thermal Cycler Touch
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- ³»¿¼º gel maker set¸¦ Æ÷ÇÔÇÑ µðÁöÅÐ Àü±â¿µµ¿ ÀåÄ¡:
Mupid
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-One
Keyword :
¿°»ö½Ã¾à
EtBr º¸´Ù °í°¨µµ·Î ÇÙ»ê °ËÃâ
GelStar¢ç Nucleic Acid Gel Stain
EtBr º¸´Ù °í°¨µµ·Î ÇÙ»ê °ËÃâ
SYBR Green I/II Nucleic Acid Stain
°í°¨µµÀÇ ´Ü¹éÁú ¿°»ö ½Ã¾à
Protein Gel Stains : SYPRO
°í°¨µµÀÇ ´Ü¹éÁú ¿°»ö ½Ã¾à
SYPRO Ruby Protein Stain